Absence of detection of Xanthomonas axonopodis Pv. Malvacearum in cotton seeds used in the northeast region of Mato Grosso do Sul in a three-based crop season study

Authors

  • Gustavo de Faria Theodoro UFMS - Campus de Chapadão do Sul
  • Hectory de Castro Correia UFMS
  • Rafael Rodrigues da Silva UFMS
  • André Augusto Chumpati UFMS
  • Josiane Vogel Cortina UFMS
  • Antonio Carlos Maringoni UNESP

Keywords:

Gossypium hirsutum, seed pathology, dissemination

Abstract

The aim of this work was to evaluate the presence of the causal agent of angular leaf spot, Xanthomonas axonopodis pv. malvacearum, in 38 cotton seeds samples with or without linter used in the northeast region of Mato Grosso do Sul, Brazil, during the crop seasons of 2008/09, 2009/10 e 2010/11. It was used the semi selective culture media MSSXAN (beef extract - 3 g, peptone - 5 g, soluble starch - 10 g, sucrose - 5g; Tween 80 - 10 mL; CaCl2 - 0.25 g; crystal violet solution at 1% - 150 mL; cephalexin - 50 mg; chlorothalonil - 10 mg; methyl thyophanate - 10 mg; agar - 15 g; sterile distilled water - 1000 ml) for the isolation of the bacteria and the suspect colonies of being X. axonopodis pv. malvacearum were purified and submitted to the Gram stain, potassium hydroxide and pathogenicity tests in the leaves of cotton plants of the susceptible genotype NC 53345. The presence of X. a. pv. malvacearum was not detected in the evaluated seed samples.

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Author Biography

Gustavo de Faria Theodoro, UFMS - Campus de Chapadão do Sul

Professor Adjunto, Nível III Laboratório de Fitopatologia Campus de Chapadão do Sul Universidade Federal de Mato Grosso do Sul

Published

2013-11-20

How to Cite

THEODORO, G. de F., CORREIA, H. de C., DA SILVA, R.R., CHUMPATI, A.A., CORTINA, J.V. and MARINGONI, A.C., 2013. Absence of detection of Xanthomonas axonopodis Pv. Malvacearum in cotton seeds used in the northeast region of Mato Grosso do Sul in a three-based crop season study . Bioscience Journal [online], vol. 30, no. 2. [Accessed21 May 2022]. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/17975.

Issue

Section

Agricultural Sciences