An efficient protocol for in vitro regeneration of peanut (Arachis hypogaea L.) cultivar L14

Phung Thi Bich  Hoa; Nguyen Hoang  Tue; Phan Thi Quyen  Trang; Le Thi  Hang; Nguyen Quang Duc  Tien; Nguyen Hoang  Loc

doi:10.14393/BJ-v37n0a2021-56949

Autores

Phung Thi Bich Hoa Hue University https://orcid.org/0000-0002-9989-7713
Nguyen Hoang Tue Hue University https://orcid.org/0000-0003-4387-0201
Phan Thi Quyen Trang Hue University https://orcid.org/0000-0001-5699-3169
Le Thi Hang Hue University https://orcid.org/0000-0002-9570-3547
Nguyen Quang Duc Tien Hue University https://orcid.org/0000-0002-7330-3139
Nguyen Hoang Loc Hue University https://orcid.org/0000-0002-6387-0359

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-56949

Palavras-chave:

Arachis hypogaea, In Vitro Propagation, Rooting, Shoot Multiplication, Shoot Regeneration.

Resumo

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.

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Referências

AL-JOBOURY, K.H.R. In vitro propagation of groundnut (Arachis hypogaea L.). Ibn Al-Haitham Journal for Pure and Applied Science. 2012, 3(2), 13-19.

CHENG, M., HIS, D.C.H. and PHILLIPS, G.C. In vitro regeneration of Valencia-type peanut (Arachis hypogaea L.) from cultured petioles, epicotyl sections, and other seedling explants. Peanut Science. 1992, 19(2), 82-87. https://doi.org/10.3146/i0095-3679-19-2-5

CID, L.P.B., et al. Plant regeneration from seedling explants of Eucalyptus grandis x E. urophylla. Plant Cell, Tissue and Organ Culture. 1999, 56(1), 17-23. https://doi.org/10.1023/A:1006283816625

CUONG, L.N., NHUNG, N.T. and DIEM, N.T. Fungal inhibition and disease control of groundnut stem rot (Sclerotium rolfsii) by nanosilver liquid. Hue University Journal of Science: Agriculture and Rural Development. 2018, 127(3A), 161-171.

DANG, P.M., et al. Identification of expressed R-genes associated with leaf spot diseases in cultivated peanut. Molecular Biology Reports. 2019, 46(1), 225-239. https://doi.org/10.1007/s11033-018-4464-5

IQBAL, M.M., et al. In vitro micropropagation of peanut (Arachis hypogaea) through direct somatic embryogenesis and callus culture. International Journal of Agriculture & Biology. 2011, 13(5), 811-814.

JAGADEESH, B., et al. Amenability of the sugarcane variety 2005 T 16 to shoot tip culture. Journal of Sugarcane Research, 2011, 1(2), 75-77.

LIMBUA, P.G., NGUGI, M.P. and ODUOR, R.O. In vitro regeneration protocol of Kenyan adapted groundnut (Arachis hypogaea L.) genotypes using cotyledonary node explants. Journal of Plant Biochemistry & Physiology. 2019, 7(1), 1000233. https://doi.org/10.4172/2329-9029.1000233

MAINA, S.M., et al. Surface sterilant effect on the regeneration efficiency from cotyledon explants of groundnut (Arachis hypogaea L.) varieties adapted to eastern and Southern Africa. African Journal of Biotechnology. 2010, 9(20), 2866-2871.

MARTINS, J.P.R., et al. Effect of synthetic auxins on in vitro and ex vitro bromeliad rooting. Pesquisa Agropecuária Tropical. 2013, 43(2), 138-146. https://doi.org/10.1590/S1983-40632013000200009

MURASHIGE, T. and SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum. 1962, 15(3), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

NAGESHWARA-RAO, R.C. and NIGAM, S.N., 2001. Genetic options for drought management for groundnut. In: SAXENA, N.P., ed. Management of agricultural drought: Agronomic and Genetic Options, New Delhi: Oxford & IBH Publishing Co. Pvt. Ltd., pp. 123-141.

OZUDOGRU, E.A., OZDEN-TOKATLI, Y. and AKCIN, A. Effect of silver nitrate on multiple shoot formation of Virginia-type peanut through shoot tip culture. In Vitro Cellular & Developmental Biology-Plant. 2005, 41(2), 151-156. https://doi.org/10.1079/IVP2004591

ÖZKAN, H. and AASIM, M. Potential of pretreated explants of peanut (Arachis hypogaea Linn.) to micropropagation under in vitro conditions. Pakistan Journal of Agricultural Sciences. 2019, 56(3), 775-780. https://doi.org/10.21162/PAKJAS/19.8262

PALANIVEL, S. and JAYABALAN, N. Direct multiple shoot induction from different mature seed explants of groundnut (Arachis hypogaea L.). Philippine Journal of Science. 2002, 131(2), 127-135.

PALANIVEL, S., et al. Arachis hypogaea L. direct organogenesis and SDS-PAGE analysis in groundnut. British Biotechnology Journal. 2014, 4(6), 720-732. https://doi.org/10.9734/BBJ/2014/9237

PEETERS, A.J.M., et al. In vitro flower bud formation in tobacco: Interaction of hormones. Plant Physiology. 1991, 97(1), 402-408. https://doi.org/10.1104/pp.97.1.402

PESTANA, M.C., et al. In vitro regeneration of peanut (Arachis hypogaea L.) through organogenesis: Effect of culture temperature and silver nitrate. In Vitro Cellular & Developmental Biology-Plant. 1999, 35(3), 214-216. https://doi.org/10.1007/s11627-999-0080-3

PRATAP, A., et al. Potential, constraints, and applications of in vitro methods in improving grain legumes. Plant Breeding. 2018, 137(3), 235-249. https://doi.org/10.1111/pbr.12590

RADHAKRISHNAN, T., et al. Micropropagation in peanut (Arachis hypogaea L.). Biologia Plantarum. 2000, 43(3), 447-450. https://doi.org/10.1023/A:1026743822546

RASOOL, R., et al. Effect of BAP and NAA on shoot regeneration in Prunella vulgaris. Journal of Natural Sciences and Mathematics, Qassim University. 2009, 3(1), 21-26. ISSN 1658-4031.

SAVAGE, G.P. and KEENAN, J.I., 1994. The composition and nutritive value of groundnut kernels. In: J. SMARTT, ed., The groundnut crop: A scientific basis for improvement, Dordrecht:Springer, pp. 173-213.

SHARMA, K.K. and BHATNAGAR-MATHUR, P., 2006. Peanut (Arachis hypogaea L.). In: WANG, K., ed., Methods in molecular biology, vol. 343: Agrobacterium Protocols, 2/e, volume 1. New Jersey:Humana Press Inc., pp. 347-358.

SUU, T.D., et al. Peanut Planting and Care Techniques. Ha Noi:Vietnam Academy of Agricultural Sciences - National Agricultural Extension Center, 2017.

TIWARI, S. and TULI, R. Factors promoting efficient in vitro regeneration from de-embryonated cotyledon explants of Arachis hypogaea L. Plant Cell, Tissue and Organ Culture. 2008, 92(1), 15-24. https://doi.org/10.1007/s11240-007-9297-1

TORRES, K.C. Tissue Culture Techniques for Horticultural Crops. New York:Van Nostrand Reinhold, 1989.

VASAVIRAMA, K. and KIRTI, P.B. Increased resistance to late leaf spot disease in transgenic peanut using a combination of PR genes. Functional & Integrative Genomics. 2012, 12(4), 625-634. https://doi.org/10.1007/s10142-012-0298-8

VENKATACHALAM, P., et al. Induction of direct somatic embryogenesis and plant regeneration from mature cotyledon explants of Arachis hypogaea L. Current Science. 1999, 77(2), 269-273.

VERMA, A., et al. Effects of in vitro triacontanol on growth, antioxidant enzymes, and photosynthetic characteristics in Arachis hypogaea L. Brazilian Journal of Plant Physiology. 2011, 23(4), 271-277. https://doi.org/10.1590/S1677-04202011000400004

VERMA, A., MALIK, C.P. and GUPTA, V.K. Sodium nitroprusside-mediated modulation of growth and antioxidant defense in the in vitro raised plantlets of peanut genotypes. Peanut Science. 2014, 41(1), 25-31. https://doi.org/10.3146/PS12-13.1