Artificial pollination and use of growth regulators in atemoya ‘Gefner’ fruits




Annona cherimola x Annona squamosa, Fruit fixation or fruit set, GA3, NAAaa, Parthenocarpy, Plant growth regulators.


The objective was to study the effectiveness of the growth regulator (ANA + GA3) associated or not to the application of adjuvant and artificial pollination in ‘Gefner’ atemoya. The experiment was conducted in the experimental orchard at Florida’s Tropical Research and Education Center. The experimental design was in a randomized block, with 14 treatments, 10 repetitions and 3 flowers per plot.  The highest percentages of fixed fruits were obtained with hand pollination – HP and 450 NAA + 1250 GA3 mg L-1 + adjuvant and HP. The use of hand pollination for ‘Gefner’ atemoya tree proved to be the most efficient method so far. Applying growth regulator without artificial pollination produces parthenocarpic fruits, however with high rate of abortions, and small fruits.  Growth regulators together with hand pollination produces small and uneven fruits, and cause reduction in the fruits’ titratable acidity. The use of adjuvant caused low fixation and toxicity to fruits, and its use is not recommended.


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ALI, A., et al. Temporal and Spatial Characterization of Rainfall Over Central and South Florida. Journal of the American Water Resources Association. 2000, 36(4), 833–848.

AOAC. Official methods of analysis of AOAC International (19o ed). Gaithersburg, Md. USA: INTERNATIONAL, AOAC, 2012.

ARAÚJO, D.C., et al. Flower stages, germination and viability of pollen grains of annona squamosa l. In tropical conditions. Acta Scientiarum Technology, 2021, 43 (1), e51013.

CHAGAS, P.C., et al. Effect of naphthalene acetic acid, gibberellic acid, and spray adjuvant alone or in conjunction with hand pollination on fruit set and quality of ‘LeahReese’ sugar apple (Annona squamosa L.). Scientia Horticulturae, 2022, 299, 111011.

CZARNOTA, M. and THOMAS, P. Using surfactants, wetting agents, and adjuvants in the greenhouse. University of Georgia, Cooperative Extension, College of Agriculture and Environmental Sciences. 2013.

GALIMBA, K.D., et al. Gibberellic acid induced parthenocarpic ‘Honeycrisp’ apples (Malus domestica) exhibit reduced ovary width and lower acidity. Horticulture Research, 2019, 6(1), 1–13.

HINOJOSA, L., MATANGUIHAN, J.B. and MURPHY, K.M. Effect of high temperature on pollen morphology, plant growth and seed yield in quinoa (Chenopodium quinoa Willd.). Journal of Agronomy and Crop Science, 2019, 205(1), 33–45.

JENKINS, D.A., et al. Attraction of Pollinators to Atemoya (Magnoliales: Annonaceae) in Puerto Rico: A Synergistic Approach Using Multiple Nitidulid Lures. Journal of Economic Entomology, 2013, 106(1), 305–310.

KHALATE, S.M., SUPE, V.S. and DOKE, N.D. Effect of cover crops on pollen viability and fruit set in custard apple (Annona squamosa L.). Trends in Biosciences, 2018, 11(6), 839–841.

KISHORE, K., et al. Pollination biology of Annona squamosa L. (Annonaceae): Evidence for pollination syndrome. Scientia Horticulturae, 2012, 144, 212–217.

KUMAR, R., KHURANA, A. and SHARMA, A.K. Role of plant hormones and their interplay in development and ripening of fleshy fruits. Journal of Experimental Botany, 2014, 65(16), 4561–4575.

LAU, J.Y.Y., et al. Stigmatic exudate in the Annonaceae: Pollinator reward, pollen germination medium or extragynoecial compitum? Journal of Integrative Plant Biology, 2017, 59(12), 881–894.

MARTIN, C., et al. Polyploidy in Fruit Tree Crops of the Genus Annona (Annonaceae). Frontiers in Plant Science, 2019, 10(99), 1-14.

MEADE, C.V. and PARNELL, J.A.N. A revised taxonomy for Uvaria (Annonaceae) in continental Asia. Australian Systematic Botany, 2018, 31(4), 311–356.

MOTA FILHO, V.J.G. Uso de fitorreguladores no desenvolvimento de frutos na atemoieira (Annona cherimola x A. squamosa cv. Gefner). Revista Ceres, 2012, 59(5), 636–645.

MOURA, E.A., et al. Determination of the harvest time of sugar apples (Annona squamosa L.) in function of carpel interspace. Acta Scientiarum. Agronomy, 2021, 43, 2–10.

NOBLE, C.V., DREW, R.M. and SLABAUGH, J.D. Soil survey of Dade County Area. Florida, Washington, DC: Natural Resource Conservation Service, United States Department of Agriculture, 1996.

PACINI, E. and DOLFERUS, R. Pollen Developmental Arrest: Maintaining Pollen Fertility in a World With a Changing Climate. Frontiers in Plant Science, 2019, 10.

PEREIRA, M.C.T., et al. Doses de ácido giberélico na frutificação efetiva e qualidade de frutos de atemoieira “Gefner”. Revista Brasileira de Fruticultura, 2014a, 36(SPE1), 184–191.

PEREIRA, M.C.T., et al. Effects of storage length and flowering stage of pollen influence its viability, fruit set and fruit quality in ‘Red’ and ‘Lessard Thai’ sugar apple (Annona squamosa) and ‘Gefner’ atemoya (A. cherimola×A. squamosa). Scientia Horticulturae, 2014b, 178, 55–60.

PRADO VEROTTI, T., et al. Vegetal regulators increase the quality of atemoya fruits and recover the photosynthetic metabolism of stressed plants. Acta Physiologiae Plantarum, 2019, 41(9), 165.

R CORE TEAM. R: A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing, 2020.

REIG, C., et al. Naphthaleneacetic acid impairs ovule fertilization and early embryo development in loquat (Eriobotrya japonica Lindl.). Scientia Horticulturae, 2014, 165, 246–251.

SANTOS, R.C., et al. Atemoya fruit development and cytological aspects of GA 3 -induced growth and parthenocarpy. Protoplasma, 2019, 256(5), 1345–1360. (31065805).

URBANOWICZ, C., VIRGINIA, R.A. and IRWIN, R.E. Pollen limitation and reproduction of three plant species across a temperature gradient in western Greenland. Arctic, Antarctic, and Alpine Research, 2018, 50(1), S100022.




How to Cite

CHAGAS, P.C., CRANE, J., CHAGAS, E.A., VENDRAME, W., COSTA, B.N.S., NETO, A.R.. and MOURA, E.A. de, 2023. Artificial pollination and use of growth regulators in atemoya ‘Gefner’ fruits. Bioscience Journal [online], vol. 39, pp. e39040. [Accessed13 June 2024]. DOI 10.14393/BJ-v39n0a2023-65135. Available from:



Agricultural Sciences