Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study

Authors

  • Kerollyn Fernandes Bernardes Silva Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0002-2282-7063
  • Camila Botelho Miguel Centro Universitário de Mineiros
  • Rony Rocha de Oliveira Júnior Centro Universitário Atenas
  • Marcelo Costa Araujo Universidade Federal do Triângulo Mineiro
  • Ferdinando Agostinho Universidade Federal do Triângulo Mineiro
  • Carlos Ueira-Vieira Universidade Federal de Uberlândia https://orcid.org/0000-0002-8369-9069
  • Javier Emilio Lazo-Chica Universidade Federal do Triângulo Mineiro https://orcid.org/0000-0003-1950-1895
  • Wellington Rodrigues Universidade Federal do Triângulo Mineiro

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-63071

Keywords:

Acinetobacter baumannii, Environment, Hospital Infection, Polymerase Chain Reaction.

Abstract

Acinetobacter baumannii is widely recognized in clinical environments due to its infectious capacity, antimicrobial adaptability, and lethality. Analyzing the prevalence of this agent in intra- and extra-hospital environments may reveal target indicators for appropriate management interventions. In this observational cross-sectional study, we evaluated the prevalence of A. baumannii within hospitals with intensive care units and in their external surroundings in a macro-health region of Brazil. Samples of Columba livia (pigeon) droppings from the external environment of four hospitals (n = 40), from floor surfaces (n = 20), and door handles (n = 20) of different hospital wards were collected based on random sampling, all of which were evaluated for the presence of A. baumannii using polymerase chain reactions (PCR). The sensitivity and specificity of the technique was verified after the collected samples were contaminated with clinical samples positive for A. baumannii. We detected a significantly higher A. baumannii prevalence (87.50%, CI = 71.29–100.00) in samples collected within the hospital environment compared with those obtained from the external environment (12.50%, CI = 0.00–28. 71) (p = 0.003). In addition, samples collected from floor surfaces contained bacterial densities (181.3 ± 11.58) that exceeded those in environmental (93.32 ± 1.56) and door handle (142.70 ± 17.14) samples by 94% and 78.71%, respectively. The findings of this study will enhance our understanding of the spatial distribution of A. baumannii and additionally, validate the efficiency of PCR for diagnosis of this infectious agent.

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References

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Published

2023-01-27

How to Cite

SILVA, K..F.B., MIGUEL, C.B., OLIVEIRA JÚNIOR, R.R. de, ARAUJO, M.C., AGOSTINHO, F., UEIRA-VIEIRA, C., LAZO-CHICA, J.E. and RODRIGUES, W., 2023. Molecular analysis of the prevalence of Acinetobacter baumannii in hospitals and the surrounding environments: a cross-sectional study. Bioscience Journal [online], vol. 39, pp. e39019. [Accessed23 June 2024]. DOI 10.14393/BJ-v39n0a2023-63071. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/63071.

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Section

Biological Sciences