Absence of detection of Xanthomonas axonopodis Pv. Malvacearum in cotton seeds used in the northeast region of Mato Grosso do Sul in a three-based crop season study

Authors

  • Gustavo de Faria Theodoro Universidade Federal de Mato Grosso do Sul
  • Hectory de Castro Correia Universidade Federal de Mato Grosso do Sul
  • Rafael Rodrigues da Silva Universidade Federal de Mato Grosso do Sul
  • André Augusto Chumpati Universidade Federal de Mato Grosso do Sul
  • Josiane Vogel Cortina Universidade Federal de Mato Grosso do Sul
  • Antonio Carlos Maringoni Universidade Estadual Paulista Júlio de Mesquita Filho

Abstract

The aim of this work was to evaluate the presence of the causal agent of angular leaf spot, Xanthomonas axonopodis pv. malvacearum, in 38 cotton seeds samples with or without linter used in the northeast region of Mato Grosso do Sul, Brazil, during the crop seasons of 2008/09, 2009/10 e 2010/11. It was used the semi selective culture media MSSXAN (beef extract - 3 g, peptone - 5 g, soluble starch - 10 g, sucrose - 5g; Tween 80 - 10 mL; CaCl2 - 0.25 g; crystal violet solution at 1% - 150 mL; cephalexin - 50 mg; chlorothalonil - 10 mg; methyl thyophanate - 10 mg; agar - 15 g; sterile distilled water - 1000 ml) for the isolation of the bacteria and the suspect colonies of being X. axonopodis pv. malvacearum were purified and submitted to the Gram stain, potassium hydroxide and pathogenicity tests in the leaves of cotton plants of the susceptible genotype NC 53345. The presence of X. a. pv. malvacearum was not detected in the evaluated seed samples.

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Published

2014-02-20

How to Cite

THEODORO, G. de F., CORREIA, H. de C., SILVA, R.R. da, CHUMPATI, A.A., CORTINA, J.V. and MARINGONI, A.C., 2014. Absence of detection of Xanthomonas axonopodis Pv. Malvacearum in cotton seeds used in the northeast region of Mato Grosso do Sul in a three-based crop season study . Bioscience Journal [online], vol. 30, no. 2, pp. 326–331. [Accessed23 February 2024]. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/17975.

Issue

Section

Agricultural Sciences