O papel das enzimas na atividade angiogênica do látex de Hancornia speciosa
DOI:
https://doi.org/10.14393/BJ-v38n0a2022-61092Palavras-chave:
Chick chorioallantoic membrane, Enzymes, Proteinase KResumo
O látex de Hancornia speciosa possui atividade angiogênica, antiinflamatória e antioxidante. Essas propriedades são desejáveis para medicamentos usados para auxiliar na cicatrização de feridas. A angiogênese, foco desta pesquisa, é uma etapa crucial do processo de cicatrização dos tecidos. O objetivo deste trabalho foi compreender o papel das proteínas da fração soro do látex de H. speciosa na angiogênese. Para isso, esse material foi tratado com proteinase K e as proteínas inativadas. Após a inativação, a atividade angiogênica foi avaliada com o uso do ensaio da membrana corioalantóide de embrião de galinha. Como resultado, foi observado que as proteínas presentes na fração soro são responsáveis pela angiogênese. Como as proteínas mostraram importante papel na angiogênese, foi investigado o teor de proteínas totais presentes na fração soro, bem como sua atividade enzimática. O baixo teor de proteína observado no látex da fração sérica de H. speciosa sugere que esse biomaterial possa ser utilizado para o desenvolvimento de novos medicamentos com resposta hipoalergênica. Apesar do baixo teor de proteína, foi identificada atividade enzimática significativa de pelo menos 3 enzimas na fração sérica do látex: β-1,3 glucanase, β-glucosidase e proteases. Em geral, essas enzimas parecem influenciar o processo de cicatrização, auxiliando no desbridamento, ajudando na remodelação da matriz extracelular e deposição de colágeno, diminuindo as chances de contaminação por microrganismos. Em conclusão, o processo de cicatrização de feridas depende não apenas das células e dos fatores de crescimento disponíveis no tecido, mas também de enzimas do microambiente da matriz extracelular.
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Copyright (c) 2022 Patrícia Lima D'Abadia, Amanda Fernandes Costa, Myllena Tolentino Firmino, Samantha Salomão Caramori, Ruy de Souza Lino-Junior, Fabiana Fonseca Zanoelo, Pablo José Gonçalves, Luciane Almeida
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