Genetic diversity of Piaractus mesopotamicus (Characiformes: Characidae) broodstocks using in the restocking program of Tietê river, Brazil

Authors

  • Maria del Pilar Rodriguez-Rodriguez Universidade Estadual de Maringá
  • Nelson Mauricio Lopera-Barrero Universidade Federal de Mato Grosso
  • Silvio Carlos Alves dos Santos AES Tietê S.A.
  • Lauro Vargas Universidade Estadual de Maringá
  • Danilo Pedro Streit Jr Universidade Federal do Rio Grande do Sul
  • Ricardo Pereira Ribeiro Universidade Estadual de Maringá

Keywords:

Fish, Genetic conservation, Genetic variability, Piaractus mesopotamicus, RAPD, Stocking programs

Abstract

The restocking programs are being used more frequently as methods of fish conservation. However, the reduction in the genetic diversity can affect survival of juveniles used in this programs and cause effects on the wild populations. The aim of this study was assess the genetic diversity in three broodstocks (BA, BB and BC) of pacu Piaractus mesopotamicus of a Hydropower plant in São Paulo - Brazil, using in restocking program of Tietê River. Nine RAPD primers were amplified used extracted DNA from 89 fin-clipping samples. Sixty-nine fragments were polymorphic, 15 had frequencies with significant differences (P<0.05), seven were excluded, and six were fixed fragments. High values for polymorphic fragments (47.83% to 71.01%) and Shannon index (0.270 to 0.424) were observed. Most of the genetic variation was found within the groups through the AMOVA analysis, which was confirmed by the results of the identity and genetic distance. Ancestry levels (FST) among the groups values indicated little and moderate genetic differentiation. The estimate of number of migrants by generation (Nm) indicated levels of gene flow. Moderate genetic divergence between groups (0.214 to 0.259) was observed. The results indicate high (BA and BB) and moderate (BC) variability within broodstocks and genetic differentiation among them. The fish stocks analyzed represent a genetic base that will allow the fish technicians to release juveniles without genetic risks to wild populations present in the river. These genetics procedures can be used as models for other migratory species.

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Author Biographies

Maria del Pilar Rodriguez-Rodriguez, Universidade Estadual de Maringá

Doutoranda do Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá - UEM, Grupo de Pesquisa Peixegen, Centro de Ciências Agrárias - CCA, Av. Colombo, 5790, Bloco 111, Sala 06, CEP 87020-900, Fone/Fax: +55 0xx 44 32614418, Maringá - PR

Nelson Mauricio Lopera-Barrero, Universidade Federal de Mato Grosso

Pesquisador Fapemat/CNPq. Universidade Federal de Mato Grosso - UFMT, Grupo de Pesquisa Peixegen, Instituto de Ciências Agrárias e Tecnológicas, Rod. MT 270, km 06, CEP 78735-901, Fone/Fax: +55 0xx 43 33340813, Rondonópolis - MT

Silvio Carlos Alves dos Santos, AES Tietê S.A.

Gerente de Meio Ambiente. Hidrelétrica AES Tietê - Promissão, Rod. BR 153, km 139 - Brazil, Fone/Fax: +55 0xx 14 35439900 - Promissão, SP

Lauro Vargas, Universidade Estadual de Maringá

Prof. Dr. do Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá - UEM, Grupo de Pesquisa Peixegen, Centro de Ciências Agrárias - CCA, Av. Colombo, 5790, Bloco 111, Sala 06, CEP 87020-900, Fone/Fax: +55 0xx 44 32614418, Maringá - PR

Danilo Pedro Streit Jr, Universidade Federal do Rio Grande do Sul

Prof. Dr. da Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul - UFRGS, Grupo de Pesquisa Peixegen, Av. Bento Gonçalves, 7712, CEP 91540-000, Fone/Fax: +55 0xx 51 33086034, Porto Alegre - RS

Ricardo Pereira Ribeiro, Universidade Estadual de Maringá

Prof. Dr. do Programa de Pós-Graduação em Zootecnia, Universidade Estadual de Maringá - UEM, Grupo de Pesquisa Peixegen, Centro de Ciências Agrárias - CCA, Av. Colombo, 5790, Bloco 111, Sala 06, CEP 87020-900, Fone/Fax: +55 0xx 44 32614418, Maringá - PR

Published

2013-04-02

How to Cite

RODRIGUEZ-RODRIGUEZ, M. del P., LOPERA-BARRERO, N.M., DOS SANTOS, S.C.A., VARGAS, L., STREIT JR, D.P. and RIBEIRO, R.P., 2013. Genetic diversity of Piaractus mesopotamicus (Characiformes: Characidae) broodstocks using in the restocking program of Tietê river, Brazil . Bioscience Journal [online], vol. 29, no. 2. [Accessed26 May 2022]. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/14001.

Issue

Section

Biological Sciences