Spray drying of cellulases produced by Bacillus sp. SMIA-2

Erica  Cruz; Simone Vilela Talma; João Batista Barbosa; Raphael Pires Bolzan; Silvania Alves Ladeira; Raquel Vieira de Carvalho; Meire Lelis Leal Martins

doi:10.14393/BJ-v40n0a2024-64855

Authors

Erica Cruz Universidade Estadual do Norte Fluminense https://orcid.org/0000-0002-5264-6116
Simone Vilela Talma Instituto Federal de Sergipe https://orcid.org/0000-0003-3416-9202
João Batista Barbosa Instituto Federal de Sergipe https://orcid.org/0000-0002-8608-254X
Raphael Pires Bolzan Instituto Federal do Espírito Santo https://orcid.org/0000-0002-3269-5180
Silvania Alves Ladeira Instituto Federal de Alagoas
Raquel Vieira de Carvalho Universidade Federal do Espírito Santo https://orcid.org/0000-0001-7329-9750
Meire Lelis Leal Martins Universidade Estadual do Norte Fluminense https://orcid.org/0000-0002-9812-0232

DOI:

https://doi.org/10.14393/BJ-v40n0a2024-64855

Keywords:

Additives, Avicelase, Bacillus, Carboxymethylcellulase, Spray drying.

Abstract

Microbial cellulases present biotechnological potential to be used in several industries, including food, brewery and wine, industrial waste for chemical feedstock, animal feed, pulp and paper, agriculture, textile and detergent production. In this work, cellulases produced by the thermophilic Bacillus sp. SMIA-2 in submerged cultures containing sugarcane bagasse, corn steep liquor and passion fruit rind flour were spray-dried, in an attempt to improve their stability for industrial purposes. The cellulases were spray dried and analyzed before and after the drying process and subsequent storage. A Central Composite Design (CCD) 2³ was used to investigate the effect of different concentrations of arabic gum and microcrystalline cellulose, as well as the spray dryer inlet temperature on the cellulase spray drying process. The results evidenced that the combination of 1.0 % (w/v) arabic gum and 1.0% (w/v) microcrystalline cellulose, at inlet temperature of 70 °C, was effective in maintaining the activities of both avicelases (avicel-hydrolyzing enzymes) and carboxymethylcellulases (carboxymethylcellulose-hydrolyzing enzymes - CMCase). The dried avicelase was completely preserved when stored at 5^oC, while the CMCase retained 89% of its activity, which indicates promising potential for industrial uses, especially in detergent formulations.

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