Genotypic reaction of chickpea to Fusarium oxysporum and seed sanitary quality

Authors

DOI:

https://doi.org/10.14393/BJ-v40n0a2024-71926

Keywords:

Cicer arietinum, Fungicide, Fusarium, Pathogenicity, Seeds.

Abstract

This research investigated the reaction of chickpea genotypes to Fusarium oxysporum CML 2878, seed health quality, and seed treatment with Carbendazim + Thiram fungicide. The roots of 15-day-old chickpea seedlings were injured, immersed in fungal suspension (4.5 x 107 conidia/mL), and transplanted. Thirty days later, F. oxysporum incidence in the genotypes, pathogenicity, and fresh mass and length of the root and shoot were evaluated. The conducted seed health test followed the incubation methodology on a paper substrate. The seed health and sand emergence tests used a factorial arrangement with two seed treatments (treated with fungicide and untreated) and nine genotypes. Total emergence and the number of normal and abnormal plants were evaluated. All genotypes were susceptible to F. oxysporum CML 2878, thus yellowing and browning the leaves and reducing root development. The primary fungi associated with the seeds of the studied genotypes were Aspergillus sp., Fusarium sp., Rhizopus sp., and Penicillium sp. The fungicide was highly efficient in fungal control but reduced emergence, weight, seedling height, and the number of normal seedlings in the genotypes. This study demonstrated that the evaluated genotypes were susceptible to F. oxysporum CML 2878 and Fusarium sp., spread by seeds. Although fungicidal control was efficient for Fusarium, it may interfere with chickpea germination and vigor.

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References

ALI, M.E., et al. Sensitivity of Aspergillus flavus isolates from peanut seeds in Georgia to Azoxystrobin, a Quinone outside Inhibitor (QoI) Fungicide. Journal of Fungi. 2021, 7(4), 284. https://doi.org/10.3390/jof7040284

AZEVEDO, D.M.Q., et al. Antagonistic effect of Trichoderma isolates and its metabolites against Fusarium solani and F. oxysporum in chickpea. Brazilian Journal of Development. 2020, 6(6), 36344–36361. https://doi.org/10.34117/bjdv6n6-251

AZEVEDO, D.M.Q., et al. Etiology of root rot and wilt disease of chickpea in Brazil. Tropical Plant Pathology. 2017, 42(4),273–283. https://link.springer.com/article/10.1007/s40858-017-0145-5

BARNETT, H. L. and HUNTER, B. B. 1998. Illustrated genera of imperfect fungi. 4th Edition, APS Press, St. Paul, 218p. Available from: https://www.academia.edu/35499449/Illustrated_genera_of_imperfect_fungi_fourth_edition_Barnett_y_Hunter_pdf_pdf

BEKELE, D. et al. The extent and association of chickpea Fusarium wilt and root rot disease pressure with major biophysical factors in Ethiopia. Journal of Plant Pathology. 2021, 103, 409-419. https://link.springer.com/article/10.1007/s42161-021-00779-4

BHAR, A., JAIN, A., and DAS, S. Soil pathogen, Fusarium oxysporum induced wilt disease in chickpea: a review on its dynamicity and possible control strategies. Proceedings of the Indian National Science Academy. 2021, 87(2), 260-274. https://link.springer.com/article/10.1007/s43538-021-00030-9

BRASIL. Ministério da Agricultura e Reforma Agrária. Regras para análises de sementes. Brasília: SNDA/DNDV/CLAV, 2009a. 398p. http://https://www.gov.br/agricultura/ptbr/assuntos/insumos-agropecuarios/arquivos-publicacoes-insumos/2946_regras_analise__sementes.pdf

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Manual de análise sanitária de sementes. Brasília-DF, 2009b. 202p. Available from: https://www.gov.br/agricultura/pt-br/assuntos/insumos-agropecuarios/insumos-agricolas/sementes-e-mudas/publicacoes-sementes-e-mudas/manual-de-analise-sanitaria-de-sementes/view

CARVALHO, E.R. et al. Phytotoxicity in soybean seeds treated with phytosanitary products at different application times. Journal of Seed Science. 2020, 42, 1-12. https://doi.org/10.1590/2317-1545v42237847

COTA-BARRERAS, C.I., et al. Phylogeny, distribution, and pathogenicity of fusarioid fungi associated with chickpea wilt in Sinaloa and Sonora, Mexico. Research Square. 2023, 1, 1-20. https://doi.org/10.21203/rs.3.rs-2960826/v1

ERASTO, R., KILASI, N. and MADEGE, R.R. Prevalence and management of phytopathogenic seed-borne fungi of maize. Seeds. 2023, 2, 30-42. https://www.mdpi.com/2674-1024/2/1/3

FAOSTAT. Food and Agricultural Organization of the United Nation, FAO Statistical Database. 2022. Available from: https://www.fao.org/faostat/en

GARCIA, P.C., et al. Is the application of carbendazim harmful to healthy plants? Evidence of weak phytotoxicity in tobacco. Journal Agricultural and Food Chemistry. 2002, 50(2), 279-283. https://doi.org/10.1021/jf010748g

GETANEH, G., et al. Seed quality and mycoflora associated with chickpea (Cicer arientinum L.) seed in Ethiopia. African Journal of Food, Agriculture Nutrition and development. 2020, 20(6), 16761-16778. https://doi.org/10.18697/ajfand.94.18315

GILBERT, G.S., DIAZ, A. and BREGOFF, H.A. Seed disinfestation pratices to control seed-borne fungi and bacteria in home production of sprouts. Foods. 2023, 12(4), 2-17. https://doi.org/10.3390/foods12040747

GOLAKIYA, B.B., BHIMANI, M.D. and AKBARI, L.F. Efficacy of different fungicides for the management of chickpea wilt (Fusarium oxysporum f. sp. ciceri). International Journal of Chemical Studies. 2018, 6(2), 199-205. https://www.chemijournal.com/archives/2018/vol6issue2/PartC/6-1-341-525.pdf

HAZZAT, N.E., et al. Fusarium equiseti as one of the main Fusarium species causing wilt and root rot of chickpeas in Morocco. Acta Mycologica. 2023, 57, 1-10. https://www.journalssystem.com/amy/-Fusarium-equiseti-as-One-of-the-Main-Fusarium-Species-Causing-Wilt-and-Root-Rot,171904,0,2.html

KHATUN, A., SHAMSI, S. and BASHAR, M.A. Diversity of seed borne fungi associated with fourteen varieties of storage cotton (Gossypium hirsutum L.) seeds. Journal of Biodiversity Conservation and Bioresource Management. 2018, 4(2), 43–52. https://doi.org/10.3329/jbcbm.v4i2.39846

LESLIE, J. F. and SUMMERELL B. A. The Fusarium laboratory manual. Blackwell Publishing, Hoboken, 1-2. 2006. 388 p. https://doi.org/10.1002/9780470278376

MOPARTHI, S., et al. Fusarium spp. Associated with root rot of pulse crops and their cross-pathogenicity cereal crops in Montana. Plant Disease. 2021, 105, 548-557. https://doi.org/10.1094/PDIS-04-20-0800-RE

NAHAR, M.N., HOSEN, S. and SHAMSI, S. Prevalence of fungi associated with seeds of three cotton varieties (Gossypium arboreum L.) in storange. Bioresearch Communications. 2019, 5(1),642-648. https://www.bioresearchcommunications.com/index.php/brc/article/view/73/79

NASCIMENTO, W.M. and SILVA, P.P. Grão-de-bico nova aposta do agronegócio brasileiro. Ed. 23, 2019. https://seednews.com.br/artigos/2969-grao-de-bico-nova-aposta-do-agronegocio-brasileiro-edicao-maio-2019

PANDE, S., RAO, J.N. and SHARMA, M. Establisment of the chickpea wilt pathogen Fusarium oxysporum f. sp. ciceris in the soil through seed transmission. The plant Pathology Journal. 2007, 23(1), 3-6.

QURESHI, M.A., ARSIA, SK. and KUMAR, A. Variability among the isolates of Fusarium oxysporum f.sp. ciceri in Nimar region of Madhya Pradesh. The Pharma Innovation Journal. 2021, 10(4), 46-50. https://www.thepharmajournal.com/archives/2021/vol10issue4/PartA/10-9-276-618.pdf

REHMAN, S.U., et al. Comparative efficacy of Trichoderma viride and Trichoderma harzianum against Fusarium oxysporum f sp. ciceris causing wilt of chickpea. African Journal of Microbiology Research. 2013, 7(50), 5731-5736. http://www.academicjournals.org/AJMR

ROCHA, F.S., et al. Damage caused by different inoculum levels of Aspergillus ochraceus on the vigour of soybean seeds. Semina: Ciências Agrárias. 2014, 35(6), 2895-2904. https://doi.org/10.5433/1679-0359.2014v35n6p2895

ROCHA, F.S., MELO, M.P. and MUNIZ, M.F.S. Fusarium wilt and rot root in chickpea: taxonomy, symptomatology and etiology. Brazilian Journal of Development. 2020, 6(7), 48919-48932. https://ojs.brazilianjournals.com.br/ojs/index.php/BRJD/article/view/13584

RODRIGUES, A.A.C. and MENEZES, M. Identification and pathogenic characterization of endophytic Fusarium species from cowpea seeds. Mycopathologia. 2005, 159, 79-85. Available from: https://pubmed.ncbi.nlm.nih.gov/15750735/

SAIDO, K.A., MOHAMMED, H.I. and HALEEM, R.A. Isolation and identification of fungi associated with different species of stored graines. Journal of Univesity of Duhok. 2020, 23(2), 259-266. https://doi.org/10.26682/ajuod.2020.23.2.29

YADAV, A.S., YADAV, S. and SINGH, R. Screening of fungicides in vitro and seed treatment against Fusarium wilt of chickpea caused by Fusarium oxysporum f.sp. ciceris. Journal of Pharmacognosy and Phytochemistry. 2018, 7(3), 2908-2911. https://www.phytojournal.com/archives/2018/vol7issue3/PartAM/7-3-314-662.pdf

ZAIM, S., BEKKAR, A.A. and BELABID, L. Efficacy of Bacillus subtilis and Trichoderma harzianum combination on chickpea Fusarium wilt caused by F. oxysporum f. sp. ciceris. Archives of Phytopathology and Plant Protection. 2018, 51(3-4), 217-226. https://doi.org/10.1080/03235408.2018.1447896

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Published

2024-07-17

How to Cite

BARBOSA, D.M.C. do R., ROCHA, F. da S., COSTA, C.A. da, NASCIMENTO, W.M., MIZOBUTSI, E.H. and CATÃO, H.C.R.M., 2024. Genotypic reaction of chickpea to Fusarium oxysporum and seed sanitary quality. Bioscience Journal [online], vol. 40, pp. e40037. [Accessed29 October 2024]. DOI 10.14393/BJ-v40n0a2024-71926. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/71926.

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Section

Agricultural Sciences