Pollinic preparation for morpho-palynological studies of Passiflora L. Subg. Passiflora L. (Passifloraceae)

Authors

  • Sérgio Alessandro Machado Souza Universidade do Estado de Mato Grosso (UNEMAT)
  • Kellen Coutinho Martins Universidade Estadual do Norte Fluminense Darcy Ribeiro
  • Telma Nair Santana Pereira Universidade Estadual do Norte Fluminense Darcy Ribeiro

DOI:

https://doi.org/10.14393/BJ-v31n4a2015-26240

Keywords:

Pollen morphology, pollen, sodium hydroxide, acetolysis

Abstract

The purpose of this study was to develop a pollen preparation methodology with a high proportion of intact pollen of species for the genus Passiflora subg. Passiflora. Thus, flower buds were collected near anthesis, from the species: P. alata, P. edulis, P. foetida, P. giberti,  and P. setacea, fixed in ethanol-acetic acid mixture (3:1) and treated in 10%-20% aqueous sodium hydroxide, or acetolyzed. The pollen treated with 10% sodium hydroxide resulted in an average of 96% intact pollen grains in all species, however, after acetolysis an average percentage of 98% broken pollen was observed. Data obtained in pollen measurements did not change with the new methodology. It is therefore evident that the proposed methodology for pollen preparation based on sodium hydroxide was effective, as it allowed the visualization of a significant amount of intact pollen, thus promoting morpho-palynological studies of these species.

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Author Biography

Sérgio Alessandro Machado Souza, Universidade do Estado de Mato Grosso (UNEMAT)

Faculdade de Ciências Biológicas e Agrárias, Alta Floresta, MT

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Published

2015-06-30

How to Cite

MACHADO SOUZA, S.A., COUTINHO MARTINS, K. and SANTANA PEREIRA, T.N., 2015. Pollinic preparation for morpho-palynological studies of Passiflora L. Subg. Passiflora L. (Passifloraceae) . Bioscience Journal [online], vol. 31, no. 4, pp. 1200–1204. [Accessed7 December 2022]. DOI 10.14393/BJ-v31n4a2015-26240. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/26240.

Issue

Section

Biological Sciences