Colletotrichum truncatum transmission via lima bean seeds

Authors

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-66734

Keywords:

Anthracnose, Phaseolus lunatus, Physiological, Water restriction, Seed health.

Abstract

Anthracnose is the primary disease in Phaseolus lunatus cultures, causing severe losses. Inoculation techniques are vital for assessing genotype resistance and control methods at the early stages of seedling development. This study aimed to compare inoculation methods and exposure times of a lima bean seed variety to Colletotrichum truncatum using a completely randomized 4x5 factorial design with five replications. Seed inoculation methods by direct contact with mycelium, mannitol water restrictor, and sucrose water restrictor and immersion in conidia suspension were compared and submitted to substrates containing the developed pathogen or not at exposure times of 0, 36, 60, 84, and 108 hours. Evaluations were made by analyzing the severity, incidence, and disease index for anthracnose and seedling physiological quality under greenhouse conditions. The inoculation method by direct contact with sucrose solute for 36 hours was the most suitable for C. truncatum inoculation in lima bean seeds, providing a higher transmission rate but slightly affecting their physiological parameters. C. truncatum damage to lima bean seed performance increased with longer exposure times, regardless of the inoculation method.

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References

AHN, E., et al. Inoculation and Screening Methods for Major Sorghum Diseases Caused by Fungal Pathogens: Claviceps africana, Colletotrichum sublineola, Sporisorium reilianum, Peronosclerospora sorghi and Macrophomina phaseolina. Plants. 2023, 12(9), 1906. https://doi.org/10.3390/plants12091906

ARAÚJO, D.V., et al. Transmission and effects of Fusarium oxysporum f. sp. vasinfectum on cotton seeds. African Journal of Agricultural. 2016, 11(20), 1815-1823. https://doi.org/10.5897/AJAR2015.10706

BARROCAS, E.M., et al. Desempenho de sementes de algodão submetidas à deficiência hídrica e presença de Colletotrichum gossypii var. cephalosporioides. Bioscience Journal. 2014, 30(2), 421-428.

BASSO, P., BONALDO, S.M. and RUFFATO, S. Avaliação de fungicidas no controle de antracnose e mancha alvo, e no rendimento da cultura da soja. Scientia Agraria Paranaensis. 2015, 14(3), 191-199. https://doi.org/10.18188/sap.v14i3.10275

BEGUM, M.M., et al. Field performance of bio-primed seeds to suppress Colletotrichum truncatum causing damping-off and seedling stand of soybean. Biological Control. 2010, 53(1), 18-23. https://doi.org/10.1016/j.biocontrol.2009.12.001

BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Manual de análise sanitária de sementes/ Ministério da Agricultura, Pecuária e Abastecimento, Secretária de Defesa Agropecuária, Brasília: MAPA/ACS, 2009.

CAMPBELL, C.L. and MADDEN, L.V. Introduction to plant disease epidemiology. New York, United States of America: John Wiley & Sons, 1990. Available from: https://www.cabdirect.org/cabdirect/abstract/19912305030

CARVALHO, E.M.S., BESERRA JUNIOR, J.E.A. and BARGUIL, B.M., 2015. Lima bean diseases. In: FERREIRA, A.S.A., LOPES, A.C.A. and GOMES, R.L.F. eds. Phaseolus lunatus: Diversity, Growth and Production. USA: Nova Science Publishers. pp. 113-133.

CAVALCANTE, G.R., et al. Diversity, Prevalence, and Virulence of Colletotrichum Species Associated with Lima Bean in Brazil. Plant Disease. 2019, 102(3), 680–680. https://doi.org/10.1094/PDIS-11-18-2002-RE

COELHO, D.L.M., et al. Estresse hídrico com diferentes osmóticos em sementes de feijão e expressão diferencial de proteínas durante a germinação. Acta Scientiarum. Agronomy. 2010, 32(3), 491-499. https://doi.org/10.4025/actasciagron.v32i3.4694

CORE TEAM R., A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing, 2018. Available from: https://www.R-project.Org

COSTA, M.L.N., et al. Inoculação de Fusarium oxysporum f. sp. phaseoli em sementes de feijoeiro através de restrição hídrica. Ciência e Agrotecnologia. 2003, 27(5), 1023-1030. https://doi.org/10.1590/S1413-70542003000500008

CUSTÓDIO, C.C., SALOMÃO, G.R. and MACHADO NETO, N.B. Estresse hídrico na germinação e vigor de sementes de feijão submetidas a diferentes soluções osmóticas. Revista Ciência Agronômica. 2009, 40(4), 617-623.

ERKILIC, A., GUVEN, B. and AKGUL, D.S. Effects of some plant activators and plant materials on stem rot disease of peanut and pepper caused by Sclerotium rolfsii. Journal of Turkish Phytopathology. 2006, 35(1-3), 15-28.

GALLI, J.A., PANIZZI, C. and VIEIRA, R.D. Resistência de variedades de soja à morte de plântulas causada por Colletotrichum truncatum. Arquivos do Instituto Biológico. 2007, 74(2), 163-165. https://doi.org/10.1590/1808-1657v74p1632007

GOMES, R.S.S. and NASCIMENTO, L.C. Induction of resistance to Colletotrichum truncatum in lima bean. Arquivos do Instituto Biológico. 2018, 85, 1-7, e0022018. https://doi.org/10.1590/1808-1657000022018

MACHADO, J.C., et al. Uso da técnica de restrição hídrica ou “condicionamento osmótico” em patologia de sementes. Revisão Anual de Patologia de Plantas. 2012, 20, 37-63.

MACHADO, J.C., et al. Inoculação artificial de sementes de soja por fungos, utilizando solução de manitol. Revista Brasileira de Sementes. 2001, 23(2), 95-101.

MACHADO, J.C., et al. Uso da restrição hídrica na inoculação de fungos em sementes de algodoeiro (Gossypium hirsutum). Revista Brasileira de sementes. 2004, 26(1), 62-67. https://doi.org/10.1590/S0101-31222004000100010

MENEZES, V.O., et al. Detecção e influência de Fusarium spp. na qualidade fisiológica de sementes de pepino. Ciência Rural. 2011, 41(2), 193-199. https://doi.org/10.1590/S0103-84782011005000006

MIGLIORINI, P., et al. Métodos de inoculação de Colletotrichum lindemuthianum em sementes de feijão e danos em plântulas. Biotemas. 2017, 30(1), 37-43.

MOTA, J.M.D., et al. Transmission of Colletotrichum truncatum and Macrophomina phaseolina by lima bean seeds. Summa Phytopathologica. 2019, 45(1), 33-37. https://doi.org/10.1590/0100-5405/180868

PEDROSO, D.C., et al. Métodos de inoculação de Alternaria alternata e A. dauci em sementes de salsa e sua influência na qualidade fisiológica. Revista Brasileira de Sementes. 2010, 32(3), 79-85. https://doi.org/10.1590/S0101-31222010000300009

PEREIRA, V.G.C., et al. Exigências agroclimáticas para a cultura do feijão (Phaseolus vulgaris L.). Revista Brasileira de Energias Renováveis. 2014, 3(1), 32-42.

PIVETA, G., et al. Superação de dormência na qualidade de sementes e mudas: influência na produção de Senna multijuga (L. C. Rich.) Irwin & Barneby. Acta Amazônica. 2010, 40(2), 281-288. https://doi.org/10.1590/S0044-59672010000200006

RAOOF, MA. and RAO, T.G.N. A simple screening technique for early detection of resistance to castor wilt. Indian Phytopath. 1996, 49(4), 389-392.

REY, M.S., et al. Inoculação de sementes de feijão comum (Phaseolus vulgaris) com Colletotrichum lindemuthianum usando diferentes níveis de restrição hídrica. Revista Brasileira Agrociência. 2008, 14(4), 112-116.

REY, M.S., et al. Transmissão semente-plântula de Colletotrichum lindemuthinum em feijão (Phaseolus vulgaris). Arquivos do Instituto Biológico. 2009, 76(3), 465-470. https://doi.org/10.1590/1808-1657v76p4652009

RODRIGUES, G.F., et al. Inoculação artificial de Fusarium oxysporum em sementes de Phaseolus vulgaris. Scientia Plena. 2016, 12(7), 1–6. https://doi.org/10.14808/sci.plena.2016.070202

SANTOS, A.F., PARISI, J.J.D. e MENTEM, J.O.M. Patologia de sementes florestais. Colombo: Embrapa Florestas, 2011.

TEIXEIRA, H. e MACHADO, J.C. Transmissibilidade e efeito de Acremonium strictum em sementes de milho. Ciência e Agrotecnologia. 2003, 27(5), 1045-1052. https://doi.org/10.1590/S1413-70542003000500011

YANG, H.C. and HARTMAN, G.L. Anthracnose. In: HARTMAN, G.L., RUPE, J. and SIKORA, E.J. (Eds.) Compendium of Soybean Diseases and Pests. USA: American Phytopathological Society, 2016, pp. 31-34.

YESUF, M. and SANGCHOTE, S. Seed Transmission and epidemics of Colletotrichum lindemuthianum in the major common bean growing areas of Ethiopia. Kasetsart Journal. 2005, 39, 34-45.

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Published

2023-10-09

How to Cite

GOMES, R. dos S.S., SILVA, H.F. da, MEDEIROS, J.G.F., PEREIRA, W.E. and NASCIMENTO, L.C. do, 2023. Colletotrichum truncatum transmission via lima bean seeds. Bioscience Journal [online], vol. 39, pp. e39099. [Accessed19 June 2024]. DOI 10.14393/BJ-v39n0a2023-66734. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/66734.

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Section

Agricultural Sciences