Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae)


  • Ana Claudia Oliveira Firak Cosmoski Universidade Católica Dom Bosco
  • Antonia Railda Roel Universidade Católica Dom Bosco
  • Karla Rejane de Andrade Porto Universidade católica Dom Bosco
  • Rosemary Matias Coelho Universidade Anhanguera
  • Michel Robin Honer Universidade Católica Dom Bosco
  • Priscila Rezende Motti universidade cataólica Dom Bosco



vector control, plant active ingredients and plant insecticides.


In the search for alternative ways to control Aedes aegypti with minimal environmental impact and in a manner that preserves human health, this study sought to evaluate the larvicidal effect of the invasive and antioxidant Spermacoce latifolia plant by performing a phytochemical study. Phytochemical screenings were done according to characterization reactions and thin layer chromatography. Phenolics compounds content (Folin-Ciocalteu's) and flavonoids (AlCl3) spectrophotometric was performed, and the antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical results revealed the presence of phenolic, flavonoid, tannin, steroid, free triterpene, coumarin, and alkaloid compounds. The content of total phenols (TPs) (482.7 ± 1.8 mg mgGA g-1) and flavonoids (165.4 ± 1.5 mg QE g-1) accounted for the antioxidant activity of 150 µg.mL-1 methanolic extract. In the proposed bioassays, groups of 25 third-stage larvae were challenged at different concentrations of plant crude extract (1.0, 0.5, 0.25, and 0.1 g L-1) of weight per volume in four replicates. In multiple concentration tests, the concentrations were selected to range from 0% to 100% mortality after 24 hours of contact with the solution. Toxicity was defined as the inhibition or total inactivity of the larvae. It was concluded that the methanol extract had an LC50 of 0.62 g L-1, indicating its potential use as a larvicide against A. aegypti and linking its activity to its phenolic and flavonoid components.


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Author Biographies

Karla Rejane de Andrade Porto, Universidade católica Dom Bosco

Possui graduação em Nutrição pela Universidade Federal de Alagoas (1995) e mestrado em Química e Biotecnologia pela Universidade Federal de Alagoas (2003). Tem experiência na área de Nutrição, com ênfase em Bioquímica da Nutrição, atuando principalmente nos seguintes temas: aedes e testes inseticidas, toxicidade, artemia e screening, cajuzinho do cerrado e tecnologia de alimentos. Atualmente é DTI 1 do Instituto Nacional de Areas Umidas

Priscila Rezende Motti, universidade cataólica Dom Bosco

Bióloga, formada pela Universidade Católica Dom Bosco, foi bolsista durante toda a graduação do PIBIC também do CNPq pelo projeto Screennig de plantas do Cerrado com potencial fitotóxico e larvidacida sobre Aedes aegypti. Atualmente é Bolsista por excelência UCDB, Mestranda em Biotecnologia na área da saúde, produção de produtos naturais e sintéticos com princípios ativos larvicida para Aedes aegypti.




How to Cite

COSMOSKI, A.C.O.F., ROEL, A.R., PORTO, K.R. de A., COELHO, R.M., HONER, M.R. and MOTTI, P.R., 2015. Phytochemistry and larvicidal activity of Spermacoce latifolia Aubl. (Rubiaceae) in the control of Aedes aegypti L. (Culicidae) . Bioscience Journal [online], vol. 31, no. 5, pp. Agricultural Sciences. [Accessed25 May 2022]. DOI 10.14393/BJ-v31n5a2015-26333. Available from:



Biological Sciences