Chemical constituents and in vivo preliminary evaluation of the toxicological activity of Ouratea spectabilis (OCHNACEAE) and Clitoria guianensis (Fabaceae) leaves

Frank Bruno Vieira de Sousa; Állefe Barbosa Cruz; Daniela Francisca Soares; Camila Luiza Cunha; Juliana C. Holzbach

doi:10.14393/BJ-v39n0a2023-62323

Authors

Frank Bruno Vieira de Sousa Universidade Federal do Tocantins https://orcid.org/0000-0003-3215-2351
Állefe Barbosa Cruz Universidade Federal do Tocantins
Daniela Francisca Soares Universidade Federal do Tocantins https://orcid.org/0000-0002-8641-5674
Camila Luiza Cunha Universidade Federal de São Paulo https://orcid.org/0000-0002-7184-0870
Juliana C. Holzbach Universidade Federal do Tocantins https://orcid.org/0000-0003-2489-9359

DOI:

https://doi.org/10.14393/BJ-v39n0a2023-62323

Keywords:

Artemia salina, Cerrado, Kaempferitrin.

Abstract

Clitoria guianensis and Ouratea spectabilis, found in the Brazilian Cerrado, are used in folk medicine, despite the few chemical and biological studies reported in the literature. The present study aims to investigate the toxicity and effect of extracts from both species on the microcrustacean Artemia salina, and to determine the chemical composition of the hexane extract of O. spectabilis leaves and the EtOAc fraction of C. guianensis leaves. Kaempferitrin, a flavonoid isolated from of the EtOAc fraction of C. guianensis leaves, was identified by chemical analysis. Analysis of the hexane extract of O. spectabilis leaves using gas chromatography-mass spectrometry (GC-MS) suggested the presence of twenty-five known substances. The Hex, EtOAc, and EtOH crude extracts of C. guianensis leaves exhibited high and moderate toxicity against Artemia salina, with median lethal dose values (LD₅₀) of 43.7, 25.4, and 233.4 mg.L⁻¹, respectively. The acetone extract of O. spectabilis leaves showed moderate toxicity against Artemia salina with an LD₅₀ value of 115.13 mg.L⁻¹.

Downloads

Download data is not yet available.

References

ARAUJO, M.F., et al. Proposed active compounds from Ouratea parviflora. Journal of Medicinal Plants Research, 2011, 5(12), 2489-2493.

BRANDÃO, G.C., et al. Antiviral activity of plants occurring in the state of Minas Gerais (Brazil): Part III. Journal of Chemical and Pharmaceutical Research. 2011, 3(4), 223-236.

BUSH, R.T. and MCINERNEY, F.A. Leaf wax n-alkane distributions in and across modern plants: implications for paleoecology and chemotaxonomy. Geochimica et Cosmochimica Acta, 2013, 117, 161-179, 2013. https://doi.org/10.1016/j.gca.2013.04.016

CAMPANA, P.R.V., et al. Anti-TNF-α Activity of Brazilian Medicinal Plants and Compounds from Ouratea semiserrata. Phytotherapy Research. 2015, 29(10), 1509-1515. https://doi.org/10.1002/ptr.5401

COE, F.G., PARIKH, D.M. and JOHNSON C. A. Alkaloid presence and brine shrimp (Artemia salina) bioassay of medicinal species of eastern Nicaragua. Pharmaceutical Biology. 2010, 48(4), 439-445. https://doi.org/10.3109/13880200903168015

COLLI, G.R., VIEIRA, C.R. and DIANESE, J.C. Biodiversity and conservation of the Cerrado: recent advances and old challenges. Biodiversity and Conservation. 2020, 29, 1465-1475. https://doi.org/10.1007/s10531-020-01967-x

CUNHA, C.L., et al. New Isoflavone and Other Constituents from Roots of Clitoria guianensis. Journal of the Brazilian Chemical Society. 2020, 31(8), 1753-1757. https://doi.org/10.21577/0103-5053.20200061

ESTEVAM, C.S., et al. Constituintes químicos e avaliação preliminar in vivo da atividade antimalárica de Ouratea nitida Aubl (Ochnaceae). Revista Brasileira de Farmacognosia. 2005, 15(3), 195-198. https://doi.org/10.1590/S0102-695X2005000300005

ESTRABIS, N.V., JUNIOR, J.M. and PISTORI, H. Mapeamento da Vegetação Nativa do Cerrado na Região de Três Lagoas-MS com o Google Earth Engine. Revista Brasileira de Cartografia. 2019, 71(3), 702-725. https://doi.org/10.14393/rbcv71n3-47461

FIDELIS, Q.C., et al. Ouratea genus: chemical and pharmacological aspects. Revista Brasileira de Farmacognosia. 2014, 24(1), 1-19. https://doi.org/10.1590/0102-695X20142413361

GONÇALEZ, E., FELICIO, J.D. and PINTO, M.M. Biflavonoids inhibit the production of aflatoxin by Aspergillus flavus. Brazilian journal of medical and biological research. 2001, 34(11), 1453-1456. https://doi.org/10.1590/S0100-879X2001001100013

HERNÁNDEZ, R.R.R., et al. Biological activities of kaempferitrin-A short review. Pharmacology OnLine. 2017, 3, 79-90.

MCLAUGHLIN, J.L., ROGERS, L.L. and ANDERSON, J. E. The use of biological assays to evaluate botanicals. Drug Information Journal. 1998, 32 513-524. https://doi.org/10.1177%2F009286159803200223

MECINA, G.F., et al. Phytotoxicity of extracts and fractions of Ouratea spectabilis (Mart. ex Engl.) Engl. (Ochnaceae). South African Journal of Botany. 2014, 95, 174-180. https://doi.org/10.1016/j.sajb.2014.10.002

MEYER, B.N., et al. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982, 45, 31-34.

NETO, J.A.R., et al. Using the plants of Brazilian Cerrado for wound healing: From traditional use to scientific approach. Journal of ethnopharmacology. 2020, 260, 112547. https://doi.org/10.1016/j.jep.2020.112547

NGUTA, J.M., et al. Evaluation of Acute Toxicity of Crude Plant Extracts from Kenyan Biodiversity using Brine Shrimp, Artemia salina L. (Artemiidae). The Open Conference Proceedings Journal. 2012, 3, 30-34.

RAHMAN, A.S., et al. Bioactivity guided cytotoxic activity of Clitoria ternatea utilizing brine shrimp lethality bioassay. Bangladesh Journal of Physiology and Pharmacology. 2006, 22(1), 18-21. https://doi.org/10.3329/bjpp.v22i1.3564

ROCHA, M.P., et al. (3,3″) - Linked Biflavanones from Ouratea spectabilis and Their Effects on the Release of Proinflammatory Cytokines in THP 1 Cells. Journal of Natural Products. 2020, 83(6), 1891-1898. https://doi.org/10.1021/acs.jnatprod.0c00074

SANTOS, R.A., DAVID, J.M. and DAVID, J.P. Detection and quantification of rotenoids from Clitoria fairchildiana and its lipids profile. Natural product communications. 2016, 11(5), 631-632.

SIMONI, I.C., et al. Avaliação da citotoxicidade de biflavonoides isolados de Ouratea spectabilis (Ochnaceae) em células de córnea de coelho SIRC. Arquivos do instituto biológico. 2002, 69(4), 95-97.

SOARES, D.F., et al. Phytochemical screening and antioxidant activity of Clitoria guianensis. Revista Cereus. 2020, 12(3), 127-134.

SOUZA, C.D. and FELFILI, J.M. Uso de plantas medicinais na região de Alto Paraíso de Goiás, GO, Brasil. Acta Botanica Brasilica. 2006, 20, 135-142. https://doi.org/10.1590/S0102-33062006000100013

VALENTE, L.M., et al. Kaempferitrin from Uncaria guianensis (Rubiaceae) and its potential as a chemical marker for the species. Journal of the

Brazilian Chemical Society. 2009, 20(6), 1041-1045. https://doi.org/10.1590/S0103-50532009000600007

VAN DEN DOOL, H. and KRATZ, P. A Generalization of the Retention Index System Including Linear Temperature Programmed Gas-Liquid Partition Chromatography. Journal of Chromatography Science. 1963, 11, 463-471. https://doi.org/10.1016/S0021-9673(01)80947-X

VERDE, V.M., PAULA, J.R. and CARNEIRO, D.M. Levantamento etnobotânico das plantas medicinais do cerrado utilizadas pela população de Mossâmedes (GO). Revista Brasileira de Farmacognosia. 2003, 13, 64-66. https://doi.org/10.1590/S0102-695X2003000300024