An efficient protocol for in vitro regeneration of peanut (Arachis hypogaea L.) cultivar L14

Phung Thi Bich  Hoa; Nguyen Hoang  Tue; Phan Thi Quyen  Trang; Le Thi  Hang; Nguyen Quang Duc  Tien; Nguyen Hoang  Loc

doi:10.14393/BJ-v37n0a2021-56949

Authors

Phung Thi Bich Hoa Hue University https://orcid.org/0000-0002-9989-7713
Nguyen Hoang Tue Hue University https://orcid.org/0000-0003-4387-0201
Phan Thi Quyen Trang Hue University https://orcid.org/0000-0001-5699-3169
Le Thi Hang Hue University https://orcid.org/0000-0002-9570-3547
Nguyen Quang Duc Tien Hue University https://orcid.org/0000-0002-7330-3139
Nguyen Hoang Loc Hue University https://orcid.org/0000-0002-6387-0359

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-56949

Keywords:

Arachis hypogaea, In Vitro Propagation, Rooting, Shoot Multiplication, Shoot Regeneration.

Abstract

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.

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