Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook

Authors

  • Maria de Fátima Arrigoni-Blank Universidade Federal de Sergipe http://orcid.org/0000-0002-4907-505X
  • Márcia Raquel Moura Vasconcelos Universidade Federal de Sergipe
  • Rosana Barroso Feitosa-Alcantara Universidade Federal de Sergipe
  • Maria Aparecida Moreira Universidade Federal de Sergipe
  • Evaristo Mauro de Castro Universidade Federal de Lavras
  • Arie Fitzgerald Blank Universidade Federal de Sergipe http://orcid.org/0000-0003-2888-2239

DOI:

https://doi.org/10.14393/BJ-v34n3a2018-39379

Keywords:

Bromeliaceae, Tissue culture, Fertilizer, Leaf anatomy

Abstract

Bromeliads are known worldwide for their ornamental potential. In Brazil, species of the genus Tillandsia occur in the Atlantic rainforest, Amazon rainforest, and rocky fields. This work aimed to evaluate the influence of nitrogen, phosphorus, and potassium fertilization on micropropagated seedlings of Tillandsia bulbosa, at the acclimatization stage, and their leaf anatomy. The experiment was carried out in a completely randomized design, in a 4x4+1 factorial scheme, using coconut coir: earthworm humus: sand mixture as substrate (2:1:1). Urea, single superphosphate, and potassium chloride were used as nitrogen, phosphorus and potassium sources, respectively, at proportions of 50, 100, 200, and 400% of the dose recommended. The doses were distributed in four applications, testing total application at planting (1); ½ application at planting and ½ at 80 DAP (2); ⅓ application at planting, ⅓ at 50 DAP, and ⅓ at 100 DAP (3); and ¼ application at planting, ¼ at 30 DAP, ¼ at 60 DAP, and ¼ at 120 DAP (4); and a control (without fertilization). Leaves anatomy was analyzed at 180 days after planting. Fertilization did not significantly influence the development of seedlings during acclimatization. The doses of 50, 100, and 200% provided thicker parenchyma of chlorophyll and aquifer and leaf blade. Tillandsia bulbosa can be acclimatized without fertilizer application.

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Author Biographies

Maria de Fátima Arrigoni-Blank, Universidade Federal de Sergipe

Departamento de Engenharia Agronômica

Arie Fitzgerald Blank, Universidade Federal de Sergipe

Departamento de Engenharia Agronômica

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Published

2018-05-30

How to Cite

ARRIGONI-BLANK, M. de F., VASCONCELOS, M.R.M., FEITOSA-ALCANTARA, R.B., MOREIRA, M.A., CASTRO, E.M. de and BLANK, A.F., 2018. Influence of N-P-K fertilization at the acclimatization stage on micropropagated seedlings of Tillandsia bulbosa Hook . Bioscience Journal [online], vol. 34, no. 3, pp. 648–656. [Accessed25 May 2022]. DOI 10.14393/BJ-v34n3a2018-39379. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/39379.

Issue

Section

Agricultural Sciences