Evaluation of methodologies of urine collection to estimate microbial synthesis in bovines diets containing or not containing lipids

Authors

  • Laila Cecília Ramos Bendia Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Carlos Augusto de Alencar Fontes Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Elizabeth Fonsêca Processi Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Clóvis Carlos Silveira Filho Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Cláudio Teixeira Lombardi Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Paulo Roberto Silveira Pimentel Universidade Federal da Bahia
  • Ronaldo Lopes Oliveira Universidade Federal da Bahia
  • Leilson Rocha Bezerra Universidade Federal de Campina Grande
  • Tiago Cunha Rocha Universidade Estadual da Região Tocantina do Maranhão

DOI:

https://doi.org/10.14393/BJ-v36n4a2020-42269

Keywords:

Corn silage, Creatinine excretion, Purine derivatives, Soybeans, Soybean oil.

Abstract

This study investigated the effectiveness of the urine sample collection method in predicting the volume urinary and synthesis of microbial nitrogen. Eight fistulated steers were used with accessible rumens and kept in individual stalls. Their diets consisted of corn silage; corn silage + concentrate; corn silage + concentrate with addition of lipids in the form of soybean oil; and corn silage + concentrate with addition of lipids in the form of soybean grains. Estimates of microbial protein synthesis were obtained based on the urinary excretion of purine derivatives. There was no effect of diets on daily creatinine excretion (P>0.05). There were differences (P<0.05) between the urinary volume and microbial synthesis values determined by the total urine collection and those estimated from the urine spot samples and equations proposed by different authors. The estimation of microbial synthesis based on the urine excretion of purine derivatives should be performed from the total collection of the urine for a period of 24 hours.

 

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Published

2020-05-25

How to Cite

BENDIA, L.C.R.., FONTES, C.A. de A.., PROCESSI, E.F.., SILVEIRA FILHO, C.C.., LOMBARDI, C.T.., PIMENTEL, P.R.S.., OLIVEIRA, R.L.., BEZERRA, L.R.. and ROCHA, T.C., 2020. Evaluation of methodologies of urine collection to estimate microbial synthesis in bovines diets containing or not containing lipids. Bioscience Journal [online], vol. 36, no. 4, pp. 1292–1299. [Accessed10 December 2022]. DOI 10.14393/BJ-v36n4a2020-42269. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/42269.

Issue

Section

Agricultural Sciences