Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra

Authors

  • Yuanyuan Zhou North China University of Water Resources and Electric power
  • Song Li North China University of Water Resources and Electric power
  • Dalu Wang Zhengzhou University
  • Yaguang Zhao North China University of Water Resources and Electric power
  • Xiaolin Lei North China University of Water Resources and Electric power

DOI:

https://doi.org/10.14393/BJ-v34n3a2018-39998

Keywords:

type I collagen, inorganic acid, the triple helical structure, CD

Abstract

Usually, weak inorganic acids have been used to disperse collagen as green solvents for fabricating kinds of biomaterials all the time. However, it is an open question how much the dissolving process preserves or alters the native structure of collagen till now. Herein, we have examined the effect of three different solvents (HAc, HCl, H3PO4) on the secondary structures of collagen, based on circular dichroism (CD) spectra of collagen from 185 to 260 nm together with CDNN programs. We have found that collagen almost completely preserved its triple helical structure in the three inorganic acids at pH=3.0 or so, which demonstrated that it was the concentration of free H+ in the above three solutions whose pH was fixed at 3.0 that can maintain an proper amount of surface charge on the collagen colloidal particles and appropriately loose the three-helix structure, which can not only lead to a better dispersion behavior, but also maximize the preservation of the integrity of the collagen structure. Although the fractions of kinds of secondary structures in collagen were different from all the three solvents based on CDNN data, which gave very similar results for each other. These results was tested for the first time in this work to estimate the secondary structures for collagen in the different common inorganic acids, which provides a new avenue for green collagen solvents to prepare collagen-based composite with well triple-helical structure for tissue engineering.

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Published

2018-05-30

How to Cite

ZHOU, Y., LI, S., WANG, D., ZHAO, Y. and LEI, X., 2018. Estimation of type i collagen structure dissolved in inorganical acids from circular dichroism spectra . Bioscience Journal [online], vol. 34, no. 3, pp. 778–789. [Accessed4 December 2022]. DOI 10.14393/BJ-v34n3a2018-39998. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/39998.

Issue

Section

Biological Sciences