Antifungal activity of Punica granatum linn extracts against Malassezia pachydermatis

Authors

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-66031

Keywords:

External otitis, Medicinal plants, Pomegranate.

Abstract

Malassezia pachydermatis causes external otitis, often affecting dogs, and control methods for this microorganism have been resistant to synthetic antifungals. Therefore, this study evaluated the antifungal activity of aqueous extracts of Punica granatum Linn (AEP) fruit peel dehydrated (AEPd) and in natura (AEPn) against Malassezia pachydermatis. The M. pachydermatis samples were from the Microbiology Laboratory of the State University of Northern Paraná (UENP), PR, Brazil. The strains were identified and replicated after inoculation in the Sabouraud dextrose medium. Subsequently, the P. granatum extract was obtained through different extraction methods: cold, water bath, decoction, and infusion. Each test was run fivefold at 10, 20, 30, 40, and 50% after 10, 20, and 30 minutes. The sensitivity of isolates was determined with the Kirby-Bauer disc diffusion method and indicated by an inhibition zone larger than 15 mm. The results were evaluated with a 2x3x6 factorial study design, ANOVA, and Tukey's test at 5% significance. AEPn showed antifungal activity on M. pachydermatis strains, and AEPd did not present an inhibitory influence at any concentration and time.  Extraction by decoction was the most efficient, followed by water bath, cold, and infusion. The extracts at a 50% concentration showed the best results, but all other doses determined an inhibition zone larger than 15 mm. Thus, AEP showed a significant therapeutic potential for controlling M. pachydermatis.

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References

ABDU, O.H., SAEED, A.A. and FDHEL, T.A. Polyphenols/flavonoids analysis and antimicrobial activity in pomegranate peel extracts. Electronic Journal of University of Aden for Basic and Applied Sciences. 2020, 1(1), 14-19.https://doi.org/10.47372/ejua-ba.2020.1.4

AL-DEFIERY, M.E. Antimicrobial activity of garlic and Pomegranate Peel extracts against some pathogenic bacteria. IOP Conference Series: Earth and Environmental Science. 2021, 722(1), 012017.https://doi.org/10.1088/1755-1315/722/1/012017

BAJWA, J. Canine otitis externa — Treatment and complications. The Canadian Veterinary Journal. 2019, 60(1), 97-99.

BASSIRI-JAHROMI, S., et al. In Vivo Comparative Evaluation of the Pomegranate (Punica granatum) Peel Extract as an Alternative Agent to Nystatin against Oral Candidiasis. Iranian Journal of Medical Sciences. 2018, 43(3), 296-304.

CARDOSO, R.C., et al. Potencial Antimicrobiano do Óleo da Folha de Eucalyptus urograndis Frente Staphylococcus aureus. Revista Multidisciplinar e de Psicologia. 2019, 13(43), 989-1002.http://idonline.emnuvens.com.br/id

CHAN, W.Y., et al. In vitro antimicrobial activity of monensin against common clinical isolates associated with canine otitis externa. Comparative Immunology, Microbiology and Infectious Diseases. 2018, 57, 34-38.

DEEGAN, K.R., et al. Susceptibility of Malassezia pachydermatis Clinical Isolates to Allopathic Antifungals and Brazilian Red, Green, and Brown Propolis Extracts. Frontiers in Veterinary Science. 2019, 6, 460.https://doi.org/10.3389/fvets.2019.00460

EASA, S.M.H. Antifungal Activities of Some Plant Extracts against Pathogenic Fungi. Egyptian Academic Journal of Biological Sciences. 2018, 10(1), 1-19.

FRANZENER, G., et al. Atividade antifúngica e indução de resistência em trigo a Bipolaris sorokiniana por Artemisia camphorata. Acta Scientiarum Agronomy. 2003, 25(2), 503-507.https://doi.org/10.4025/actasciagron.v25i2.2124

GHOSH, S., et al. Comparison of different aqueous extraction methods for optimum extraction of polyphenols and in vitro anti-oxidant activity from pomegranate peel. Journal of Pharmacognosy and Phytochemistry. 2019, 8(3), 42-347. www.phytojournal.com

GUNJAN, J., et al. Antioxidant activity of various parts of Puniga granatum: A Review. Journal of Drug Delivery & Therapeutics. 2012, 2 (6), 138-141.http://jddtonline.info

KHARCHOUFI, S., et al. Antimicrobial and antioxidant features of ‘Gabsiʼ pomegranate peel extracts. Industrial Crops & Products. 2018, 111, 345–352.https://doi.org/10.1016/j.indcrop.2017.10.037

KHEDOUDJA, K., et al. Demonstration of the antifungal activity of the aqueous macerated extract of pomegranate (Punica granatum Linn.) bark against Rhodotorula sp. International Journal of Minor Fruits, Medicinal and Aromatic Plants. 2020, 6 (2), 28- 35.

LEITE, S.J.O., et al. In vitro assessment of the antimicrobial effects of pomegranate (Punica granatum L.) peel decoction on saliva samples. Journal of Basic and Applied Pharmaceutical Sciences. 2014, 35 (1), 25-28.

MADUGULA, P., et al. “Rhetoric to Reality”- Efficacy of Punica granatum Peel Extract on Oral Candidiasis: An In vitro Study. Journal of Clinical and Diagnostic Research. 2017,11(1),114-117. https://doi.org/10.7860/jcdr/2017/22810.9304

MAGALHÃES, N.R., et al. Frequência de Malassezia spp. em cães apresentando otite externa. Scientific Electronic Archives. 2017, 10 (6).http://www.seasinop.com.br/revista/index.php?journal=SEA&page=article&op=view&path%5B%5D=379&path%5B%5D=pdf

NATIONAL COMMITTEE FOR CLINICAL LABORATORY STANDARDS. Método de referência para testes de diluição em caldo para a determinação da sensibilidade a terapia antifúngica das leveduras.2002.https://www.anvisa.gov.br/servicosaude/manuais/clsi/clsi_OPAS1M38-A.pdf.

PARERA, L.A.M. The effect of ethanol extracts of red pomegranate peel (Punica granatum L.) on activities Candida albicans. Ecology, Environment and Conservation. 2019, 25, 81-86.

PEREIRA, J.V., et al. Efeito antibacteriano e antiaderente in vitro do extrato da Punica granatum Linn. sobre microrganismos do biofilme dental. Revista Brasileira de Farmacognosia. 2006, 16 (1), 88-93.

PIRZADEH, M., et al. Pomegranate as a source of bioactive constituents: a review on their characterization, properties and applications. Critical Reviews in Food Science and Nutrition. 2021, 61 (6), 982–999.https://doi.org/10.1080/10408398.2020.1749825

SANTOS, E.P., et al. Punica granatum L. (Romã) e Atividade Antimicrobiana Contra o Biofilme Dental: uma Revisão Bibliográfica. Ensaios e Ciência. 2019, 23 (2), 88-93. https://doi.org/10.17921/1415-6938.2019v23n2p88-93

SAVIKIN, K., et al. Activity guided fractionation of pomegranate extract and its antioxidant, antidiabetic and antineurodegenerative properties. Industrial Crops and Products. 2018, 113, 142–149.https://doi.org/10.1016/j.indcrop.2018.01.031

SHARMA, A.K., et al. Studies on Malassezia Infection in Otitis External of Dogs. Journal of Animal Research. 2017, 7 (1), 197-200. https://doi.org/10.5958/2277-940x.2017.00027.4

SIDRIM, J.J.C. and MOREIRA, J.L.B. Fundamentos clínicos e laboratoriais da micologia médica. Rio de Janeiro: Editora Guanabara Koogan, 1999.

TURRINI, F. Traditional Decoction and PUAE Aqueous Extracts of Pomegranate Peels as Potential Low-Cost Anti-Tyrosinase Ingredients. Applied Sciences. 2020, 10 (8), 2795.https://doi.org/10.3390/app10082795

VENTUROSO, L.R., et al. Influência de diferentes metodologias de esterilização sobre a atividade antifúngica de extratos aquosos de plantas medicinais. Revista Brasileira de Plantas Medicinais. 2010, 12 (4), 499-505.https://doi.org/10.1590/S1516-05722010000400014

VIANNA, U.R., et al. Tópicos especiais em Ciência Animal V. (1ª Edição). Alegre: Universidade Federal do Espírito Santo, 2016. Available from https://cienciasveterinarias.ufes.br/pt-br/topicos-especiais-em-ciencia-animal-teca

WAFA, B.A. Antimicrobial effect of the Tunisian Nana variety Punica granatum L. extracts against Salmonella enterica (serovars Kentucky and Enteritidis) isolated from chicken meat and phenolic composition of its peel extract. International Journal of Food Microbiology. 2017, 241, 123-131.https://doi.org/10.1016/j.ijfoodmicro.2016.10.007

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Published

2023-10-09

How to Cite

CHAMILETE, S.A.M., MATSUMOTO, L.S., DELFINO, F., PIRAJÁ, G.V., SILVA, R.M.G. da and PEIXOTO, E.C.T. de M., 2023. Antifungal activity of Punica granatum linn extracts against Malassezia pachydermatis. Bioscience Journal [online], vol. 39, pp. e39098. [Accessed24 July 2024]. DOI 10.14393/BJ-v39n0a2023-66031. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/66031.

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Section

Agricultural Sciences