Effects of Rooibos (Aspalathus linearis) on the Testicular Parenchyma of Adult BALB/C Mice Submitted to a Hypercaloric Diet

Hilara Niemeyer Ruas; João Vitor Lopes-Ferreira; Fernanda Carolina Ribeiro  Dias; Maria Emília Soares Martins dos  Santos; Angelica de Oliveira  Gomes; Mariella Bontempo  Freitas; Marcos de Lucca Moreira Gomes

doi:10.14393/BJ-v39n0a2023-64537

Authors

Hilara Niemeyer Ruas Universidade Federal do Triângulo Mineiro
João Vitor Lopes-Ferreira Universidade Federal do Triângulo Mineiro
Fernanda Carolina Ribeiro Dias Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0002-6530-4684
Maria Emília Soares Martins dos Santos Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0001-8922-0867
Angelica de Oliveira Gomes Universidade Federal do Triângulo Mineiro
Mariella Bontempo Freitas Universidade Federal de Viçosa https://orcid.org/0000-0001-5132-242X
Marcos de Lucca Moreira Gomes Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0003-4397-4609

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-64537

Keywords:

High fat diet, Obesity, Oxidative stress, Reproduction, Testis.

Abstract

Oxidative stress due to obesity plays a detrimental role in the testicular microenvironment and sperm parameters. We explored the impact of a hypercaloric diet in male BALB/c mice as a condition to trigger damage to the spermatogenic process and the antioxidant effect of Aspalathus linearis as well. We used a hypercaloric diet in animals divided into 3 groups: Control, Hypercaloric diet control (HC) and Hypercaloric diet and Rooibos infusion (HCR). Morphometric parameters, enzyme dosages, cell viability, and tubular histopathology were evaluated. Body weight increased in HCR animals at weeks 3, 4, and 8. We found a reduction in seminiferous epithelium height, with an increase in the tubular diameter of the HCR group. Catalase levels were lower in HC and HCR, while carbonyl protein was decreased in HC. We estimate that it induces oxidative stress (OS) capable of affecting the seminiferous epithelium and that the infusion of A. linearis does not demonstrate a potential benefit in cell preservation.

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