Detecção de 'CA. Phytoplasma phoenicium' em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia

Autores

  • Niraj Nath Anand Engineering College
  • Ravindra Kumar Jain Anand Engineering College
  • Ajay Kumar Tiwari Sugarcane Research Institute

DOI:

https://doi.org/10.14393/BJ-v38n0a2022-56064

Palavras-chave:

Little Leaf, Periwinkle, Phytoplasma, SEM, Witches’ Broom.

Resumo

Cathranthus roseus também conhecida como pervinca, planta ornamental contém vários valores medicinais, foi encontrada com poucos sintomas de folhas e vassouras de bruxa no local de Shahjahanpur, com incidência de até 8%. A etiologia do fitoplasma foi confirmada através do exame de microscopia eletrônica de varredura. Análises moleculares adicionais por meio de PCR aninhado, análise filogênica e silco RFLP confirmaram a associação do fitoplasma do subgrupo 16SrIX-C em pequenas plantas de folhas e vassouras de bruxa, que é o primeiro relatório da Índia.

Downloads

Não há dados estatísticos.

Referências

AHRENS, U., SEEMÜLLER, E. Detection of DNA of plant pathogenic mycoplasma-like organism by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology. 1992, 82, 828-832.

BARBOSA, J.C., et al. Molecular characterization of a phytoplasma of group 16SrIX related to ‘Ca. Phytoplasma phoenicium’ in periwinkle in Brazil. Tropical Plant Pathology. 2021, 37(2), 130-135. https://doi.org/10.1590/S1982-56762012000200005

CHATURVEDI, Y., et al. Association of ‘Candidatus phytoplasma asteris’ with little leaf and phyllody disease of Catharanthus roseus in Eastern Uttar Pradesh, India. Medicinal Plants. 2009, 1(1) 103-108. https://doi.org/10.5958/j.0975-4261.1.2.013

CHEN, W. and LIN, C. Characterization of Catharanthus roseus genes regulated differentially by peanut witches' broom phytoplasma infection. Journal of Phytopathology. 2011, 159, 505-510. https://doi.org/10.1111/j.1439-0434.2011.01796.x

CHO, S.T., CHAN, P.L. and CHIH, H.K. Genomic Characterization of the Periwinkle Leaf Yellowing (PLY) Phytoplasmas in Taiwan. Frontiers in Microbiology. 2019, 10, 2194-2196. https://doi.org/10.3389/fmicb.2019.02194

DENG, S. and HIRUKI, C. Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes. Journal of Microbiological Methods. 1991, 14, 53-61. https://doi.org/10.1016/0167-7012(91)90007-D

GUNDERSEN, D.E. and LEE, I.-M. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopathologia Mediterranea. 1996, 35, 144-151.

LEBSKY, V. and POGHOSYAN, 2014. Scanning electron microscopy detection of phytoplasmas and other phloem limiting pathogens associated with emerging diseases of plants. IN: MÉNDEZ-VILAS, A. (Ed.). Microscopy: advances in scientific research and education. Badajoz, Spain: Formatex Research Center, pp. 78-83.

OMAR, A.F., AMERO, A.E. and JEHAN, M.A. Detection of Phytoplasma Associated with Periwinkle Virescence in Egypt. Plant Pathology Journal. 2008, 7, 92-97.https://doi.org/10.3923/ppj.2008.92.97

RAO, G.P., et al. Century progress of research on phytoplasma diseases in India. Phytopathogenic Mollicutes. 2017, 7(1), 1-38. https://doi.org/10.5958/2249-4677.2017.00001.9

PRIYA, M., TIWARI, A.K. and RAO, G.P. First molecular identification of ‘Candidatus Phytoplasma trifolii’ (16SrVI-D) in Croton bonplandianum baill from India. Journal of Plant Pathology. 2016, 98(1), 171-185. http://dx.doi.org/10.4454/JPP.V98I1.060

SHUKLA, K., et al. Association of pigeon pea witches’ broom phytoplasma infecting Carpobrotus edulis (L.) N.E. Br in India. Phytopathogenic Mollicutes. 2014, 4, 27-32. https://doi.org/10.5958/2249-4677.2014.00579.9

TAMURA, K., et al. MEGA 6: molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution. 2013, 30(12), 2725-2729. https://doi.org/10.1093/molbev/mst197

TIWARI, N.N., JAIN, R.K. and TIWARI, A.K. Hishimonusphycitis: possible vector of 16SrI-B subgroup phytoplasma. Agrica. 2021, 10(1), 72-75. https://doi.org/10.5958/2394-448X.2021.00005.5

ZHAO, Y., et al. Construction of an interactive online phytoplasama classification tool, iPhyClassifier, and its application in analysis of the peach X-disease phytoplasma group (16SrIII). International Journal of Systematic and Evolutionary Microbiology. 2009, 59, 2582-2593. https://doi.org/10.1099/ijs.0.010249-0

Downloads

Publicado

2022-03-31

Como Citar

NIRAJ NATH, JAIN, R.K. e AJAY KUMAR TIWARI, 2022. Detecção de ’CA. Phytoplasma phoenicium’ em plantas de pervinca (Cathranthus roseus) em Uttar Pradesh, Índia. Bioscience Journal [online], vol. 38, pp. e38026. [Accessed22 novembro 2024]. DOI 10.14393/BJ-v38n0a2022-56064. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/56064.

Edição

Seção

Ciências Agrárias