Cloning the full-length CDNA of actin gene and analysing alliinase gene expression in tillering onion

Authors

  • Yang Yang Ganna Normal University https://orcid.org/0000-0001-6551-8087
  • Ye Song Fu Ganna Normal University
  • Qiu Feng Yang Ganna Normal University
  • Chen Ke Li Ganna Normal University

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-63133

Keywords:

Actin, Alliinase, RACE, Tillering onion.

Abstract

Tillering onion is a herbaceous plant belonging to the Liliaceae family. We cloned the cDNAs of the actin gene (AcACT, GenBank: MF919598) of tillering onion using rapid amplification of the cDNA ends. The full-length cDNA of AcACT was 1,357 bp long with an open reading frame of 1,131 bp encoding 376 amino acids. The amino acid sequence of AcACT shared > 96% similarity with the amino acid sequences of other ACTs and was found (by means of phylogenetic tree analysis) to be closely related to those of Ananas comosus and Papaver somniferum. AcACT expressions showed no significant differences (p > 0.01) in two cultivars L-SH and L-SY over three growth periods and under suitable conditions, low temperature, and short-day conditions. In addition, AcACT was used as an internal reference gene to analyse the expression of the alliinase gene (AcALL). AcALL expression trends in the roots, stems and leaves were consistent with those of diallyl disulphide and diallyl trisulphide. Thus, AcACT is highly conserved and can be used as a suitable internal reference gene when analysing gene expression in tillering onion.

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Published

2023-02-03

How to Cite

YANG, Y., FU, Y.S., YANG, Q.F. and LI, C.K., 2023. Cloning the full-length CDNA of actin gene and analysing alliinase gene expression in tillering onion. Bioscience Journal [online], vol. 39, pp. e39017. [Accessed16 April 2024]. DOI 10.14393/BJ-v39n0a2023-63133. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/63133.

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Section

Agricultural Sciences