Mutational screening of the negative regulatory region of astrocyte elevated gene-1 promoter in hepatocellular carcinoma patients

Authors

  • Naghmeh Gholipour National Institute of Genetic Engineering and Biotechnology
  • Sakineh Mashjoor Hormozgan University
  • Zeinab Emruzi Tubkanlu University of Zabol
  • Hamideh Khajeh University of Zabol
  • Malihe Naderi Islamic Azad university
  • Nader Mansour Samaei Faculty of Medicine, Golestan University of Medical sciences
  • Gholamreza Motalleb University of Zabol

DOI:

https://doi.org/10.14393/BJ-v34n5a2018-42617

Keywords:

Astrocyte elevated gene -1, hepatocellular carcinoma, mutation, promoter, regulatory region

Abstract

Astrocyte elevated gene-1 (AEG-1) is a positive regulator of tumorigenesis in human cancer cells. Human AEG-1 gene is located in chromosome 8q22 having 12 exons/11 introns. Chromosome 8q22 is known to be a hot spot for genomic alterations in several cancerous cells involving HCC. The aim of the study was assess association between the negative regulatory region of AEG-1 promoter mutations and genetic susceptibility to hepatocellular carcinoma. The negative regulatory region of the human AEG-1 promoter was evaluated in a total of 50 Iranian hepatocellular carcinomas (HCC) patients. For investigating AEG-1 promoter polymorphisms the PCR-sequencing method was used. In this study was found two new mutation C>T (-633) and G>C (-660) in the patient group. But it was not revealed the statistically significant association between any mutations in this region of the AEG-1 promoter with HCC susceptibility. According to presented data, we can say that the negative regulatory region of the AEG-1 promoter mutations did not exihibit significant relevance with hepatocellular carcinoma. We recommend further studies on the efficacy of the AEG-1 promoter in therapeutic targeting of the HCC.

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Published

2018-10-11

How to Cite

GHOLIPOUR, N., MASHJOOR, S., TUBKANLU, Z.E., KHAJEH, H., NADERI, M., SAMAEI, N.M. and MOTALLEB, G., 2018. Mutational screening of the negative regulatory region of astrocyte elevated gene-1 promoter in hepatocellular carcinoma patients . Bioscience Journal [online], vol. 34, no. 5, pp. 1414–1421. [Accessed23 May 2022]. DOI 10.14393/BJ-v34n5a2018-42617. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/42617.

Issue

Section

Health Sciences