Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia
DOI:
https://doi.org/10.14393/BJ-v38n0a2022-56064Keywords:
Little Leaf, Periwinkle, Phytoplasma, SEM, Witches’ Broom.Abstract
Cathranthus roseus also known as periwinkle, an ornamental plant contains several medicinal values, was found with the symptoms of little leaf and witches’ broom at Shahjahanpur location with the incidence of up to 8%. The phytoplasma etiology was confirmed through scanning electron microscopy examination in all the four-leaf samples. Molecular analysis through PCR with universal primer pairs P1/P6 followed by nested PCR with R16F2n/R16r2 primers yielded ~1.2kbp amplicons in all the four symptomatic leaf samples. One amplicon was eluted, purified, sequenced, and used in BLASTn searches, which showed maximum identity of periwinkle isolate with several isolates of 16SrIX group of phytoplasma. Further, phylogenic analysis and in silico RFLP confirmed the association of 16SrIX-C subgroup phytoplasma in little leaf and witches broom plants which is the first report from India.
Downloads
References
AHRENS, U., SEEMÜLLER, E. Detection of DNA of plant pathogenic mycoplasma-like organism by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology. 1992, 82, 828-832.
BARBOSA, J.C., et al. Molecular characterization of a phytoplasma of group 16SrIX related to ‘Ca. Phytoplasma phoenicium’ in periwinkle in Brazil. Tropical Plant Pathology. 2021, 37(2), 130-135. https://doi.org/10.1590/S1982-56762012000200005
CHATURVEDI, Y., et al. Association of ‘Candidatus phytoplasma asteris’ with little leaf and phyllody disease of Catharanthus roseus in Eastern Uttar Pradesh, India. Medicinal Plants. 2009, 1(1) 103-108. https://doi.org/10.5958/j.0975-4261.1.2.013
CHEN, W. and LIN, C. Characterization of Catharanthus roseus genes regulated differentially by peanut witches' broom phytoplasma infection. Journal of Phytopathology. 2011, 159, 505-510. https://doi.org/10.1111/j.1439-0434.2011.01796.x
CHO, S.T., CHAN, P.L. and CHIH, H.K. Genomic Characterization of the Periwinkle Leaf Yellowing (PLY) Phytoplasmas in Taiwan. Frontiers in Microbiology. 2019, 10, 2194-2196. https://doi.org/10.3389/fmicb.2019.02194
DENG, S. and HIRUKI, C. Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes. Journal of Microbiological Methods. 1991, 14, 53-61. https://doi.org/10.1016/0167-7012(91)90007-D
GUNDERSEN, D.E. and LEE, I.-M. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea. 1996, 35, 144-151.
LEBSKY, V. and POGHOSYAN, 2014. Scanning electron microscopy detection of phytoplasmas and other phloem limiting pathogens associated with emerging diseases of plants. IN: MÉNDEZ-VILAS, A. (Ed.). Microscopy: advances in scientific research and education. Badajoz, Spain: Formatex Research Center, pp. 78-83.
OMAR, A.F., AMERO, A.E. and JEHAN, M.A. Detection of Phytoplasma Associated with Periwinkle Virescence in Egypt. Plant Pathology Journal. 2008, 7, 92-97.https://doi.org/10.3923/ppj.2008.92.97
RAO, G.P., et al. Century progress of research on phytoplasma diseases in India. Phytopathogenic Mollicutes. 2017, 7(1), 1-38. https://doi.org/10.5958/2249-4677.2017.00001.9
PRIYA, M., TIWARI, A.K. and RAO, G.P. First molecular identification of ‘Candidatus Phytoplasma trifolii’ (16SrVI-D) in Croton bonplandianum baill from India. Journal of Plant Pathology. 2016, 98(1), 171-185. http://dx.doi.org/10.4454/JPP.V98I1.060
SHUKLA, K., et al. Association of pigeon pea witches’ broom phytoplasma infecting Carpobrotus edulis (L.) N.E. Br in India. Phytopathogenic Mollicutes. 2014, 4, 27-32. https://doi.org/10.5958/2249-4677.2014.00579.9
TAMURA, K., et al. MEGA 6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution. 2013, 30(12), 2725-2729. https://doi.org/10.1093/molbev/mst197
TIWARI, N.N., JAIN, R.K. and TIWARI, A.K. Hishimonusphycitis: possible vector of 16SrI-B subgroup phytoplasma. Agrica. 2021, 10(1), 72-75. https://doi.org/10.5958/2394-448X.2021.00005.5
ZHAO, Y., et al. Construction of an interactive online phytoplasama classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology. 2009, 59, 2582-2593. https://doi.org/10.1099/ijs.0.010249-0
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Niraj Nath Tiwari, Ravindra Kumar Jain, Ajay Kumar Tiwari
This work is licensed under a Creative Commons Attribution 4.0 International License.
