Effects of Rooibos (Aspalathus linearis) on the Testicular Parenchyma of Adult BALB/C Mice Submitted to a Hypercaloric Diet

Hilara Niemeyer Ruas; João Vitor Lopes-Ferreira; Fernanda Carolina Ribeiro  Dias; Maria Emília Soares Martins dos Santos; Angelica de Oliveira Gomes; Mariella Bontempo Freitas; Marcos Gomes

doi:10.14393/BJ-v39n0a2023-64537

Autores

Hilara Niemeyer Ruas Universidade Federal do Triângulo Mineiro
João Vitor Lopes-Ferreira Universidade Federal do Triângulo Mineiro
Fernanda Carolina Ribeiro Dias Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0002-6530-4684
Maria Emília Soares Martins dos Santos Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0001-8922-0867
Angelica de Oliveira Gomes Universidade Federal do Triângulo Mineiro
Mariella Bontempo Freitas Universidade Federal de Viçosa https://orcid.org/0000-0001-5132-242X
Marcos Gomes Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0003-4397-4609

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-64537

Palavras-chave:

High fat diet, Obesity, Oxidative stress, Reproduction, Testis.

Resumo

O estresse oxidativo devido à obesidade desempenha um papel prejudicial no microambiente testicular e nos parâmetros espermáticos. Exploramos o impacto de uma dieta hipercalórica em camundongos machos BALB/c como condição para desencadear danos ao processo espermatogênico e testamos o efeito antioxidante de Aspalathus linearis. Utilizou-se dieta hipercalórica em animais divididos em 3 grupos: Controle, HC e HCR. Foram avaliados parâmetros morfométricos, dosagens de enzimas, viabilidade celular e histopatologia tubular. O peso corporal aumentou nos animais HCR nas semanas 3, 4 e 8. Encontramos redução na altura do epitélio seminífero, com aumento do diâmetro tubular do grupo HCR. Os níveis de catalase foram menores em HC e HCR, enquanto a proteína carbonil foi diminuída em HC. Estimamos que induz OS capaz de afetar o epitélio seminífero e que a infusão de A. linearis não demonstra um benefício potencial na preservação celular

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