Behavioral assessment of Tambaqui juveniles (Colossoma macropomum) exposed to different ambient colors and social stress




Animal behavior, Animal welfare, Color patterns in fish.


Animal welfare regards the quality of life and the environment in which animals live or are exposed. Hence the importance of studies assessing the environmental influence on the biology and behavior of fishes from the Colossoma macropomum species, considering their higher potential for fish-farming and as a test organism in scientific research. This study aimed to assess, in a controlled environment, the behavioral changes expressed by juveniles from the Colossoma macropomum species exposed to different ambient colors or social situations. The results did not show an influence from the different ambient colors or social situations on fish distribution in the water column. The color pattern showed dark tones in black- and blue-colored environments, and light tones in white-colored environments or with the presence of a mirror. The time of locomotor activity/frequency did not change in any of the treatments of exposure to environments with colors and a mirror. Moreover, the fishes remained with folded fins and a straight posture, maintaining a pattern of rhythmic operculum beating in a normal frequency range described for the species. This suggests that animal welfare did not change in any of the situations tested and that there was a pattern of adaptive response to the type of environment.


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ALONSO, M.E., GONZÁLEZ-MONTAÑA J.R., and LOMILLOS, J.M. Consumers' Concerns and Perceptions of Farm Animal Welfare. Animals. 2020; 10(3), 385.

BARBOSA, A.C., et al. Avaliação da taxa metabólica do Tambaqui (Colossoma macropomum) e da tilápia-do-nilo (Oreochromis niloticus). Revista Brasileira de Engenharia de Pesca. 2009, 4(2), 46-55.

BERA, A., et al. Effect of tank colour on foraging capacity, growth and survival of milkfish (Chanos chanos) larvae. Aquaculture. 2019, 512, 734347.

BROWMAN, H.I., et al. Welfare of aquatic animals: Where things are, where they are going, and what it means for research, aquaculture, recreational angling, and commercial fishing. ICES Journal of Marine Science. 2019, 76(1), 82-92.

COSTA, D.C., et al. The effect of environmental colour on the growth, metabolism, physiology and skin pigmentation of the carnivorous freshwater catfish Lophiosilurus alexandri. Journal of Fish Biology. 2016, 90(3), 922-935.

DAWKINS, M.S. Animal welfare and efficient farming: is conflict inevitable? Animal Production Science. 2017, 57, 201-208.

DELGADIN, T.H., et al. Morphological colour adaptation during development in fish: involvement of growth hormone receptor. Journal of Experimental Biology. 2020, 223, jeb230375.

FSBI. Fisheries Society of the British Isles. Fish Welfare (Briefing Paper 2). Cambridge: Granta Information Systems, 2002. Available from:

GONÇALVES-DE-FREITAS, E., et al. Social Behavior and Welfare in Nile Tilapia. Fishes. 2019, 4(2), 23.

GOULDING, M. and CARVALHO, M.L. Life history and management of the Tambaqui (Colossoma macropomum, Characidae); an important Amazonian food fish. Revista Brasileira de Zoologia. 1982, 1(2), 107-133.

HILSDORF, A.W.S. and HALLERMAN, E.M., 2017. Genetic Resources of Freshwater Neotropical Fishes. In: Genetic Resources of Neotropical Fishes. Springer, Cham. 1, 119-210.

HVAS, M., FOLKEDAL, O., and OPPEDAL, F. Fish welfare in offshore salmon aquaculture. Reviews in Aquaculture. 2021, 13(2), 836-852.

KRISTIANSEN, T.S. and BRACKE, M.B.M., 2020. A Brief Look into the Origins of Fish Welfare Science. In: Kristiansen T., Fernö A., Pavlidis M., van de Vis H., eds, The Welfare of Fish. Animal Welfare. Springer, Cham. 20, 1-17.

LINHARES, R.M., PINAGÉ, C.M.M.F., and DUNCAN, W.P. Excessive luminosity fades the skin color of Cardinal tetra. Boletim do Instituto de Pesca. 2018, 44(4), 56-66.

MARANDI, A., et al. Interaction of fish density and background color effects on growth performance, proximate body composition and skin color of common carp, Cyprinus carpio. International Journal of Aquatic Biology. 2018, 6(3), 138-146.

MERIGHE, G.K.F., et al. Efeito da cor do ambiente sobre o estresse social em tilápias do Nilo (Oreochromis niloticus). Revista Brasileira de Zootecnia. 2004, 33(4), 828-837.

MORAIS, I.S. and O'SULLIVAN, F.L.A. Biologia, habitat e cultivo do Tambaqui Colossoma macropomum (CUVIER, 1816). Scientia Amazonia. 2017, 6(1), 81-93.

MCLEAN, E. Fish tank color: An overview. Aquaculture. 2021. 530, 735750.b

NAVARRO, F.K.S.P. and NAVARRO, R.D. Importância das cores no crescimento, bem-estar e reprodução de peixes. Arquivos de Ciências Veterinárias e Zoologia. 2017, 20(1), 45-48.

NINWICHIAN, P., et al. Effects of tank color on the growth, stress responses, and skin color of snakeskin gourami (Trichogaster pectoralis). Aquaculture International. 2018, 26, 659-672.

OLIVEIRA, R.F. and GALHARDO, L. Sobre a aplicação do conceito de bem-estar a peixes teleósteos e implicações para a piscicultura. Revista Brasileira de Zootecnia. 2007, 36, 77-86.

OTSUKA, A., et al. The presence of a co-specific induces risk-taking behaviour and enlargement of somata size of dopaminergic neurons in the brain of male medaka fish. Journal of Fish Biology. 2020, 96(4), 1014-1023.

RUCHIN, A.B. Effect of illumination on fish and amphibian: development, growth, physiological and biochemical processes. Reviews in Aquaculture. 2021, 13(1), 567-600.

SALIS, P., et al. Magic traits in fagic fish: Understanding color pattern evolution using reef fish. Trends in Genetics. 2019, 35(4), 265-278.

SANTOS, E.L., et al. Desempenho de Tambaquis (Colossoma macropomum) submetidos a restrição alimentar e a realimentação em tanques-rede. Arquivo Brasileiro de Medicina Veterinária e Zootecnia. 2018a, 70(3), 931-938.

SANTOS, E.L., et al. Efeito da cor do ambiente sobre o comportamento social de machos de Betta splendens. Nucleus. 2018b, 15(1), 51-60.

SANTOS, T.G., et al. The light intensity in growth, behavior and skin pigmentation of juvenile catfish Lophiosilurus alexandri (Steindachner). Latin American Journal of Aquatic Research. 2019, 47(3), 416-422.

SILVEIRA, M.M., et al. Fighting off the intruder: context-dependent territory defence in the damselfish Stegastes fuscus. Environmental Biology of Fishes. 2020, 103, 1091-1104.

SHAWKEY, M.D. and D'ALBA, L. Interactions between colour-producing mechanisms and their effects on the integumentary colour palette. Philosophical Transactions of the Royal Society B. 2017, 372(1724), 20160536.

SKÖLD, H.N., et al., 2016. Fish Chromatophores-From Molecular Motors to Animal Behavior. In: Kwang W. Jeon, eds, International Review of Cell and Molecular Biology, Academic Press. 321, 171-219.

SLOMAN, K.A., et al. Ethical considerations in fish research. Journal of Fish Biology. 2019, 94(4), 556-577.

SNEDON, L.U., 2020. Can Fish Experience Pain? In: Kristiansen T., Fernö A., Pavlidis M., van de Vis H., eds, The Welfare of Fish. Animal Welfare. Springer, Cham. 20(1), 229-249.

TATEMOTO, P. and SERRA, M. Low Luminosity Reduces Agonistic Behavior of Nile Tilapia. Journal of Applied Animal Welfare Science. 2021, 1-8. [Online ahead of print].

UTNE-PALM, A.C. and SMITH, A., 2020. Fish as Laboratory Animals. In: Kristiansen T., Fernö A., Pavlidis M., van de Vis H., eds, The Welfare of Fish. Animal Welfare. Springer, Cham. 20(1), 375-400.

VISSIO, P.G., et al. Fish skin pigmentation in aquaculture: the influence of rearing conditions and its neuroendocrine regulation. General and Comparative Endocrinology. 2020, 301, 113662.

VOLPATO, G.L., et al. 2020. Behavior and welfare. In: Bernardo Baldisserotto, Elisabeth Criscuolo Urbinati, J.E.P. Cyrino, eds. Biology and Physiology of Freshwater Neotropical Fish, Academic Press, 75-92.

WOOD, C.M., et al. The physiology of the Tambaqui (Colossoma macropomum) at pH 8.0. Journal of Comparative Physiology B. 2017, 188, 393-408.




How to Cite

OSLÉIAS FERREIRA AGUIAR, JOÃO DAVID BATISTA LISBOA, RUY BESSA LOPES, LUCINEWTON SILVA DE MOURA and MAXWELL BARBOSA DE SANTANA, 2023. Behavioral assessment of Tambaqui juveniles (Colossoma macropomum) exposed to different ambient colors and social stress. Bioscience Journal [online], vol. 39, pp. e39012. [Accessed26 March 2023]. DOI 10.14393/BJ-v39n0a2023-62601. Available from:



Biological Sciences