Free activity in the cage associated with body weight gain restores bone structural and mechanical properties in growing rats after hindlimb unloading

Authors

  • Adelton Andrade Barbosa Instituto de Ortopedia e Traumatologia
  • Ricardo Junqueira Del Carlo Universidade Federal de Viçosa
  • Simone Rezende Galvão Universidade Federal de Viçosa
  • Mário Jefferson Quirino Louzada Universidade Estadual Paulista
  • Frederico Souzalima Caldoncelli Franco Instituto Federal de Educação, Ciência e Tecnologia
  • Bárbara Braga Fernandes Universidade Federal de Viçosa
  • Miguel Araújo Carneiro-Junior Universidade Federal do Espírito Santo
  • Regiane Maria Soares Ramos Universidade Federal de Viçosa
  • Lucas Rios Drummond Universidade Federal de Viçosa
  • Antonio Jose Natali Universidade Federal de Viçosa

Abstract

We investigated the importance of daily free activity in the cage and body weight gain during the recovering of bone structural and mechanical properties in growing rats after hindlimb unloading. Eight-week-old male Wistar rats were randomly divided into control (CG, n=24) and suspended (SG, n=24) groups. Animals from SG underwent a four-week hindlimb unloading period by tail-suspension. Animals from CG and those from SG after release were kept in collective cages and sacrificed at the age of 12, 16 and 20 weeks. Both femurs were removed and its area, bone mineral density (BMD), resistance to failure and stiffness were determined. Four-week hindlimb unloading decreased (p<0.05) body weight (CG, 373.00 ± 9.47 vs. SG, 295.86 ± 9.19 g), BMD (CG, 0.19 ± 0.01 vs. SG, 0.15 ± 0.01 g/cm2), bone resistance to failure (CG, 147.75 ± 5.05 vs. SG, 96.40 ± 5.95 N) and stiffness (CG, 0.38 ± 0.01 vs. SG, 0.23 ± 0.02 N/m). Eight weeks of free activity in cage recovered (p>0.05) the body weight (CG, 472.75 ± 14.11 vs. SG, 444.75 ± 18.91 g), BMD (CG, 0.24 ± 0.01 vs. SG, 0.22 ± 0.01 g/cm2), bone resistance to failure (CG, 195.73 ± 10.06 vs. SG, 178.45 ± 8.48 N) and stiffness (CG, 0.56 ± 0.02 vs. SG, 0.47± 0.03 N/m) of SG animals. Body weight correlated strongly with bone structural and mechanical properties (p<0.0001). In conclusion, free activity in the cage associated with body weight gain restored bone structural and mechanical properties in growing rats after hindlimb unloading.

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Published

2012-09-03

How to Cite

BARBOSA, A.A., DEL CARLO, R.J., GALVÃO, S.R., LOUZADA, M.J.Q., FRANCO, F.S.C., FERNANDES, B.B., CARNEIRO-JUNIOR, M.A., RAMOS, R.M.S., DRUMMOND, L.R. and NATALI, A.J., 2012. Free activity in the cage associated with body weight gain restores bone structural and mechanical properties in growing rats after hindlimb unloading . Bioscience Journal [online], vol. 28, no. 4, pp. 660–666. [Accessed4 December 2022]. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/9159.

Issue

Section

Health Sciences