Effectiveness of Azospirillum brasilense and Pseudomonas fluorescens in sugarcane development

Lovely Santia Edouard; Anderson Alves dos Santos; Emanuele Storer Dias; Larissa de Assis Carretts; Fabiana Gisele da Silva Pinto; Leopoldo Sussumu  Matsumoto

doi:10.14393/BJ-v42n0a2026-77247

Authors

Lovely Santia Edouard Universidade Estadual do Norte do Paraná https://orcid.org/0009-0009-1574-4735
Anderson Alves dos Santos Universidade Estadual do Norte do Paraná https://orcid.org/0009-0001-3050-5750
Emanuele Storer Dias Universidade Estadual do Norte do Paraná https://orcid.org/0009-0001-3313-1133
Larissa de Assis Carretts Western Paraná State University
Fabiana Gisele da Silva Pinto Western Paraná State University https://orcid.org/0000-0002-0486-8486
Leopoldo Sussumu Matsumoto Western Paraná State University

DOI:

https://doi.org/10.14393/BJ-v42n0a2026-77247

Keywords:

Bio-inputs, Bioreactor, PGPR, Rhizobacteria, Sustainable agriculture.

Abstract

The use of bio-inputs has been highly effective in cultivating large crops. Azospirillum brasilense and Pseudomonas fluorescens are referred to as plant growth-promoting rhizobacteria (PGPR), which act through both direct mechanisms, providing nutrients, and indirect mechanisms, such as biocontrol. This study aimed to evaluate the effectiveness of bio- inputs based on A. brasilense and P. fluorescens in sugarcane development. These bio-inputs were produced in bioreactors in isolated and combined fermentation. The test was conducted in a plant growth chamber with controlled temperature, photoperiod, and humidity. Pots received a sugarcane bud, and the bio-inputs were applied. The treatments consisted of T1 = control, T2 = A. brasilense, T3 = P. fluorescens, T4 = A. brasilense + P. fluorescens (combined fermentation) 0 hours (shelf life), and T5 = A. brasilense + P. fluorescens (combined fermentation) 180 hours (shelf life). The biocontrol action of bio-inputs against phytopathogenic fungi, the influence on soil microbiota, and the development of sugar cane were evaluated. The strains did not show biocontrol activity. In terms of microbiological parameters, the study revealed a significant increase in microbial biomass and soil biological activity, confirming the importance of fertility and nutrient availability. Positively impacting plant development. Thus, bio-inputs based on Azospirillum brasilense and Pseudomonas fluorescens, isolated or by combined fermentation, improved soil quality, soil biological activity, and the availability of plant nutrients.

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