Effects of brewer’s spent yeast concentrations and application methods on Coffea arabica seedling development
DOI:
https://doi.org/10.14393/BJ-v42n0a2026-72512Keywords:
Agro-industrial by products, Coffea arabica L, Plant growth promotion, Sacharomyces cerevisiae.Abstract
Brewer’s spent yeast (BSY) contains Saccharomyces cerevisiae, which is a byproduct that may be used as a biofertilizer and plant growth promoter. Improving the understanding of the ideal BSY concentration and application method for coffee seedling development will enable more targeted application strategies. We hypothesized that a given BSY concentration and application method would improve the development of coffee seedlings by adding sources of organic matter containing yeast. This hypothesis was tested by assessing the development parameters of Coffea arabica var. Typica (Cramer variety) using different concentrations (0, 2.35, 4.71, 9.40, 11.75 x 108 S. cerevisiae cells mL-1) and two application methods (foliar-FA and soil-SA). Our analysis revealed that BSY foliar application was more efficient than soil application in improving coffee seedling development. Foliar application at 2.3 x 108 S. cerevisiae cells mL-1 increased fresh shoot weight (2.35 times), fresh root weight (4.22 times), dry shoot weight (2.23 times), and dry root weight (3.62 times) in coffee plants compared to the negative control (sterile solution). Our findings provide evidence of waste reuse as an alternative method for developing coffee seedlings through the addition of materials rich in organic matter and yeast. They also align with the Sustainable Development Goals (SDGs), demonstrating strategies that support sustainable agriculture and circular economy practices.
References
AL-HUQAIL, Arwa Abdulkreem. Stimulating the efficiency of Cd-phytoremediation from contaminated soils by Solanum nigrum L.: Effect of foliar and soil application of yeast extract. South African Journal of Botany. 2023 161, 512-518. https://doi.org/10.1016/j.sajb.2023.08.053
ARAÚJO, V. C., et al. Enhanced growth in nursery of coffee seedlings inoculated with the rhizosphere fungus Aspergillus niger for field transplantation. Rhizosphere. 2020, 15, 100236. https://doi.org/10.1016/j.rhisph.2020.100236
BOTHA, A. The importance and ecology of yeasts in soil. Soil Biology and Biochemistry. 2011, 43, 1-8. https://doi.org/10.1016/j.soilbio.2010.10.001
CABRINI, P. G., et al. Torulaspora globosa: rhizosphere yeast promoting lettuce growth on seedlings and under field conditions. Horticultura brasileira. 2019, 37, 266-271. http://dx.doi.org/10.1590/S0102-053620190303
CHETRARIU, A., et al. Spent Grain: A Functional Ingredient for Food Applications. Foods. 2023, 12(7), 1533. https://doi.org/10.3390/foods12071533
CIOCAN, Marius Eduard et al. Use of Unmalted and Malted Buckwheat in Brewing. Applied Sciences. 2023, 13(4), 2199. https://doi.org/10.3390/app13042199
DAS, P. P. et al. Plant-soil-microbes: A tripartite interaction for nutrient acquisition and better plant growth for sustainable agricultural practices. Environmental Research. 2022, 214, 113821. https://doi.org/10.1016/j.envres.2022.113821
DJUKIĆ-VUKOVIĆ, A. et al. Wastes from bioethanol and beer productions as substrates for l (+) lactic acid production–A comparative study. Waste Management. 2016, 48, 478-482. https://doi.org/10.1016/j.wasman.2015.11.031
DUONG, B. et al. Coffee microbiota and its potential use in sustainable crop management. A review. Frontiers in Sustainable Food Systems. 2020, 4, 607935. https://doi.org/10.3389/fsufs.2020.607935
FAN, M. et al. Characteristics analysis of solid waste generation and carbon emission of beer production in China. Environmental Research. 2024, 245, 118017. https://doi.org/10.1016/j.envres.2023.118017
FERNANDEZ-SAN MILLAN, A. et al. Plant growth-promoting traits of yeasts isolated from Spanish vineyards: Benefits for seedling development. Microbiological research. 2020, 237, 126480. https://doi.org/10.1016/j.micres.2020.126480
FONSECA, J.V. et al. Influência de doses e fonte de fertilizantes na produtividade do cafeeiro. Cerrado Agrociências. 2022, 13, 66-74.
FREIMOSER, F. M. et al. Biocontrol yeasts: Mechanisms and applications. World Journal of Microbiology and Biotechnology. 2019, 35, 1-19. https://doi.org/10.1007/s11274-019-2728-4
GIANNAKOU, K. et al. Biotechnological exploitation of Saccharomyces jurei and its hybrids in craft beer fermentation uncovers new aroma combinations. Food Microbiology. 2021, 100, 103838. https://doi.org/10.1016/j.fm.2021.103838
HERRERA-BALANDRANO, D. D. et al. Antagonistic mechanisms of yeasts Meyerozyma guilliermondii and M. caribbica for the control of plant pathogens: A review. Biological control. 2023, 186, 105333. https://doi.org/10.1016/j.biocontrol.2023.105333
HESHAM, A. E. et al. Molecular genetic identification of yeast strains isolated from Egyptian soils for solubilization of inorganic phosphates and growth promotion of corn plants. Journal of microbiology and biotechnology. 2011, 21(1), 55-61. https://doi.org/10.4014/jmb.1006.06045
JAEGER, A. et al. Brewer’s spent yeast (BSY), an underutilized brewing by-product. Fermentation. 2020, 6(4), 123. https://doi.org/10.3390/fermentation6040123
JOHNSON, L. F. et al. Methods for research on the ecology of soil-borne plant pathogens. Methods for research on the Ecology of Soil-Borne Plant Pathogens. 1972.
KAPOOR, D. and KARNWAL, A. Yeast as plant growth promoter and biocontrol agent. In: SHARMA, V.K.; SHAH, M.P.; PARMAR, S.; KUMAR A. (eds.), Fungi bio-prospects in sustainable agriculture, environment and nano-technology. Academic Press. 2021, 429–457.
LU, L. et al. Comprehensive review of fungi on coffee. Pathogens. 2022, 11 (4), 411. https://doi.org/10.3390/pathogens11040411
MATA, R.; RATINHO, S.; FANGUEIRO, D. Assessment of the environmental impact of yeast waste application to soil: an integrated approach. Waste and Biomass Valorization. 2019, 10, 1767-1777. https://doi.org/10.1007/s12649-017-0168-7
MARTINEZ, F. et al. A machine learning model for the diagnosis of coffee diseases. International Journal of Advanced Computer Science and Applications. 2022, 13(4). https://doi.org/10.14569/ijacsa.2022.01304110
MCCARTHY A. L. et al. Brewers’ spent grain; bioactivity of phenolic component, its role in animal nutrition and potential for incorporation in functional foods: a review. Proceedings of the Nutrition Society. 2013, 72 (1),117–125. https://doi.org/10.1017/S0029665112002820
MEDEIROS, E.V. et al. Biochar and Trichoderma as an eco-friendly and low-cost alternative to improve soil chemical and biological properties. Waste Biomass Valor. 2023, 1–12. https://doi.org/10.1007/s12649-023-02240-2
MUKHERJEE, A. et al. Yeast a potential bio-agent: future for plant growth and postharvest disease management for sustainable agriculture. Appl Microbiol Biotecnologia. 2020, 104 (4), 1497–1510. https://doi.org/10.1007/s00253-019-10321-3
MUSSATTO et al. Brewers' spent grain: generation, characteristics and potential applications. Journal of Cereal Science. 2006, 43 (1), 1–14. https://doi.org/10.1016/j.jcs.2005.06.001
NEVES, O.S.C. et al. Growth, mineral nutrition and foliar critical level of K in umbuzeiro seedlings, in function of the potassium fertilization. Ciência e Agrotecnologia. 2007, 31 (3), 636–642. https://doi.org/10.1590/S1413-70542007000300006
PIVA, R.C. et al. Manufacturing and characterization of craft beers with leaves from Ocimum selloi Benth. Journal of Food Science and Technology. 2021, 58 (11), 4403–4410. https://doi.org/10.1007/s13197-020-04925-1
PRADO, R.D.M. et al. Nitrogen, phosphorus and potassium on nutrition and production of seedlings of ‘valencia’ orange. Revista Brasileira de Fruticultura. 2008, 30(3), 812–817. https://doi.org/10.1590/S0100-29452008000300042
R CORE TEAM. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. 2023.
RAMOS-GARZA, J. et al. Mycobiota of Mexican Maize Landraces with Auxin-Producing Yeasts That Improve Plant Growth and Root Development. Plants. 2023, 12(6), 1328. https://doi.org/10.3390/plants12061328
SILVA, J. S. A. et al. Biochar and Trichoderma aureoviride URM 5158 as alternatives for the management of cassava root rot. Applied Soil Ecology. 2022, 172, 104353. https://doi.org/10.1016/j.apsoil.2021.104353
SIPICZKI, M. Identification of antagonistic yeasts as potential biocontrol agents: Diverse criteria and strategies. International Journal of Food Microbiology. 2023, 406, 110360. https://doi.org/10.1016/j.ijfoodmicro.2023.110360
SOUZA, C. et al. Biotechnological Applications of Nonconventional Yeasts. Yeasts in Biotechnology. 2019, 1–27. https://doi.org/10. 5772/57353
TENÓRIO, D. A. et al. Biological control of Rhizoctonia solani in cowpea plants using yeast. Tropical Plant Pathology. 2019, 44, 113–119. https://doi.org/10.1007/s40858-019-00275-2
VENTURA, J. A. et al. Impact and management of diseases in the propagation of fruit plants. Revista Brasileira de Fruticultura, 2017, 41(4), e-647. https://doi.org/10.1590/0100-29452019647
WANG, B. et al. Improvement of protein bait produced from beer yeast waste for controlling Bactrocera dorsalis (Diptera: Tephritidae) in China. Journal of Asia-Pacific Entomology. 2021, 24(3), 573–579. https://doi.org/10.1016/j.aspen.2021.04.017
ZHANG, S. J. et al. Influence of various processing parameters on the microbial community dynamics, metabolomic profiles, and cup quality during wet coffee processing. Frontiers in Microbiology. 2019, 10, 2621. https://doi.org/10.3389/fmicb.2019.02621
Downloads
Published
Issue
Section
License
Copyright (c) 2026 Everlaine Leopoldino Dias Silva, Jamilly Alves de Barros, Diogo Paes da Costa , Carlos Fragoso de Souza, Rachel Lyra Neves, Wallace Rodrigues Telino Júnior, Erika Valente de Medeiros
This work is licensed under a Creative Commons Attribution 4.0 International License.
