Antioxidant activity of polysaccharides extracted from Lentinus edodes mycelia and their protective effects on INS-1 cells

Authors

  • Jianli Liu Liaoning University
  • Weiyu Wang Liaoning University
  • Hui Yu Liaoning University
  • Xiangyu Cao Liaoning University
  • Ying Wang Soil and Fertilizer Station of Liaoning Province
  • Meijia Liu Liaoning University
  • Xiao Yan Liaoning University
  • Hongsheng Liu Research Center for Computer Simulating and Information Processing of Bio-macromolecules of Liaoning Province

DOI:

https://doi.org/10.14393/BJ-v32n1a2016-33809

Keywords:

Lentinus edodes mycelia, polysaccharide, antioxidant, oxidative stress

Abstract

The disruption of the delicate balance between the generation of reactive oxygen species (ROS) and antioxidant scavenging systems can lead to many health problems. Polysaccharides extracted from fungi fruit body and myceliums have several potential health benefits. In this study, the antioxidant capacity and cell protective effects of a polysaccharide isolated from Lentinus edodes mycelia (LMP) were investigated. The antioxidant properties of LMP were screened using radical scavenging (1, 1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, and superoxide anion), reducing power and inhibition of lipid peroxidation assays. Intracellular reactive oxygen species (ROS), in response to H2O2, was determined by the dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay. Rat insulinoma (INS-1) cells apoptosis was detected using flow cytometry analysis. The results showed that LMP exhibited significantly strong radical scavenging activity. At the concentration of 2.0 mg mL-1, DPPH radical, hydroxyl radical, and superoxide anion radical scavenging rates are 80.32 ± 2.58%, 92.56 ± 3.11% and 93.73 ± 2.82%, respectively. The reducing power and inhibition of lipid peroxidation activity of LMP were as strong as the positive control. LMP alleviated the intracellular reactive oxygen species level and inhibited islet cell apoptosis significantly. The results open perspectives for studies of LMP in the treatment of diabetes.

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Published

2016-12-06

How to Cite

LIU, J., WANG, W., YU, H., CAO, X., WANG, Y., LIU, M., YAN, X. and LIU, H., 2016. Antioxidant activity of polysaccharides extracted from Lentinus edodes mycelia and their protective effects on INS-1 cells . Bioscience Journal [online], vol. 32, no. 6, pp. 1679–1688. [Accessed22 November 2024]. DOI 10.14393/BJ-v32n1a2016-33809. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/33809.

Issue

Section

Health Sciences