Epiphytic and endophytic bacteria for the control of Botrytis cinerea in vitro and in grape berries of cv. sauvignon blanc in southern Brazil
DOI:
https://doi.org/10.14393/BJ-v41n0a2025-74859Palavras-chave:
EnglishResumo
A podridão cinzenta (PC) em videiras, causada pelo Botrytis cinerea, é uma doença de pré e pós-colheita que causa perdas significativas no sul do Brasil. O objetivo deste estudo foi avaliar o potencial de bactérias epifíticas e endofíticas no controle do B. cinerea in vitro e in vivo na variedade 'Sauvignon Blanc' (SB). Os isolados de B. cinerea e bactérias epifíticas e endofíticas foram obtidos de cachos de uva e do filoplano da variedade SB, respectivamente. Quatro isolados epifíticos e dois endofíticos que mostraram os maiores índices de antibiose in vitro e in vivo foram identificados usando fenotipagem e sequenciamento de DNA. Os tratamentos foram realizados em um delineamento experimental completamente casualizado com cinco repetições, incluindo: a) controle; b) B. cinerea; c) produto comercial - Ecoshot® com Bacillus amyloliquefaciens; d) bactérias epifíticas e e) bactérias endofíticas. Entre os 52 isolados, quatro epifíticos e dois endofíticos, apresentaram os maiores grau de inibição e inibiram significativamente o crescimento do B. cinerea. Os isolados UEP40, UEP43, UEN13 e UEN14 apresentaram reações positivas para ureia e indol. Os isolados epifíticos UEP43 e UEP51 e o isolado endofítico UEN13 reduziram a severidade da BBR em bagas de uva destacadas em aproximadamente 55% e 49% entre 72 e 168 horas após a inoculação, respectivamente. Esses isolados foram identificados como Serratia marcescens, Curtobacterium flaccumfaciens pv. flaccumfaciens e Staphylococcus equorum, respectivamente. Esses isolados bacterianos são agentes potenciais de biocontrole de B. cinerea. No entanto, mais pesquisas são necessárias para confirmar sua eficácia em vinhedos no sul do Brasil.
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Copyright (c) 2025 Claudemar Helmuth Herpich, Leocir José Welter, Glória Regina Botelho, Fábio Nascimento da Silva, Ricardo Trezzi Casa, Amauri Bogo
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
