Sex-related variations in the morphological structure of puma (Puma concolor Linnaeus, 1771) ear skin

Gabriela Pereira de Oliveira Lira; Leonardo Vitorino Costa de Aquino; Samara Lima Olindo; Matheus Barbosa do Nascimento; Herlon Victor Rodrigues Silva; Alexsandra Fernandes Pereira

doi:10.14393/BJ-v40n0a2024-71164

Authors

Gabriela Pereira de Oliveira Lira Universidade Federal Rural do Semi-Árido
Leonardo Vitorino Costa de Aquino Universidade Federal Rural do Semi-Árido
Samara Lima Olindo Universidade Federal Rural do Semi-Árido https://orcid.org/0009-0000-0435-7881
Matheus Barbosa do Nascimento Universidade Federal Rural do Semi-Árido https://orcid.org/0000-0003-2661-0846
Herlon Victor Rodrigues Silva Universidade Federal do Ceará
Alexsandra Fernandes Pereira Universidade Federal Rural do Semi-Árido https://orcid.org/0000-0003-2137-854X

DOI:

https://doi.org/10.14393/BJ-v40n0a2024-71164

Keywords:

Biobanks, Conservation strategies, Histomorphometry, Wild felids

Abstract

The ear tissue of vulnerable felines, such as the puma, holds potential biological material for creating biobanks. However, skin composition can differ significantly between individuals of the of the same species and even between sexes, based on different environments. Therefore, identifying morphological similarities across these populations is crucial for developing accurate protocols. This study aimed to characterize and evaluate the structure and composition of ear skin in both a male and a female puma using histological techniques. Histomorphometric analysis revealed a total thickness of 304.65 µm and 238.95 µm for the male and the female, respectively. The epidermis was notably thinner in the female compared to the male. Specifically, the thickness of the basal, spinous, and corneum layers in the female was 2.62 µm, 10.07 µm, and 3.15 µm, respectively, compared to 3.49 µm, 13.94 µm, and 3.66 µm in the male, respectively. Melanocytes, keratinocytes, and fibroblasts totaled 26, 24, and 50 cells in the male and 21, 25, and 54 cells in the female. Moreover, chondrocytes (male: n=33 and female: n=41) and perichondrium (male=13.99 µm and female=9.05 µm) were observed in the cartilage. These results demonstrate the histomorphometric differences and similarities between a male and a female puma, consistent with observation in other felines. This information is relevant for a targeted approach for establishing biobanks for this species.

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References

AFFOLTER, V. K., and MOORE, P. F. Histologic features of normal canine and feline skin. Clinics in Dermatology. 1994, 12, 491-497. https://doi.org/10.1016/0738-081X(94)90215-1

ARAÚJO, I. C. F., et al. Implementation of cognitive and food activities on jaguars (Panthera onca) and puma’s (Puma concolor) routine kept in captivity. Brazilian Journal of Animal and Environmental Research. 2019, 2, 713-720.

AZEVEDO, F. C., et al. Avaliação do risco de extinção da onça-parda, Puma concolor (Linnaeus, 735 1771) no Brasil. Biodiversidade Brasileira. 2013, 3, 107-21. https://doi.org/10.37002/biodiversidadebrasileira.v3i1.377

AZZI, L., et al. Gender differences in mouse skin morphology and specific effects of sex steroids and dehydroepiandrosterone. Journal of Investigative Dermatology. 2005, 124(1), 22-27. https://doi.org/10.1111/j.0022-202X.2004.23545.x

BARONE, M. A., et al. Gonadotrophin dose and timing of anaesthesia for laparoscopic artificial insemination in the puma (Felis concolor). Reproduction. 1994, 101(1), 103-108. https://doi.org/10.1530/jrf.0.1010103

BORER, E. T., et al. What determines the strength of a trophic cascade? Ecology. 2005, 86, 528-537. https://doi.org/10.1890/03-0816

BORGES, A. A., et al. Conservation of somatic tissue derived from collared peccaries (Pecari tajacu Linnaeus, 1758) using direct or solid-surface vitrification techniques. Cytotechnology. 2017, 69, 643-654. https://doi.org/10.1007/s10616-017-0074-7

CICHOREK, M., et al. Skin melanocytes: biology and development. Advances in Dermatology and Allergology. 2013, 30, 30-41. https://doi.org/10.5114/pdia.2013.33376

CULVER, M. 2009. Lessons and insights from evolution, taxonomy, and conservation genetics. In: M. HORNOCKER and S. NEGRI, eds. Cougar: ecology and conservation. University of Chicago Press, Chicago, pp. 27-40.

CURRIER, M. J. P. Felis concolor. Mammalian species. 1983, 200, 1-7. https://doi.org/10.2307/3503951

DECO-SOUZA, T. D., et al. Comparison between two glycerol concentrations to cryopreservation of semen of mountain lions (Puma concolor). Pesquisa Veterinária Brasileira. 2013, 33, 512-516. https://doi.org/10.1590/S0100-736X2013000400015

ECHEVERRY, D. M., et al. Characterization of mesenchymal stem cells derived from adipose tissue of a cougar (Puma concolor). Animal Reproduction. 2020, 17(2), e20190109. https://doi.org/10.1590/1984-3143-ar2019-0109

ELLIOTT, G. D., WANG, S., and FULLER, B. J. Cryoprotectants: A review of the actions and applications of cryoprotective solutes that modulate cell recovery from ultra-low temperatures. Cryobiology. 2017, 76, 74-91. https://doi.org/10.1016/j.cryobiol.2017.04.004

ESTEVES, M. L. D. A. B., and BRANDÃO, B. J. F. Collagen and Skin Aging. BWS Journal. 2022, 5, 1-10.

GRABAU, J. H., et al. Comparison of anatomical characteristics of the skin for several laboratory animals. Final Report, USAF Amstrong Laboratories, Wright-Patterson AFB, OH. 1995.

GUAN, W. J., et al. Establishment and cryopreservation of a fibroblast cell line derived from Bengal tiger (Panthera tigris tigris). Cryo-Letter. 2010, 31(2), 130-138.

HAAG, M., et al. Age and skin site related differences in steroid metabolism in male skin point to a key role of sebocytes in cutaneous hormone metabolism. Dermato-Endocrinology. 2012, 4, 58-64. https://doi.org/10.4161/derm.19201

HALL, G., and PHILLIPS, T. J. Estrogen and skin: the effects of estrogen, menopause, and hormone replacement therapy on the skin. Journal of the American Academy of Dermatology. 2005, 53, 555-568. https://doi.org/10.1016/j.jaad.2004.08.039

ISOLA, J. G. M. P., et al. Morphology, ultrastructure and morphometry of the tegument of paca (Cuniculus paca Linnaeus, 1766) raised in captivity. Brazilian Veterinary Research. 2013. 33, 674-682. https://doi.org/10.1590/S0100-736X2013000500020

KHAVKIN, J., and ELLIS, D. A. F. Aging Skin: Histology, Physiology, and Pathology. Facial Plastic Surgery Clinics of North America. 2011, 19, 229-234. https://doi.org/10.1016/j.fsc.2011.04.003

LABARGE, L. R., et al. Pumas Puma concolor as ecological brokers: a review of their biotic relationships. Mammal Review. 2022, 52(3), 360-376. http://dx.doi.org/10.1111/mam.12281

LANE, M. E. Skin penetration enhancers. International Journal of Pharmaceutics. 2013, 447, 12-21. https://doi.org/10.1016/j.ijpharm.2013.02.040

LAUNDRÉ, J. W., and HERNÁNDEZ, L. 2010. What we know about pumas in Latin America. In: M. HORNOCKER and S. NEGRI, eds. Cougar: Ecology and Conservation. University of Chicago Press, Chicago, pp. 76-90.

LEON-QUINTO, T., et al. Developing biological resource banks as a supporting tool for wildlife reproduction and conservation. The Iberian lynx bank as a model for other endangered species. Animal Reproduction Science. 2009, 112, 347-361. https://doi.org/10.1016/j.anireprosci.2008.05.070

LIRA, G. P. O., et al. Effects of somatic tissue cryopreservation on puma (Puma concolor L, 1771) tissue integrity and cell preservation after in vitro culture. Cryobiology. 2021, 101, 52-60. https://doi.org/10.1016/j.cryobiol.2021.06.003

LIRA, G. P. O., et al. Morphological, ultrastructural, and immunocytochemical characterization and assessment of puma (Puma concolor Linnaeus, 1771) cell lines after extended culture and cryopreservation. Biopreservation and Biobanking. 2022, 20(6), 557-566. https://doi.org/10.1089/bio.2021.0117

MARIO, L. C., et al. Morphology of the Oligoryzomys nigripes respiratory system. Anatomia, Hhistologia, Embryologia. 2018, 47(4), 364-371. https://doi.org/10.1111/ahe.12363

MARKOVA, M. S., et al. A role for the androgen receptor in collagen content of the skin. Journal of Investigative Dermatology. 2004, 123, 1052-1056. https://doi.org/10.1111/j.0022-202X.2004.23494.x

MEYER, W., and NEURAND, K. A comparative scanning electron microscopic view of the integument of domestic mammals. Scanning Microscopy. 1987, 1, 169-180.

MILLER, A. M., et al. Oocyte recovery, maturation and fertilization in vitro in the puma (Felis concolor). Theriogenology. 1989, 31(1), 228. https://doi.org/10.1530/jrf.0.0880249

MOSER, K., et al. Passive skin penetration enhancement and its quantification in vitro. European Journal of Pharmaceutics and Biopharmaceutics. 2001, 52, 103-112. https://doi.org/10.1016/S0939-6411(01)00166-7

MOULAVI, F., et al. Interspecies somatic cell nuclear transfer in Asiatic cheetah using nuclei derived from post-mortem frozen tissue in absence of cryo-protectant and in vitro matured domestic cat oocytes. Theriogenology. 2017, 90, 197-203. https://doi.org/10.1016/j.theriogenology.2016.11.023

NIELSEN, C., et al. Puma concolor (errata version published in 2016). The IUCN Red List of Threatened Species 2015, e.T18868A97216466, 2015.

OLIVEIRA, T. G. 1994. Jaguar. In: T.G. OLIVEIRA, eds. Neotropical cats: ecology and conservation. São Luís, MA, EDUFMA. pp. 244.

PRAXEDES, É. A., et al. Quantitative and descriptive histological aspects of jaguar (Panthera onca Linnaeus, 1758) ear skin as a step towards formation of biobanks. Anatomia, Histologia y Embriologia. 2020. 49, 121-129. https://doi.org/10.1111/ahe.12500

PRAXEDES, É. A., et al. Effects of cryopreservation techniques on the preservation of ear skin – An alternative approach to conservation of jaguar, Panthera onca (Linnaeus, 1758). Cryobiology. 2019, 88, 15-22. https://doi.org/10.1016/j.cryobiol.2019.04.007

THORNTON, M. J. The biological actions of estrogens on skin. Experimental Dermatology. 2002, 11, 487-502. https://doi.org/10.1034/j.1600-0625.2002.110601.x

SALEHI, M., LAVVAF, A., and FARAHVASH, T. Skin quality and physical properties of leather based on sex, age and body parts of goats reared on sub‐humid hill country. Iranian Journal of Applied Animal Science. 2013, 3, 853-857.

SANTOS, F., et al. Prey availability and temporal partitioning modulate felid coexistence in Neotropical forests. Plos One. 2019, 14, e0213671. https://doi.org/10.1371/journal.pone.0213671

SANTOS, M. D. C. B., et al. Evaluation of different skin regions derived from a postmortem jaguar, Panthera onca (Linnaeus, 1758), after vitrification for development of cryobanks from captive animals. Zoo Biology. 2021, 1-8. https://doi.org/10.1002/zoo.21605

SULAIMON, S. S., and KITCHELL, B. E. The biology of melanocytes. Veterinary Dermatology. 2003, 14, 57-65. https://doi.org/10.1046/j.1365-3164.2003.00327.x

VIANA, J. V. S., et al. Effects of vitrification techniques on the formation of skin cryobank of the ocelot (Leopardus pardalis). Cryo-Letter. 2023, 47, 47-56. https://doi.org/10.54680/fr23110110412

ZANIN, M., et al. What should I eat: feeding behaviour of puma in a Brazilian protected semi-arid area. Hystrix, the Italian Journal of Mammalogy, 2020. http://dx.doi.org/10.4404/hystrix–00265-2019