Isolation, taxonomic identification and investigation of the biotechnological potential of wild-type Meyerozyma guilliermondii associated with amazonian termites able to ferment D-xylose

Authors

  • Italo Thiago Silveira Rocha Matos Universidade Federal do Amazonas
  • Luciana Araújo Cassa-Barbosa Centro de Biotecnologia da Amazonas
  • Rozana de Souza Medeiros Galvão Universidade Federal do Amazonas
  • Carlos Gustavo Nunes-Silva Universidade Federal do Amazonas
  • Spartaco Astolfi Filho Universidade Federal do Amazonas

Abstract

A wild-type yeast that could ferment D-xylose was isolated from the abdominal content of Nasutitermes sp. collected in the Central Amazon rainforest using sugarcane bagasse hemicellulosic hydrolyzate (SBHH) as selective medium. The yeast was identified as Meyerozyma guilliermondii. Its ability to ferment D-xylose was assessed using liquid medium containing Durham tubes. A fermentometer assay showed a low ethanol yield using D-xylose as the carbon source. Cell viability after heat shock and ethanol shock was 39.8% and 56.0%, respectively. Cultivation in SBHH (pH = 5.0) showed its capability to perform saccharification of this substrate, increasing total reducing sugar concentration to 42.6%. The log phase was observed between 36 and 108 hours of cultivation with a highest specific growth rate (µMAX) of 0.10 h-1. After 120 hours, 79.5% of total reducing sugar was consumed giving a biomass yield of 0.52 g/g. The final pH of SBHH (7.6) showed that M. guilliermondii was able to neutralize the acids of this substrate. These results agree with some predictions in the early eighties, which stated that investigations about microbial content of termite guts would provide new tools for bioconversion of lignocellulosic biomass to fuels and other added-value chemicals. This work is the first report for this species associated with termites in the Amazonian habitat.

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Published

2014-01-17

How to Cite

MATOS, I.T.S.R., CASSA-BARBOSA, L.A., MEDEIROS GALVÃO, R. de S., NUNES-SILVA, C.G. and ASTOLFI FILHO, S., 2014. Isolation, taxonomic identification and investigation of the biotechnological potential of wild-type Meyerozyma guilliermondii associated with amazonian termites able to ferment D-xylose . Bioscience Journal [online], vol. 30, no. 1, pp. 260–266. [Accessed4 December 2022]. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/15024.

Issue

Section

Biological Sciences