Community structure of endophytic fungi in roots and leaves of Fagopyrum mill and Avena sativa in a Chinese northern cold region

Authors

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-65820

Keywords:

Ascomycota, Basidiomycota, Diversity, Illumina high-throughput sequencing, ITS.

Abstract

In order to explore the endophytic fungi of Fagopyrum Mill and Avena sativa, Illumina Miseq high-throughput sequencing was used to analyze the community structure and diversity of endophytic fungi in leaves and roots of buckwheat and oat at the mature stage. The results of community structure showed that there were 205 operational taxonomic units (OTUs) in buckwheat roots and 181 OTUs in buckwheat leaves based on 97% sequence similarity level. There were 152 OTUs and 127 OTUs in the root and the leaf of oat, respectively. At the phylum level, Ascomycota and Basidiomycota were the dominant endophytic fungi in buckwheat roots and leaves, while Ascomycota was the dominant endophytic fungus in oat roots and leaves. Alpha diversity analysis showed that the Ace index, Chao index and Shannon index of buckwheat roots were higher than that of buckwheat leaves, and the three indices of oat roots were also higher than that of oat leaves, indicating that the richness and diversity of endophytic fungi community in roots were higher than that in leaves. Biomarkers were found by significant difference analysis in buckwheat and oat. The endophytic functional groups of buckwheat and oat were mainly distributed in Pathotroph and Saprotroph. The results of this study laid a foundation for fully exploiting the dominant endophytic fungal resources of buckwheat and oat and further developing microbial fertilizers.

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References

BOLGER, A.M., et al. Trimmomatic: A flexible trimmer for Illumina sequence data[J]. Bioinformatics. 2014, 30(15), 2114–2120. https://doi.org/10.1093/bioinformatics/btu170

BONAFACCIA, G., et al. Composition and technological properties of the flour and bran from common and Tartary buckwheat[J]. Food Chemistry. 2003, 80, 9-15. https://doi.org/10.1016/S0308-8146(02)00228-5

BRADY, S.F., et al. Cytoskyrins A and B, newBIA active bisanthraquinones isolated from an endophytic fungus[J]. Organic Letter. 2002. 2(25), 4047-4049. https://doi.org/10.1021/ol006681k

CHEN, O., et al. The role of oat nutrients in the immune system: A narrative review[J]. Nutrients. 2021, 13, 1048. https://doi.org/10.3390/nu13041048

DAI, Y., et al. The differences and overlaps in the seed-resident microbiome of four Leguminous and three Gramineous forages[J]. Microbial Biotechnology. 2020, 13(5), 1461-1476. https://doi.org/10.1111/1751-7915.13618

EDGAR, R.C., et al. UCHIME improves sensitivity and speed of chimera detection[J]. Bioinformatics. 2011, 27:2194–2200. https://doi.org/10.1093/bioinformatics/btr381

EGUCHI, K., et al. Development of a high-performance liquid chromatography method to determine the fagopyrin content of tartary buckwheat (Fagopyrum tartaricum Gaertn.) and common buckwheat (F. esculentum Moench) [J]. Plant Production Science. 2009, 12, 475–480. https://doi.org/10.1626/pps.12.475

HALLMANN, J., et al. Bacterial endophytes in agricultural crops[J]. Canadian Journal of Microbiology. 1997, 43(10), 895–914. https://doi.org/10.1177/0095244305054674

JI. X., et al. A mini-review of isolation, chemical properties and bioactivities of polysaccharides from buckwheat (Fagopyrum Mill) [J]. International Journal Of Biological Macromolecules. 2019, 127, 204-209. https://doi.org/10.1016/j.ijbiomac.2019.01.043

KIM, I.S., et al. Multiple Antioxidative and Bioactive Molecules of Oats (Avena sativa L.) in Human Health[J]. Antioxidants (Basel). 2021, 10(9), 1454. https://doi.org/10.3390/antiox10091454

KLOEPPER, J.W. and BEAUCHAMP, C. J. A review of issues related to measuring colonization of plant roots by bacteria[J]. Canadian Journal of Microbiology. 1992. 38(12), 1219–1232. https://doi.org/10.1139/m92-202

KRKOŠKOVÁ, B., et al. Prophylactic components of buckwheat[J]. Food Research International. 2005, 38, 561–568. https://doi.org/10.1016/j.foodres.2004.11.009

LEE, J.S., et al. Antihyperlipidemic effects of buckwheat leaf and flower in rats fed a high-fat diet [J]. Food Chemistry. 2010, 119, 235-240. https://doi.org/10.1016/j.foodchem.2009.06.014

LEIŠOVÁ-SVOBODOVÁ, L., et al. Diversity and pre-breeding prospects for local adaptation in oat genetic resources[J]. Sustainability. 2019, 11, 6950. https://doi.org/10.3390/su11246950

LIKAR, M., et al. Mycorrhizal status and diversity of fungal endophytes in roots of common buckwheat (Fagopyrum esculentum) and tartary buckwheat (F. tataricum) [J]. Mycorrhiza. 2008, 18(6-7), 309-315. https://doi.org/10.1007/s00572-008-0181-6

LOU, Y., et al. CmpacC regulates mycoparasitism, oxalate degradation and antifungal activity in the mycoparasitic fungus Coniothyrium minitans[J]. Environmental Microbiology. 2016, 17(11), 4711-4747. https://doi.org/10.1111/1462-2920.1301

MAGOC, T. and SALZBERG, S. FLASH: Fast length adjustment of short reads to improve genome assemblies[J]. Bioinformatics. 2011, 27(21), 2957-2963. https://doi.org/10.1093/bioinformatics/btr507

MCINROY, J.A. and KLOEPPER, J. W. Survey of indigenous bacterial endophytes from cotton and sweet corn[J]. Plant & Soil. 1995, 173(2), 337-342. https://doi.org/10.1007/BF00011472

MERT, I.D. The applications of microfluidization in cereals and cereal-based products: An overview[J]. Critical Reviews in Food Science and Nutrition. 2020, 60, 1007–1024. https://doi.org/10.1080/10408398.2018.1555134

NGUYEN, N.H., et al. FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild[J]. Fungal Ecology. 2016, 20, 241-248. https://doi.org/10.1016/j.funeco.2015.06.006

OHNISHI, O. Search for the wild ancestor of buckwheat III. The wild ancestor of cultivated common buckwheat, and of tatary buckwheat[J]. Economic botany. 1998, 52(2), 123-133. https://doi.org/10.1007/BF02861199

PETRINI, O. Fungal endophytes of tree leaves. Andrews J H, Hirano S S eds. Microbial Ecology of Leaves. New York: Spring-Verlag. 1991, 179-197. https://doi.org/10.1007/97814612316849

RASANE, P., et al. Nutritional advantages of oats and opportunities for its processing as value added foods-A review[J]. Journal of Food Science and Technology. 2015. 52, 662–675. https://doi.org/10.1007/s13197-013-1072-1

SEGATA, N., et al. Metagenomic biomarker discovery and explanation[J]. Genome Biology. 2011, 12(6), R60. https://doi.org/10.1186/gb-2011-12-6-r60

SINGH, R., et al. Avena sativa (Oat), a potential neutraceutical and therapeutic agent: an overview[J]. Critical Reviews in Food Science and Nutrition. 2013, 53(2), 126-144. https://doi.org/10.1080/10408398.2010.526725

STEWART, D. and MCDOUGALL, G. Oat agriculture, cultivation and breeding targets: Implications for human nutrition and health[J]. British Journal of Nutrition. 2014, 112, S50–S57. https://doi.org/10.1017/s0007114514002736

SUN, X.P., et al. Uninterrupted expression of Cmsit1 in a sclerotial parasite Coniothyrium minitans leads to reduced growth and enhanced antifungal ability[J]. Frontiers in Microbiology. 2017, 8, 2208. https://doi.org/10.3389/fmicb.2017.02208

SUN, X., et al. Significant host- and environment-dependent differentiation among highly sporadic fungal endophyte communities in cereal crops-related wild grasses[J]. Environmental Microbiology. 2020, 22(8), 3357-3374. https://doi.org/10.1111/1462-2920.15107

TIAN X., et al. Infection of Plasmodiophora brassicae changes the fungal endophyte community of tumourous stem mustard roots as revealed by high-throughput sequencing and culture-dependent methods[J]. PLoS One. 2019. 14(6), e0214975. https://doi.org/10.1371/journal.pone.0214975

WU Q.F., et al. Diversity of endophytic fungal community in leaves of Artemisia argyi based on high-throughput amplicon sequencing[J]. Polish Journal of Microbiology. 2021, 70(2), 273-281. https://doi.org/10.33073/pjm-2021-025

XIAO, X., et al. Two cultivated legume plants reveal the enrichment process of the microbiome in the rhizocompartments[J]. Molecular Ecology. 2017, 26(6), 1641-1651. https://doi.org/10.1111/mec.14027

ZHANG. Q, XU.J.G. Determining the geographical origin of common buckwheat from China by multivariate analysis based on mineral elements, amino acids and vitamins[J]. Scientific Report. 2017, 7(1), 9696. https://doi.org/10.1038/s41598-017-08808-y

ZHAO, J., et al. Enhancement of rutin production in Fagopyrum tataricum hairy root cultures with its endophytic fungal elicitors[J]. Preparative biochemistry & biotechnology. 2014, 44(8), 782-794. https://doi.org/10.1080/10826068.2013.867872

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Published

2023-03-10

How to Cite

GAO, Y., LI, Z. and HAN, Y., 2023. Community structure of endophytic fungi in roots and leaves of Fagopyrum mill and Avena sativa in a Chinese northern cold region. Bioscience Journal [online], vol. 39, pp. e39039. [Accessed13 April 2024]. DOI 10.14393/BJ-v39n0a2023-65820. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/65820.

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Section

Biological Sciences