Tissue preservation and cell culture of Przewalskii´s horse (Equus przewalskii): an endangered species


  • Rodrigo Puentes Universidad de la Republica
  • Agustín Furtado Universidad de la Republica
  • Maria José Estradé Universidad de la Republica
  • Rafael Aragunde Universidad de la Republica
  • Nicolás Cazales Universidad de la Republica
  • Gabriela Costa Universidad de la Republica
  • Jorge Estévez Universidad de la Republica
  • Fernando Cirilo Zoológico Municipal Villa Dolores
  • Cecilia Galosi Universidad Nacional de La Plata


The Przewalskii´s horse or Mongolian wild horse (Equus przewalskii, Poljakov, 1881) is presently the only species of wild horse in existence. Originally from Asia, it is, classified as in extremely high risk of extinction which puts the species in the seriously threatened category. The aim of this work was the preservation of tissues and the development of cell cultures from tissue samples obtained from a Przewalskii´s horse after its death. Biopsies of skin, skeletal and cardiac muscle, and ear cartilage were removed from a recently dead horse, added to Phosphate Buffer Saline (PBS) and refrigerated until processing. Some of the samples were frozen in liquid nitrogen and the other was grown as explants to generate fibroblast cell monolayers. The cell cultures obtained, were subsequently propagated with low passages, and frozen in liquid nitrogen, thus avoiding genetic and phenotypic alterations. The tissues and cell cultures were thawed to ascertain their viability by checking its progressive grow in a flask. It was not possible to obtain cultures from cardiac muscle. A bank of tissues and cells from the single Przewalskii´s horse that existed in Uruguay was generated, and can be used for scientific purposes and for the conservation of the species in the future.


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How to Cite

PUENTES, R., FURTADO, A., ESTRADÉ, M.J., ARAGUNDE, R., CAZALES, N., COSTA, G., ESTÉVEZ, J., CIRILO, F. and GALOSI, C., 2011. Tissue preservation and cell culture of Przewalskii´s horse (Equus przewalskii): an endangered species . Bioscience Journal [online], vol. 27, no. 5, pp. 826–829. [Accessed24 February 2024]. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/8064.



Agricultural Sciences