The role of enzymes in the angiogenic activity of Hancornia speciosa latex

Patricia D'Abadia; Amanda  Fernandes Costa; Myllena Tolentino Firmino Firmino; Samantha Caramori; Ruy de Souza Lino-Junior; Fabiana Fonseca Zanoelo Zanoelo; Pablo José Gonçalves Gonçalves; Luciane Almeida

doi:10.14393/BJ-v38n0a2022-61092

Authors

Patrícia Lima D'Abadia Universidade Estadual de Goiás https://orcid.org/0000-0003-4756-4869
Amanda Fernandes Costa Universidade Estadual de Goiás https://orcid.org/0000-0002-1768-6083
Myllena Tolentino Firmino Universidade Estadual de Goiás https://orcid.org/0000-0002-2807-4760
Samantha Salomão Caramori Universidade Estadual de Goiás https://orcid.org/0000-0003-2676-8280
Ruy de Souza Lino-Junior Universidade Federal de Goiás https://orcid.org/0000-0002-0572-5102
Fabiana Fonseca Zanoelo Universidade Federal do Mato Grosso do Sul https://orcid.org/0000-0002-5778-0322
Pablo José Gonçalves Universidade Federal de Goiás https://orcid.org/0000-0003-0246-1073
Luciane Almeida Universidade Estadual de Goiás https://orcid.org/0000-0003-1764-1480

DOI:

https://doi.org/10.14393/BJ-v38n0a2022-61092

Keywords:

Chick chorioallantoic membrane, Enzymes, Proteinase K

Abstract

The Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response. Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the wound healing process.

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