Hematologic parameters of captive Bothrops atrox (Squamata: viperidae)

Heloisa Castro Pereira; Letícia Prata Juliano Dimatteu Telles; Líria Queiroz Luz Hirano; Maísa Paschoal Rios; André Luiz Quagliatto Santos

doi:10.14393/BJ-v38n0a2022-62653

Authors

Heloisa Castro Pereira Universidade Federal de Uberlândia
Letícia Prata Juliano Dimatteu Telles Universidade de Brasília https://orcid.org/0000-0001-6130-0283
Líria Queiroz Luz Hirano Universidade Federal de Uberlândia https://orcid.org/0000-0002-9438-7538
Maísa Paschoal Rios Universidade Federal de Uberlândia
André Luiz Quagliatto Santos Universidade Federal de Uberlândia

DOI:

https://doi.org/10.14393/BJ-v38n0a2022-62653

Keywords:

Blood Count, Clinical Pathology, Leukogram, Reptile.

Abstract

The breeding of venomous snakes in captivity for research purposes and mainly as a source of pharmaceutical products highlights the need to determine hematological parameters for monitoring and ensuring a healthy breeding populationThe complete blood count is used to help diagnose alterations such as anemia, inflammatory diseases, parasitemia, hematopoietic disorders, hemostatic and toxicological changes, as well as bacterial and viral inclusions. Thus, the objective of this study was to define reference parameters for complete blood count in Bothrops atrox snakes. Blood samples were collected from 20 specimens of B. atrox from the Pentapharm do Brasil commercial breeding facility for laboratory examination. Mean values and standard deviation were: hematocrit 33.6 ± 5.47%, hemoglobin 10.81 ± 2.07g/dL, total number of erythrocytes 0.59 ± 0.1 x 106/mm³, leukocytes 11387.5 ± 3279.2/mm³ and thrombocytes 28175 ± 6320/mm³. No significant difference was observed between males and females and heterophils were the predominant leukocyte cell type.

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References

ALMOSNY, N.R.P. and SANTOS, L.C. Laboratory in wild animals, 2000. In: M. FOWLER, ed. Biology medicine and surgery of South American wild animals, Ames: Iowa State University, pp.300-320.

ALMOSNY, N.R.P. Patologia Clínica em Vertebrados Exotérmicos, 2014. In: Z.S. CUBAS, J.C.R. SILVA and J.L. CATÃO-DIAS, eds. Tratado de Animais Selvagens: Medicina Veterinária. São Paulo: Roca, pp. 1597-1623.

AYRES, M., et al. Bioestat – Aplicações estatísticas nas áreas das ciências biomédicas. Belém: Ong Mamiraua, 2007.

BERNARDE, P.S. Mudanças na classificação de serpentes peçonhentas brasileiras e suas implicações na literatura médica. Gazeta Médica da Bahia. 2011, 81(1), 60-69. http://www.gmbahia.ufba.br/index.php/gmbahia/article/view/1141/1076

CAMPBELL, T.W. Clinical Pathology, 2014. In: D.R. MADER and S.J. DIVERS, eds. Current Therapy in Reptile Medicine and Surgery, Missouri: Elsevier Saunders, pp.70-92.

CAMPBELL, T.W. Blood sample collection and preparation in reptiles, 2015. In: T.W. CAMPBELL, ed. Exotic Animal Hematology and Cytology, Iowa: Wiley Blackwell, pp.173-180.

COSTA, H.C. and BÉRNILS, R.S. Répteis do Brasil e suas unidades federativas: lista de espécies. Herpetologia Brasileira. 2018, 7(1), 11-57.

GILLETT, A.K., et al. Haematological and biochemical reference intervals for three species of hydrophiine sea snakes (Hydrophis curtus, H. elegans and H. peronii) in Australia. The Veterinay Journal. 2015, 204(3), 275-281. https://doi.org/10.1016/j.tvjl.2015.04.008

GLASER, V., et al. Parâmetros hematológicos e bioquímicos de Bothropoides jararaca e Bothrops jararacussu (Ophidia-Viperidae) mantidas em cativeiro. Archives of Veterinary Science. 2013, 18(3), 68-74. http://dx.doi.org/10.5380/avs.v18i3.30088

GOMEZ, A., et al. Hematological and biochemical reference intervals for Bothrops asper and Crotalus simus (Serpentes: Viperidae), maintained in captivity for venom extraction. Comparative Clinical Pathology. 2016, 25(3), 615-623. https://doi.org/10.1007/s00580-016-2240-2

GREGO, K.F., et al. Referências hematológicas para a jararaca de rabo branco (Bothrops leucurus) recém capturadas da natureza. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2006, 58(6), 1240-1243. https://doi.org/10.1590/S0102-09352006000600040

HARR, K.E., RASKIN, R.E. and HEARD, D.J. Temporal effects of 3 commonly used anticoagulants on hematologic and biochemical variables in blood samples from macaws and Burmese pythons. Veterinary Clinical Pathology. 2005, 34(4), 383-388. https://doi.org/10.1111/j.1939-165X.2005.tb00065.x

HAWKEY, C.M. and DENNET, T.B. A colour atlas of comparative haematology, Ipswich: Wolfe Medical, 1989.

HEATLEY, J.J. and RUSSELL, K.E. Hematology, 2019. In: S.J. DIVERS and S.J. STAHL, eds. Mader’s Reptile and Anphibian Medicine and Surgery, Missouri: Elsevier, pp. 301-318.

JACOB-FERREIRA, A.L., et al. Antithrombotic activity of Batroxase, a metalloprotease from Bothrops atrox venom, in a model of venous thrombosis. International Journal of Biological Macromolecules. 2017, 95(1), 263-267. https://doi.org/10.1016/j.ijbiomac.2016.11.063

KINDLOVITS, L.M., et al. Aspectos citoquímicos e morfológicos de elementos sanguíneos das serpentes dos gêneros Bothrops e Crotalus mantidas em cativeiro no serpentário do Instituto Vital Brasil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2017, 69(3), 667-675. https://doi.org/10.1590/1678-4162-9072

NARDINI, G., LEOPARDI, S. and BIELLI, M. Clinical hematology in reptilian species. Veterinay Clinics: Exotic Animal Practice. 2013, 16(1), 1-30. http://dx.doi.org/10.1016/j.cvex.2012.09.001

QUADRINI, A.E., et al. Haematological reference of snakes: amazon tree boa (Corallus hortulanus, Linnaeus, 1758) and Burmenese Python (Python bivittatus, Kuhl, 1820) in captive. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2018, 70(4), 1172-1178. https://doi.org/10.1590/1678-4162-9865

SAJEVIC, T., LEONARDI, A. and KRIZAJ, I. Haemostatically active proteins in snake venoms. Toxicon. 2011, 57(5), 627-645. https://doi.org/10.1016/j.toxicon.2011.01.006

TROIANO, J.C., et al. Haematological reference intervals of the south american rattlesnake (Crotalus durissus terrificus, Laurenti, 1768) in captivity. Comparative Haematology International. 1997, 1(7), 109-112. https://doi.org/10.1007/BF02652577

TROIANO, J.C., et al. Haematological and blood chemical values from Bothrops ammodytoides (Ophidia–Crotalidae) in captivity. Comparative Haematology International. 1999, 9(1), 31-35. https://doi.org/10.1007/BF02585519

TROIANO, J.C., et al. Hematological values of some Bothrops species (Ophidia - Crotalidae) in captivity. The Journal Venomous Animals and Toxins. 2000, 6(2), 1-8. https://doi.org/10.1590/S0104-79302000000200005

TROIANO, J.C. Doenças dos répteis, São Paulo: MedVet, 2018.

TRUJILLO, E., et al. Valores hematológicos de Bothrops atrox mantenidos en cautiverio en la ciudad de Lima. Salud y tecnologia veterinaria. 2016, 4(2), 44-52. https://doi.org/10.20453/stv.v4i2.3246

WINTROBE, R. Variations in size and hemoglobin concentration of erythrocytes in the blood of various vertebrates. Folia Haematologie. 1933, 51, 32-49.