Improving accelerated aging and electrical conductivity tests to assess popcorn seed vigor

Authors

  • Brenda Santos Pontes Universidade Federal de Uberlândia
  • Hugo César Rodrigues Moreira Catão Universidade Federal de Uberlândia https://orcid.org/0000-0002-6232-6351
  • Maurício Alves de Oliveira Filho Universidade Federal de Uberlândia https://orcid.org/0000-0002-9268-1637
  • Ana Laura Costa Santos Universidade Federal de Uberlândia
  • Rossana Bertaglia Zanetti Universidade Federal de Uberlândia

DOI:

https://doi.org/10.14393/BJ-v41n0a2025-69965

Keywords:

Salt solution, Soaking period, Vigor, Zea mays L. var. everta.

Abstract

Laboratories constantly evaluate seed lot quality under controlled conditions; however, these tests may overestimate actual field values, making vigor tests indispensable. This study fine-tuned the methods for testing electrical conductivity and accelerated aging to determine popcorn seed vigor. Four lots of hybrid AP8203 popcorn seeds underwent testing for the parameters of germination, first germination count, seedling length, emergence, emergence speed index, water content, electrical conductivity (immersion of 50 or 100 seeds in 50, 75, and 100 ml of distilled water for two, four, five, eight, and 24 hours), and accelerated aging (conventional with water, unsaturated NaCl solution, and saturated NaCl solution). The study had a completely randomized design. The accelerated aging test efficiently aged popcorn seeds using a conventional solution for 48 hours and a saturated saline solution for 72 hours. The electrical conductivity test effectively discriminated seed lots into vigor classes when using 50 popcorn seeds in 50 ml of water at 25°C after six hours of imbibition.

References

ALVARENGA, E.M., et al. Teste de condutividade elétrica para avaliar o vigor de sementes de milho-pipoca. Informativo Abrates. 2003, 13(3), 275.

ARAÚJO, J. O., et al. Adjustment of the electrical conductivity test to evaluate the seed vigor of chickpea (Cicer arietinum L.). Journal of Seed Science. 2022, 44, 1-11. http://dx.doi.org/10.1590/2317-1545v44258666

BEWLEY, J.D., BLACK, M. Seeds: physiology of development, germination and dormancy. 3ª ed. New York: Springer, 2014.

BRASIL. Instrução Normativa Nº 45, de 17 de Setembro de 2013. Brasília: Diário Oficial [da] República Federativa do Brasil, 2013.

CATÃO, H.C.R.M., CAIXETA, F. Physiological, isozyme changes and image analysis of popcorn seeds submitted to low temperatures. Journal of Seed Science. 2017, 39(3), 234-243. https://doi.org/10.1590/2317-1545v39n3169853

CATÃO, H.C.R.M., et al. Antioxidant activity and physiological performance of popcorn seed after saline stress and analysis of seedling images. Ciência e Agrotecnologia. 2020, 44, 2. http://dx.doi.org/10.1590/1413-7054202044005020

CATÃO, H.C.R.M., et al. Improving the method for determining the physiological and sanitary potential of gherkin seeds. Bioscience Journal. 2021, 37, 1-12. https://doi.org/10.14393/BJ-v37n0a2021-54193

FERREIRA, D.F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia. 2011, 35(6), 1039-1042. https://doi.org/10.1590/S1413-70542011000600001

FREITAS, R.M.O., et al. Accelerated aging of arugula seeds. Revista Brasileira de Ciências Agrárias. 2018, 13(4), 1-6. https://doi.org/10.5039/agraria.v13i4a5585

GRZYBOWSKI, C. R. S., VIEIRA, R. D., PANOBIANCO M. Testes de estresse na avaliação do vigor de sementes de milho. Revista Ciência Agronômica [Internet]. 2015, 46(3), 590–6. https://doi.org/10.5935/1806-6690.20150042

GUEDES, R.S., et al. Envelhecimento acelerado na avaliação da qualidade fisiológica de sementes de Dalbergia nigra (Vell.) Fr. All. Semina: Ciências Agrárias. 2011, 32(2), 443-450.

HAMPTON, J.G., TEKRONY, D.M. Handbook of vigour test methods. In: Seed vigour testing. In: International Rules for Seed Testing. Zurich: ISTA, 1995.

KHAN, J., et al. (2020) Amelioration of ageing associated alterations and oxidative inequity in seeds of Cicer arietinum by silver nanoparticles. Journal Of Plant Growth Regulation. 2020, 40(3), 1341-1351. https://doi.org/10.1007/s00344-020-10193-2

KRZYZANOWSKI, F. C., et al. Teste de condutividade elétrica para avaliar o vigor da semente de soja. Circular Técnica 199. Londrina: Embrapa Soja. 2023, 20. Disponível em: https://www.infoteca.cnptia.embrapa.br/infoteca/bitstream/doc/1158188/1/Circ-Tec-199.pdf

KRZYZANOWSKI, F.C., et al. Vigor de Sementes: Conceitos e Testes. 2ª ed. Londrina: ABRATES, 2020.

LEITE, M.S., et al. Classification of West Indian gherkin seeds vigor by respiratory activity. Revista Ciência Agronômica. 2019, 50(2), 307-311. https://doi.org/10.5935/1806-6690.20190036

LIMA, J.J.P., et al. Accelerated aging and electrical conductivity tests in crambe seeds. Ciência Agrotecnologia. 2015, 39(1), 7-14. https://doi.org/10.1590/S1413-70542015000100001

MAGUIRE, J.D. Speeds of germination-aid selection and evaluation for seedling emergence and vigor. Crop Science. 1962, 2(1), 176-177. https://doi.org/10.2135/cropsci1962.0011183X000200020033x

MARCOS-FILHO, J. Fisiologia de sementes de plantas cultivadas. Piracicaba: FEALQ, 2015.

MARCOS-FILHO, J. Seed vigor testing: an overview of the past, present and future perspective. Scientia Agricola. 2015a, 72(4), 363-374. https://doi.org/10.1590/0103-9016-2015-0007

MARQUES RODRIGUES C., et al. Atividade respiratória como teste de vigor em sementes de feijão (Phaseolus vulgaris L.). Div Journ [Internet]. 2019, 4(3), 1070-81. https://doi.org/10.17648/diversitas-journal-v4i3.853

MARQUES, A. R., DUTRA, A. S. Metodologia do teste de condutividade elétrica em sementes de sorgo granífero. Revista Brasileira de Milho e Sorgo. 2018, 17(1), 147-156. https://doi.org/10.18512/1980-6477/rbms.v17n1p147-156

MORAES, C.E., et al. Qualidade fisiológica de sementes de Tabernaemontana fuchsiaefolia A. DC em função do teste de envelhecimento acelerado. Ciência Florestal. 2016, 26(1), 213 – 223. https://doi.org/10.5902/1980509821114

MOURA, M.C.F., et al. Teste da condutividade elétrica na avaliação fisiológica em sementes de Vigna unguiculata. Revista de Ciências Agrárias. 2017, 40(2), 714-721. https://doi.org/10.19084/RCA17034

MUNARETO, J. D., et al. Adaptação do Teste de Envelhecimento Acelerado em Sementes de Quinoa. Ensaios e Ciência C Biológicas Agrárias e Da Saúde. 2021, 25(5), 564–569. https://doi.org/10.17921/1415-6938.2021v25n5-esp.p564-569

OLIVEIRA, A. M. S., et al. Accelerated aging for evaluation of vigor in Brachiaria brizantha ‘Xaraés’ seeds. Journal of Seed Science. 2020, 42, e202042006. https://doi.org/10.1590/2317-1545v42216691

PRADO, J. P., et al. Physiological potential of soybean seeds and its relationshipto electrical conductivity. J Seed Sci [Internet]. 2019, 41(4), 407–15. https://doi.org/10.1590/2317-1545v41n4214988

RADKE, A.K., et al. Alternativas metodológicas do teste de envelhecimento acelerado em sementes de coentro. Ciência Rural. 2016, 46(1), 95-99. https://doi.org/10.1590/0103-8478cr20140188

RECH CASSOL, F.D., et al. Biochemical behavior of bean seeds and grains during storage. Bioscience Journal [online]. 2016, 32(6), 1545–1551. https://doi.org/10.14393/BJ-v32n6a2016-33077

ROCHA, C.S., et al. Physiological quality of popcorn seeds assessed by the accelerated aging test. Journal of Seed Science. 2018, 40(4), 428-434. http://dx.doi.org/10.1590/2317-1545v40n4191101

RODRIGUES, M. et al. Physiological performance of soybean seeds in the accelerated aging test and their germination after several waiting periods. Journal Of Seed Science. 2022, 44, 1-8. http://dx.doi.org/10.1590/2317-1545v44257868.

SANTOS, R. F. et al. Accelerated aging methodologies for evaluating physiological potential of treated soybean seeds. Journal of Seed Science. 2021, 43, e202143028. https://doi.org/10.1590/2317-1545v43250605

SILVA, S.S., et al. Electrical conductivity of different common bean seeds genotypes. Journal of Seed Science. 2013, 35(2), 216-224. https://www.scielo.br/j/jss/a/wdwVhGGQQP45gjVSdVFGPJP/

SOUZA, G.E., et al. Comparação entre métodos para a avaliação do vigor de sementes de algodão. Revista de Agricultura Neotropical. 2014, 1(2), 35-41. https://doi.org/10.32404/rean.v1i2.237

SUZANA, C. S., et al. Qualidade fisiológica e sanitária de sementes salvas de feijão produzidas no médio alto Uruguai, RS, Brasil. Agrotrópica (Itabuna). 2017, 29(2), 157-166. http://dx.doi.org/10.21757/0103-3816.2017v29n2p157-166

TORRES, S.B., et al. Teste de condutividade elétrica na avaliação da qualidade fisiológica de sementes de coentro. Revista Ciência Agronômica. 2015, 46(3), 622-629. https://doi.org/10.5935/1806-6690.20150046

VIEIRA, R.D.; MARCOS-FILHO, J. Teste de condutividade elétrica. In: KRZYZANOWSKI, F.C.; VIEIRA, R.D.; FRANÇA-NETO, J.B.; MARCOSFILHO, J. (Eds.). Vigor de sementes: conceitos e testes. Londrina: ABRATES, 2020.

WENDT, L., et al. Relação entre testes de vigor com a emergência a campo em sementes de soja. Revista Brasileira de Ciências Agrárias. 2017, 12(2), 166-171. https://doi.org/10.5039/agraria.v12i2a5435

Downloads

Published

2025-04-02

Issue

Section

Agricultural Sciences

How to Cite

Improving accelerated aging and electrical conductivity tests to assess popcorn seed vigor. Bioscience Journal [online], 2025. [online], vol. 41, pp. e41009. [Accessed5 June 2025]. DOI 10.14393/BJ-v41n0a2025-69965. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/69965.