First report and molecular characterization of an isolate of papaya ringspot virus (PRSV-W) detected in pumpkin in Cuba

Authors

  • Douglas Rodríguez-Martínez Universidade Federal de Lavras
  • Antonia dos Reis Figueira Univgersidade Federal de Lavras
  • Priscilla de Sousa Geraldino Duarte Universidade Federal de Lavras
  • Suellen Bárbara Ferreira Galvino-Costa Universidade Federal de Lavras
  • Justo González Olmedo Centro de Bioplantas, Universidad de Ciego de Ávila

DOI:

https://doi.org/10.14393/BJ-v31n4a2015-26181

Keywords:

Mosaic, Leaf distortion, Coat protein, HC-Pro, Phylogeny

Abstract

In this work, a virus isolate collected from squash plants (Cucurbita pepo L.), showing severe symptoms of mosaic and leaf deformation, grown in Cuba, was analyzed using indicator plants, electron microscopy, and phylogenetic analysis. Plants of Squash, cv. Caserta, inoculated with this virus isolate showed mosaic, leaf distortion and blistering symptoms, whereas papaya plants were immune and did not show any symptoms. A transmission electron microscopic examination of leaf dip preparations made from infected squash leaves revealed the presence of elongated and flexuous particles, approximately 780-800 x 12 nm in size. Genomic fragments containing the coat protein (CP) and HC-Pro genes, amplified by specific primers for Papaya ringspot virus, W strain (PRSV-W), showed amino acid identities of both genes higher than 94% when compared to other PRSV-W isolates from America. In the phylogenetic tree, this virus isolate has grouped with other virus isolates from America, Australia, and India and was more distant from the Asian isolates. Taken together, the analyses allow the conclusion that this virus isolate is a W strain of PRSV, detected for the first time in Cuba.

Downloads

Download data is not yet available.

Downloads

Published

2015-06-30

How to Cite

RODRÍGUEZ-MARTÍNEZ, D., FIGUEIRA, A. dos R., GERALDINO DUARTE, P. de S., GALVINO-COSTA, S.B.F. and OLMEDO, J.G., 2015. First report and molecular characterization of an isolate of papaya ringspot virus (PRSV-W) detected in pumpkin in Cuba . Bioscience Journal [online], vol. 31, no. 4, pp. 1133–1142. [Accessed26 May 2022]. DOI 10.14393/BJ-v31n4a2015-26181. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/26181.

Issue

Section

Agricultural Sciences