Study on extraction conditions and characteristics of protein from burdock roots

XiuMei Gong; Fei Chang; XiMin Yao; Peng Leng; YuQing Miao; GuiZhi Zhang

doi:10.14393/BJ-v40n0a2024-70783

Authors

XiuMei Gong Linyi University
Fei Chang Linyi University https://orcid.org/0009-0004-0484-3619
XiMin Yao Linyi Academy of Agricultural Sciences
Peng Leng Linyi Academy of Agricultural Sciences
YuQing Miao Linyi University
GuiZhi Zhang Linyi University https://orcid.org/0009-0004-6700-8514

DOI:

https://doi.org/10.14393/BJ-v40n0a2024-70783

Keywords:

Acid precipitation, Alkali extraction, Amino acid content, Burdock, Protein characteristics, Protein extraction.

Abstract

Burdock (Arctium lappa L.), which belongs to the Compositae family, possesses significant medicinal and nutritional value. Burdock root is abundant in proteins and essential amino acids. In this study, proteins from burdock roots were extracted by alkali extraction and acid sedimentation, and the optimal conditions for protein extraction from burdock roots and protein characteristics were explored. The optimal conditions for protein extraction with a yield rate of 9.03% were 40°C extraction temperature, pH 8.0, a solid–liquid ratio of 1:25 (g/mL), and extraction time of 65 minutes. Single-factor and orthogonal tests on the extraction rate of burdock proteins under different conditions found that the extraction rate of protein from burdock roots were influenced by pH, extraction temperature, solid–liquid ratio, and extraction time, in this order. The solubility and gelation properties of burdock protein were similar to those of soybean protein; however, burdock protein demonstrated 1.2 times the oil absorption capacity and half the water retention capacity of soybean protein. Analysis of the amino acid contents of burdock protein revealed that burdock root contains significant essential and non-essential amino acids, with valine content being the highest.

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References

BARUA, S., KIM, H., HONG, S. C. et al. Moisturizing effect of serine-loaded solid lipid nanoparticles and polysaccharide-rich extract of root Phragmites communis incorporated in hydrogel bases. Archives of pharmacal research, 2017, 40(2), 250–257. https://doi.org/10.1007/s12272-016-0846-1

BOUKID, F., PASQUALONE, A. Lupine (Lupinus spp.) proteins: characteristics, safety and food applications. European Food Research and Technology. 2021, 248, 345-356. http://hdl.handle.net/20.500.12327/1530

CHAN, Y. S., CHENG, L. N., WU, J. H., et al. A review of the pharmacological effects of Arctium lappa (burdock). Inflammopharmacology. 2011, 19(5), 245-54. https://doi.org/10.1007/s10787-010-0062-4

DONG, J., ZHAO, J., LIN, Y., et al. Exercise improves recognition memory and synaptic plasticity in the prefrontal cortex for rats modelling vascular dementia. Neurol Res. 2018, 40(1), 68-77. https://doi.org/10.1080/01616412.2017.1398389

EICK, S., and LUSSI, A. Arginine: A Weapon against Cariogenic Biofilm?. Monographs in oral science, 2021, 29, 80–90. https://doi.org/10.1159/000510203

HA, M. S., YOOK, J. S., LEE, M., et al. Exercise training and burdock root (Arctium lappa L.) extract independently improve abdominal obesity and sex hormones in elderly women with metabolic syndrome. Scientific Reports. 2021, 11(1), 5175. https://doi.org/10.1038/s41598-021-84301-x

HEINZ, A. Elastic fibers during aging and disease. Ageing research reviews, 2021, 66, 101255. https://doi.org/10.1016/j.arr.2021.101255

Herrera-Balandrano, D. D., Beta, T., Chai, Z., et al. Effect of in vitro gastro-intestinal digestion on the phenolic composition and antioxidant capacity of Burdock roots at different harvest time. Food Chemistry. 2021, 358, 129897. https://doi.org/10.1016/j.foodchem.2021.129897

JACOBI, S. K., and ODLE, J. Nutritional factors influencing intestinal health of the neonate. Adv Nutr. 2012, 3(5), 687-96. https://doi.org/10.3945/an.112.002683

JAEGER, A., ZANNINI, E., SAHIN, A. W., et al. Barley Protein Properties, Extraction and Applications, with a Focus on Brewers’ Spent Grain Protein. Foods. 2021, 10(6), 1389-1389. https://doi.org/10.3390/foods10061389

JIANFENG, C., PENGYING, Z., CHENGWEI, X., et al. Effect of aqueous extract of Arctium lappa L. (burdock) roots on the sexual behavior of male rats. BMC Complement Altern Med. 2012, 12, 8. https://doi.org/10.1186/1472-6882-12-8

KUMAR, M., PRAKASH, S., RADHA, K., et al. Beneficial Role of Antioxidant Secondary Metabolites from Medicinal Plants in Maintaining Oral Health. Antioxidants (Basel, Switzerland). 2021, 10(7), 1061. https://doi.org/10.3390/antiox10071061

KUMAR, M., TOMAR, M., PUNIA, S., et al. Plant-based proteins and their multifaceted industrial applications. Lwt-Food Science And Technology. 2022, 154, 112620. https://doi.org/10.1016/j.lwt.2021.112620

LANGYAN, S., YADAVA, P., KHAN, F. N., et al. Sustaining Protein Nutrition Through Plant-Based Foods. Frontiers in nutrition. 2022, 8, 772573. https://doi.org/10.3389/fnut.2021.772573

LEE, J., HA, S. J., PARK, J., et al. Arctium lappa root extract containing L-arginine prevents TNF-α-induced early atherosclerosis in vitro and in vivo. Nutr Res. 2020, 77, 85-96. https://doi.org/10.1016/j.nutres.2020.03.003

LEWIS, J. D., DE JONG, M. E., BAGHA, S. M., et al. All roads lead to arginine: the squid protamine gene. J Mol Evol. 2004, 58(6), 673-80. https://doi.org/10.1007/s00239-004-2589-8

LI, Z., XU, C., YU, H., et al. Effects of Dietary Cystine and Tyrosine Supplementation on Melanin Synthesis in the Pacific Oyster (Crassostrea gigas). Marine biotechnology (New York, N.Y.), 2023, 25(4), 537–547. https://doi.org/10.1007/s10126-023-10223-6

MNAB C, FS B, RAC D et al. Effect of Arctium lappa linne (Burdock) root tea consumption on lipid profile and blood pressure in patients with knee osteoarthritis - ScienceDirect. Journal of Herbal Medicine. 2019, 17–18(2), 100266. https://doi.org/10.1016/j.hermed.2019.100266

MONDAL, S. C., and EUN, J. B. Mechanistic insights on burdock (Arctium lappa L.) extract effects on diabetes mellitus. Food science and biotechnology. 2022, 31(8), 999–1008. https://doi.org/10.1007/s10068-022-01091-2

MORO, T. M. A., and T P S CLERICI, M. Burdock (Arctium lappa L) roots as a source of inulin-type fructans and other bioactive compounds: Current knowledge and future perspectives for food and non-food applications. Food Research International. 2020, 121, 109889. https://doi.org/10.1016/j.foodres.2020.109889

OBOH G. Phytomedicine and functional foods: Keys to sustainable healthcare delivery. Journal of Food Biochemistry. 2021, 45(3), 13634. https://doi.org/10.1111/jfbc.13634

PREDES, F. S., RUIZ, A. L., CARVALHO, J. E., et al. Antioxidative and in vitro antiproliferative activity of Arctium lappa root extracts. BMC Complement Altern Med. 2011, 11, 25. https://doi.org/10.1186/1472-6882-11-25

PRINSLOO, G., MAROKANE, C. K., & STREET, R. A. Anti-HIV activity of southern African plants: Current developments, phytochemistry and future research. J Ethnopharmacol. 2018, 210, 133-155. https://doi.org/10.1016/j.jep.2017.08.005

REN, X., ZOU, Q., YUAN, C., et al. The Dominant Role of Oxygen in Modulating the Chemical Evolution Pathways of Tyrosine in Peptides: Dityrosine or Melanin. Angewandte Chemie (International ed. in English), 2019, 58(18), 5872–5876. https://doi.org/10.1002/anie.201814575

RITTERHOFF, J., YOUNG, S., VILLET, O., et al. Metabolic Remodeling Promotes Cardiac Hypertrophy by Directing Glucose to Aspartate Biosynthesis. Circulation research. 2020, 126(2), 182–196. https://doi.org/10.1161/CIRCRESAHA.119.315483

ROMERO-GÓMEZ, M. Role of phosphate-activated glutaminase in the pathogenesis of hepatic encephalopathy. Metabolic brain disease, 2005, 20(4), 319–325. https://doi.org/10.1007/s11011-005-7913-5

ROMUALDO, G. R., SILVA, E. D. A., DA SILVA, T. C., et al. Burdock (Arctium lappa L.) root attenuates preneoplastic lesion development in a diet and thioacetamide-induced model of steatohepatitis-associated hepatocarcinogenesis. Environ Toxicol. 2020, 35(4), 518-527. https://doi.org/10.1002/tox.22887

SKOWROŃSKA, W., GRANICA, S., DZIEDZIC, M., et al. Arctium lappa and Arctium tomentosum, Sources of Arctii radix: Comparison of Anti-Lipoxygenase and Antioxidant Activity as well as the Chemical Composition of Extracts from Aerial Parts and from Roots. Plants (Basel). 2021, 10(1), 78. https://doi.org/10.3390/plants10010078

SZPARAGA, A., KOCIRA, S., & KAPUSTA, I. Identification of a Biostimulating Potential of an Organic Biomaterial Based on the Botanical Extract from Arctium lappa L. Roots. Materials (Basel). 2021, 14(17), 4920. https://doi.org/10.3390/ma14174920

URAZOVA, L. N., KUZNETSOVA, T. I., BOEV, R. S., et al. Efficacy of natural L-asparagine in the complex therapy for malignant tumors in researchal studies. Exp Oncol. 2011, 33(2), 90-3. http://dspace.nbuv.gov.ua/handle/123456789/138631

WU, K. C., WENG, H. K., HSU, Y. S., et al. Aqueous extract of Arctium lappa L. root (burdock) enhances chondrogenesis in human bone marrow-derived mesenchymal stem cells. BMC Complement Med Ther. 2020, 20(1), 364. https://doi.org/10.1186/s12906-020-03158-1

YOSRI, N., ALSHARIF, S. M., XIAO, J., et al. Arctium lappa (Burdock): Insights from ethnopharmacology potential, chemical constituents, clinical studies, pharmacological utility and nanomedicine. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2023, 158, 114104. https://doi.org/10.1016/j.biopha.2022.114104

ZHANG, J., KANG, D., ZHANG, W., et al. Recent advantage of interactions of protein-flavor in foods: Perspective of theoretical models, protein properties and extrinsic factors. Trends in Food Science & Technology. 2021, 111, 405-425. https://doi.org/10.1016/j.tifs.2021.02.060

ZHANG, M., WANG, Y. W., ZHU, Y. Z., et al. Discovery of quality control ingredients in burdock root by combining anti-tumor effects and UHPLC-QqQ-MS/MS. Biomed Chromatogr. 2021, 35(11), e5187. https://doi.org/10.1002/bmc.5187]

ZHANG, X., HERRERA-BALANDRANO, D. D., HUANG, W., et al. Comparison of Nutritional and Nutraceutical Properties of Burdock Roots Cultivated in Fengxian and Peixian of China. Foods (Basel, Switzerland). 2021, 10(9), 2095. https://doi.org/10.3390/foods10092095

ZHENG, Z., XUE, Y., JIA, J., et al. Tiopronin-induced membranous nephropathy: a case report. Renal failure. 2014, 36(9), 1455–1460. https://doi.org/10.3109/0886022X.2014.926754