Determination of biotechnological potential of poultry litter isolated bacteria

Renata Battisti; Adriana Junkerfuerbom; Kawana Nasser Machado; Dany Mesa; Marise Fonseca dos Santos; Eliane Cristina Gruszka Vendruscolo

doi:10.14393/BJ-v40n0a2024-69345

Authors

Renata Battisti Universidade Federal do Paraná https://orcid.org/0000-0002-3098-3138
Adriana Junkerfuerbom Universidade Federal do Paraná
Kawana Nasser Machado Universidade Federal do Paraná
Dany Mesa Complexo Pequeno Príncipe https://orcid.org/0000-0001-6351-4955
Marise Fonseca dos Santos Universidade Federal do Paraná https://orcid.org/0000-0002-1214-5667
Eliane Cristina Gruszka Vendruscolo Universidade Federal do Paraná https://orcid.org/0000-0002-3287-0006

DOI:

https://doi.org/10.14393/BJ-v40n0a2024-69345

Keywords:

Broiler, Enzymes, Microbiota, Plant Growth Promoting Bacteria.

Abstract

Poultry litter is composed of residues from industrial wood processing, serving as a bed for the animals. It has also been widely used as an agricultural biofertilizer, increasing its economic importance. Few studies report the microbiological composition of this material or its biotechnological potential, which was the objective of this study. The microbiological composition was obtained by 16sRNA genetic characterization, as well as, phosphate solubilization capacity, indole-3-acetic acid (IAA) production, fungal antagonism, and enzymatic capacity. As a result, 67 isolates were obtained, of which 30 were taxonomically positioned and 27 were biochemically characterized. The microbiota genera consisted of Staphylococcus (17%), Brevibacillus (27%), Bacillus (37%), Mammaliicoccus (7%), Isoptericola, Microbacterium, and Oxalicibacterium (3.5% each). None of the isolates presented phosphate solubilization capacity. Regarding IAA production, UFPRCA19 showed the highest IAA content (16.60 µg.mg^-1protein). Eleven isolates were positive for antagonism with Aspergillus spp. and 25 for Trichoderma spp. Activity of proteases, caseinases, lipases, amylases, and cellulases were observed with the relative frequencies of 59%, 29%, 11%, 32%, 18%, and 21%. IAA and enzyme-producing bacteria as well as fungal antagonism were found among the isolates. Among all the isolated genera, Bacillus for synthesizing IAA and commercial enzymes becomes an interesting source for biotechnological purposes.

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References

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