Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae)

Authors

  • Fernando Barnabe Cerqueira Universidade Federal do Tocantins
  • Giselly Batista Alves Universidade Federal do Tocantins
  • Roberto Franco Teixeira Corrêa Universidade de Brasilia
  • Soares Martins Embrapa Recursos Genéticos
  • Luiz Carlos Bertucci Barbosa Embrapa Recursos Genéticos
  • Ildon Rodrigues do Nascimento Universidade Federal do Tocantins
  • Rose Gomes Monnerat Universidade de Brasilia
  • Bergmann Morais Ribeiro Universidade Federal do Tocantins
  • Raimundo Wagner de Souza Aguiar Universidade Federal do Tocantins

DOI:

https://doi.org/10.14393/BJ-v32n6a2016-32741

Keywords:

Cry protein, Lepidopteran, Entomopathogen, Microbial insecticide, Insect control

Abstract

Spodoptera frugiperda (SMITH, 1797) (Lepidoptera: Noctuidae) affects diverse crops of great economic interest, for instance, it can cause severe yield losses in maize, rice and sorghum. In this study, a selection and characterization of Bacillus thuringiensis (BERLINER, 1911) isolates with a high insecticidal activity against S. frugiperda was performed. Fifty-two crystal-forming B. thuringiensis isolates that were identified from 3384 Bacillus-like colonies were examined and screened by PCR for the presence cry genes (cry1, cry1Aa, cry1Ab, cry1Ac, cry1D, cry2 and cry2Ab). Four isolates that showed high toxicity towards S. frugiperda were shown to harbor cry2 genes. The crystals were analyzed by electron microscopy and showed bipyramidal and cuboidal shapes. Furthermore, these four isolates had lethal concentration (LC50) values of 44.5 ng/cm2 (SUFT01), 74.0 ng/cm2 (SUFT02), 89.0 ng/cm2 (SUFT03) and 108 ng/cm2 (SUFT 04) to neonate S. frugiperda larvae. An ultrastructural analysis of midgut cells from S. frugiperda incubated with the SUFT01 spore-crystal complex showed disruptions in cellular integrity and in the microvilli of the midgut columnar cells. The isolates characterized in this work are good candidates for the control of S. frugiperda, and could  be used for the formulation of new bioinsecticides.

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Published

2016-12-06

How to Cite

CERQUEIRA, F.B., ALVES, G.B., CORRÊA, R.F.T., MARTINS, .S., BARBOSA, L.C.B., DO NASCIMENTO, I.R., MONNERAT, R.G., RIBEIRO, B.M. and AGUIAR, R.W. de S., 2016. Selection and characterization of Bacillus thuringiensis isolates with a high insecticidal activity against Spodoptera frugiperda (Lepidoptera: Noctuidae) . Bioscience Journal [online], vol. 32, no. 6, pp. 1522–1536. [Accessed21 May 2022]. DOI 10.14393/BJ-v32n6a2016-32741. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/32741.

Issue

Section

Agricultural Sciences