Diversity of tunisian bread wheat genotypes revealed by morpho-agronomical and microsatellite markers

Authors

  • Elyes Babay Centre de Biotechnologie de Borj-Cédria
  • Ramzi Chaabane Institut National de Recherche Agronomique de Tunisie
  • Rim Mzid-Abdmouleh Centre de Biotechnologie de Borj-Cédria
  • M Ben naceur Institut National de Recherche Agronomique de Tunisie

DOI:

https://doi.org/10.14393/BJ-v31n3a2015-23406

Keywords:

Genetic diversity, Hexaploid Wheat, Morphological Traits, Microsatellites

Abstract

The genetic diversity of a set of 21 hexaploid wheat germplasm from the National Agronomic Institute of Tunisia were investigated by applying 26 agro morphological traits and 10 wheat microsatellites molecular markers (Simple Sequence Repeat). The morphological variability was analyzed using the Principal Component Analysis (PCA) and the cluster analysis based on ward's method and square Euclidean distance. Eighteen microsatellites primer pairs were tested for all genotypes, among them 10 primers generated polymorphic and reproducible profiles. They revealed a total of 414 reducible bands among which 373 were polymorphic. The polymorphic information content (PIC) values per locus varied from 0,33 to 0,94 with an average of 0,72. Genetic similarity values between genotypes, calculated by the molecular derived data, were used to produce a dendrogram. The genotypes were clustered in four clear groups according to their origin, pedigree and in some cases to phenotypic characters similarities.

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Published

2015-05-06

How to Cite

BABAY, E., CHAABANE, R., MZID-ABDMOULEH, R. and BEN NACEUR, M., 2015. Diversity of tunisian bread wheat genotypes revealed by morpho-agronomical and microsatellite markers . Bioscience Journal [online], vol. 31, no. 3, pp. 701–708. [Accessed27 May 2022]. DOI 10.14393/BJ-v31n3a2015-23406. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/23406.

Issue

Section

Agricultural Sciences