Survival capacity of Arcobacter butzleri at different incubation temperatures in drinking water

Ana Laura Rodríguez Hidalgo; María Laura Arias Echandi; Eduardo Castro Arias; Heriberto  Fernandez Jaramillo

doi:10.14393/BJ-v38n0a2022-50398

Authors

Ana Laura Rodríguez Hidalgo University of Costa Rica https://orcid.org/0000-0001-9583-3635
María Laura Arias Echandi University of Costa Rica https://orcid.org/0000-0002-8611-4433
Eduardo Castro Arias Medical Sciences University UCIMED https://orcid.org/0000-0002-0929-0177
Heriberto Fernandez Jaramillo Universidad Austral de Chile https://orcid.org/0000-0002-0009-7258

DOI:

https://doi.org/10.14393/BJ-v38n0a2022-50398

Keywords:

Arcobacter butzleri, Drinking water, Survival.

Abstract

Arcobacter represents a zoonotic emerging pathogen with increasing importance for public health and drinking water has been cited as a major risk factor for its dissemination. The aim of this work was to evaluate the survival capacity of Arcobacter in different water matrixes stored at different temperatures. Three different water matrixes were used, including potable water with a chlorine concentration of 0,5 mg/mL, non-chlorinated water and non-chlorinated water added with an 11% of organic matter. Each matrix was inoculated in a 1/10 proportion with 103 and 105 Arcobacter pools, divided into 4 different subsamples, in order to be incubated at 0°C, 5°C, 12 °C and 25°C by up to 15 days. The presence of Arcobacter in each matrix was determined on days 1, 3, 5, 7, 9, 11, 13 and 15. Results obtained show that this bacterium can survive in all the water matrixes evaluated, regardless of the presence or not of residual disinfecting agent. Also, the amount of CFU/mL inoculated in water correlates with the number of bacteria that can survive on it, and that incubation temperature has a significant effect over the bacterial survival.

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