Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization

Authors

  • Adelaide Siqueira Silva Universidade Federal de Uberlândia
  • José Magno Queiroz Luz Universidade Federal de Uberlândia
  • Nilvanira Donizete Tebaldi Universidade Federal de Uberlândia
  • Tâmara Prado de Morais Universidade Federal de Uberlândia

DOI:

https://doi.org/10.14393/BJ-v36n2a2020-46256

Keywords:

Phytobacteria, Potato blackleg, Polymerase chain reaction, Solanum tuberosum

Abstract

Pectobacterium is a complex taxon of strains with diverse characteristics. It comprises several genera, including Erwinia, Brenneria, Pectobacterium, Dickeya, and Pantoea. Pectobacterium and Dickeya cause diseases in a wide range of plants, including potatoes, where they are causative agents of soft rot in tubers and blackleg in field-grown plants. Characterizing Pectobacterium species allows for the analysis of the diversity of pectinolytic bacteria, which may support control strategies for plant bacterial diseases. The aim of this study was to perform biochemical, physiological, and molecular characterizations of Pectobacterium spp. from different sites and host plants. The isolated strains were characterized by the glucose fermentation test, Gram staining, catalase activity, oxidase activity, growth at 37 ºC, reducing substances from sucrose, phosphatase activity, indole production, acid production from different sources (sorbitol, melibiose, citrate, and lactose), pathogenicity in potato, and hypersensitivity reactions. Molecular characterization was performed with species-specific primers ECA1f/ECA2r and EXPCCF/EXPCCR, which identify P. atrosepticum and P. carotovorum subsp. carotovorum (Pcc), respectively, and with primers 1491f/L1RA/L1RG and Br1f/L1RA/L1RG that differentiate Pcc from Dickeya chrysanthemi and from P. carotovorum subsp. brasiliensis. The strains were identified as belonging to the genus Pectobacterium, though they did not fit the biochemical nor the molecular classification standards for subspecies differentiation, indicating significant diversity among the strains.

Downloads

Download data is not yet available.

Downloads

Published

2020-02-11

How to Cite

SILVA, A.S., LUZ, J.M.Q., TEBALDI, N.D. and MORAIS, T.P. de, 2020. Diversity of Pectobacterium strains by biochemical, physiological, and molecular characterization . Bioscience Journal [online], vol. 36, no. 2, pp. 316–323. [Accessed28 May 2022]. DOI 10.14393/BJ-v36n2a2020-46256. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/46256.

Issue

Section

Agricultural Sciences