Changes in body composition of Wistar rats: effects of high-intensity interval training




Body Composition, High-Intensity Interval Training, Physical Activity, Swimming.


The present study aimed to investigate the effectiveness of high-intensity interval training (HIIT) in the body composition of Wistar rats. The HIIT protocol consisted of high-intensity swimming three times a week for four weeks. There were no differences between groups as to the Lee index. However, the weights of the perigonadal (p=0.001) and retroperitoneal (p=0.026) fats were significantly different between the Control Group (CG, n=10) vs. Trained Group (TG, n=10), respectively. There was also a significant increase in the body weight of the animals in TG (16.43%) and CG (7.19%) at the end of the experiment. These findings suggested that HIIT was not sufficient to improve significantly the body composition of rats.


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ÁGUILA, M.B, et al. Lipid metabolism in rats fed diets containing different types of lipids. Arquivos Brasileiros de Cardiologia. 2002, 78(1), 25-31.

ALKAHTANI, S.A. et al. Effect of interval training intensity on fat oxidation, blood lactate and the rate of perceived exertion in obese men. SpringerPlus. 2013, 2, 532.

BERNARDIS, L.L. and PATTERSON, B.D. Correlation between "Lee index" and carcass fat content in weanling and adult female rats with hypothalamic lesions. The Journal of Endocrinology. 1968, 40(4),527-528.

BATACAN, R.B., et al. Light-intensity and high-intensity interval training improve cardiometabolic health in rats. Applied physiology, nutrition, and metabolism. 2016, 41(9), 945–952.

DE ARAUJO, G.G., et al. Short and Long Term Effects of High-Intensity Interval Training on Hormones. Metabolites. Antioxidant System. Glycogen Concentration. and Aerobic Performance Adaptations in Rats. Frontiers in physiology. 2016, 7, 505.

GARBER, C. E., et al. American College of Sports Medicine position stand. Quantity and quality of exercise for developing and maintaining cardiorespiratory, musculoskeletal and neuromotor fitness in apparently healthy adults: guidance for prescribing exercise. Medicine and science in sports and exercise. 2011, 43(7), 1334-1359.

GIBALA, M.J., et al. Physiological adaptations to low-volume, high-intensity interval training in health and disease. The Journal of Physiology. 2012, 590(5), 1077–1084.

GILLEN, J.B., et al. Twelve Weeks of Sprint Interval Training Improves Indices of Cardiometabolic Health Similar to Traditional Endurance Training despite a Five-Fold Lower Exercise Volume and Time Commitment. PloS one. 2016, 11(4), e0154075.

GROUSSARD, C., et al. Tissue-Specific Oxidative Stress Modulation by Exercise: A Comparison between MICT and HIIT in an Obese Rat Model. Oxidative medicine and cellular longevity. 2019, 2019, 1965364.

HETLELID, K.J., et al. Rethinking the role of fat oxidation: substrate utilisation during high-intensity interval training in well-trained and recreationally trained runners. BMJ open sport & exercise medicine, 2015, 1(1), e000047.

KARLSEN, T., et al. High Intensity Interval Training for Maximizing Health Outcomes. Progress in cardiovascular diseases. 2017, 60(1), 67-77.

KEATING, S.E., et al. Continuous exercise but not high intensity interval training improves fat distribution in overweight adults. Journal of obesity. 2014, 2014, 834865.

KESSLER, H.S, SISSON, S.B. and SHORT, KR. The Potential for High-Intensity Interval Training to Reduce Cardiometabolic Disease Risk. Sports medicine (Auckland, NZ). 2012, 42(6), 489–509.

MARTIN-SMITH, R., et al. High Intensity Interval Training (HIIT) Improves Cardiorespiratory Fitness (CRF) in Healthy, Overweight and Obese Adolescents: A Systematic Review and Meta-Analysis of Controlled Studies. International Journal Environmental Research and Public Health. 2020, 17(8), 2955.

MARZETTI, E., et al. Physical activity and exercise as countermeasures to physical frailty and sarcopenia. Aging clinical and experimental research. 2017, 29(1), 35-42.

NERY, C.S., et al. Medidas murinométricas e eficiência alimentar em ratos provenientes de ninhadas reduzidas na lactação e submetidos ou não ao exercício e natação. Revista Brasileira de Medicina do Esporte. 2011, 17(1), 49-55.

NES, B.M., et al. Exercise patterns and peak oxygen uptake in a healthy population: the HUNT study. Medicine and science in sports and exercise. 2012, 44(10), 1881–1889.

PAES, L., et al. Effects of moderate and high intensity isocaloric aerobic training upon microvascular reactivity and myocardial oxidative stress in rats. PloS one. 2020, 15(2), e0218228.

RAMOS-FILHO, et al. High Intensity Interval Training (HIIT) Induces Specific Changes in Respiration and Electron Leakage in the Mitochondria of Different Rat Skeletal Muscles. PloS one. 2015, 10(6), e0131766.

TERADA, S., et al. Effects of high-intensity swimming training on GLUT-4 and glucose transport activity in rat skeletal muscle. Journal of applied physiology. 2001, 90(6), 2019–2024.

VIEIRA-SOUZA, L.M., et al. Short-term HIIT does not promote oxidative stress or muscle damage. Revista Brasileira de Medicina do Esporte. 2021, 27(2), 138-141.

WEWEGE, M.A., et al, A. High-Intensity Interval Training for Patients with Cardiovascular Disease-Is It Safe? A Systematic Review. Journal of the American Heart Association. 2018, 7(21), e009305.




How to Cite

MARQUES VIEIRA-SOUZA, L., J AIDAR, F., LOPES DOS SANTOS, J., KALININE, E., ALINE LIMA RODRIGUES, J., UILIEN DE OLIVEIRA, J., DENIELLE MATOS DOS SANTOS, J., GETIRANA MOTA, M., WALESKA DOS SANTOS and CARLOS MARÇAL, A., 2023. Changes in body composition of Wistar rats: effects of high-intensity interval training. Bioscience Journal [online], vol. 39, pp. e39014. [Accessed22 April 2024]. DOI 10.14393/BJ-v39n0a2023-62708. Available from:



Biological Sciences