The role of enzymes in the angiogenic activity of Hancornia speciosa latex
DOI:
https://doi.org/10.14393/BJ-v38n0a2022-61092Keywords:
Chick chorioallantoic membrane, Enzymes, Proteinase KAbstract
The Hancornia speciosa latex has shown angiogenic activity. Angiogenesis plays a major role in wound healing, and materials that stimulate this process could be used to develop drugs. This study aimed to explain the role of proteins in the H. speciosa serum fraction latex in angiogenesis. Hence, this material was treated with proteinase K and the proteins were inactivated. After protein inactivation, angiogenic activity was assessed with the chick chorioallantoic membrane assay. The result showed that the proteins in the serum fraction are responsible for angiogenic activity. Then, the total protein content in the serum fraction and its enzymatic activity were investigated. The low protein content observed in the H. speciosa serum fraction latex suggests that this biomaterial could be used to develop new drugs with a hypoallergenic response. Despite the low protein content, there was a significant enzymatic activity of at least three enzymes in the serum fraction latex: β-1,3 glucanase, β-glucosidase, and proteases. These enzymes seem to influence the healing process, assisting debridement, extracellular matrix remodeling, and collagen deposition, and decreasing the chances of contamination by microorganisms. In conclusion, the enzymes in the H. speciosa serum latex are associated with the angiogenic activity of this biomaterial and may be used to assist the wound healing process.
Downloads
References
ADAIR, T.H. and MONTANI, J.P. Angiogenesis. San Rafael (CA): Morgan & Claypool Life Sciences, 2010. https://doi.org/10.4199/c00017ed1v01y201009isp009
ALEKSANDROWICZ, E. and HERR, I. Ethical euthanasia and short-term anesthesia of chick embryo. Alternative to Animal Experimentation (ALTEX). 2015, 32(2), 143-147. https://doi.org/10.14573/altex.1410031
ALMEIDA, L.M., et al. Hancornia speciosa latex for biomedical applications: physical and chemical properties, biocompatibility assessment and angiogenic activity. Journal of Materials Science: Material in Medicine. 2014, 25(9), 2153-2162. https://doi.org/10.1007/s10856-014-5255-8
ARRUDA, A.S., et al. Mangabeira latex production evaluation in Cerrado region of Goiás. Ciência Florestal. 2016, 26(3), 939-948. https://doi.org/10.5902/1980509824222
BISSWANGER, H. Enzyme Assays. Perspectives in Science. 2014, 1(1-6), 41-55. https://doi.org/10.1016/j.pisc.2014.02.005
BONETE, J.M., et al. Tissue reaction and anti-biofilm action of new biomaterial composed of latex from Hancornia speciosa Gomes and silver nanoparticles. Anais da Academia Brasileira Ciências. 2020, 16(92), e20191584. https://doi.org/10.1590/0001-3765202020191584
BRADFORD M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-Dye Binding. Analytical Biochemistry. 1976, 72(1-2), 248-254. https://doi.org/10.1016/0003-2697(76)90527-3
CAMPOS, A.C.L., BORGES-BRANCO, A. and GROTH, A. Wound healing. Arquivos Brasileiros de Cirurgia Digestiva. 2007, 20(1), 51-58. https://doi.org/10.1590/S0102-67202007000100010
CAVALCANTI, J.M., et al. The essential oil of Croton zehntneri and trans-anethole improves cutaneous wound healing. Journal of Ethnopharmacology. 2012, 144(2), 240-247. https://doi.org/10.1016/j.jep.2012.08.030
CHITHRA, P., SAJITHLAL, G.B. and CHANDRAKASAN, G. Influence of Aloe vera on the glycosaminoglycans in the matrix of healing dermal wounds in rats. Journal of Ethnopharmacology. 1998, 59(3), 179-186. https://doi.org/10.1016/S0378-8741(97)00112-8
COSTA, A.F., et al. Hancornia speciosa serum latex fraction: a non-allergenic biomaterial. Brazilian Journal of Biology. 2023, 83, e251075. https://doi.org/10.1590/1519-6984.251075
COX, M.M., LEHNINGER, A.L. and NELSON, D.L. Lehninger principles of biochemistry. 3th ed. New York: Worth Publishers. 2000. pp. 306–308.
D'ABADIA, P.L., et al. Hancornia speciosa serum fraction latex stimulates the angiogenesis and extracellular matrix remodeling processes. Anais da Academia Brasileira de Ciências. 2020, 92(2), e20190107. https://doi.org/10.1590/0001-3765202020190107
DANCIU, C., et al. Evaluation of phenolic profile, antioxidant, and anticancer potential of two main representants of Zingiberaceae family against B164A5 murine melanoma cells. Biological Research. 2015, 48, 1-9. https://doi.org/10.1186%2F0717-6287-48-1
DEVARAJ, K.B., GOWDA, L.R. and PRAKASH, V. An unusual thermostable aspartic protease from the latex of Ficus racemosa (L.). Phytochemistry. 2008, 69(3), 647–655. https://doi.org/10.1016/j.phytochem.2007.09.003
DE ALMEIDA, L.M., et al. The state-of-art in angiogenic properties of latex from different plant species. Current Angiogenesis. 2015, 4(1), 10-23. http://dx.doi.org/10.2174/221155280401160517164531
DO PRADO, A.D.L., et al. The chick embryo chorioallantoic membrane assay as a model for the study of angiogenesis. Bioscience Journal. 2019, 35(4), 1262–1275. https://doi.org/10.14393/BJ-v35n4a2019-42777
DOS SANTOS NEVES, J., et al. Evaluation of osteogenic potential of Hancornia speciosa latex in rat calvaria and its phytochemical profile. Journal of Ethnopharmacology. 2016, 13(183), 151-158. https://doi.org/10.1016/j.jep.2016.02.041
FLORIANO, J.F., et al. Comparative study of bone tissue accelerated regeneration by latex membranes from Hevea brasiliensis and Hancornia speciosa. Biomedical Physics Engineering Express. 2016, 2(4), e045007. https://doi.org/10.1088/2057-1976/2/4/045007
FOLKMAN J. Fundamental concepts of the angiogenic process. Current Molecular Medicine. 2003, 3(7), 643-651. https://doi.org/10.2174/1566524033479465
HONG, T.Y. and MENG, M. Biochemical characterization, and antifungal activity of an endo-1,3-beta-glucanase of Paenibacillus sp. isolated from garden soil. Applied Microbiology Biotechnology. 2003, 61(5), 472-478. http://dx.doi.org/10.1007/s00253-003-1249-z
JOSE, D., et al. Potential application of beta-1,3 glucanase from an environmental isolate of Pseudomonas aeruginosa MCCB 123 in fungal DNA extraction. Indian Journal of Experimental Biology. 2014, 52(1), 89-96.
JUNQUEIRA, L.C. and CARNEIRO, J. - Tecidos conjuntivos. In JUNQUEIRA, L.C.; CARNEIRO, J. Histologia Básica – Texto e Atlas. 13 ed. Rio de Janeiro. Guanabara Koogan. 2017. p 108-114.
KARIM, A.A., et al. Phenolic composition, antioxidant, anti-wrinkles and tyrosinase inhibitory activities of cocoa pod extract. BMC Complement Alternative Medicine. 2014, 14, 381. http://dx.doi.org/10.1186/1472-6882-14-381
KELLY, K.J. and SUSSMAN, G. Latex allergy: where are we now and how did we get there? The Journal of Allergy Clinical Immunology: In Practice. 2017, 5, 1212-1216. https://doi.org/10.1016/j.jaip.2017.05.029
KIM, Y.J. and HWANG, B.K. Differential accumulation of β-1,3 glucanase and chitinase isoforms in pepper stems infected by compatible and incompatible isolates of Phytophthora capsici. Physiological and Molecular Plant Pathology. 1994, 45(3), 195-209. https://doi.org/10.1016/S0885-5765(05)80077-3
KONNO, K. Plant latex and other exudates as plant defense systems: roles of various defense chemicals and proteins contained therein. Phytochemistry. 2011, 72(13), 1510-1530. https://doi.org/10.1016/j.phytochem.2011.02.016
MACEDO, M. and FERREIRA, A.R. Plantas medicinais usadas no tratamento dermatológico da Bacia do alto Paraguai, Mato Grosso. Revista Brasileira de Farmacognosia. 2004, 14, 40-44. https://doi.org/10.1590/S0102-695X2004000300016
MALMONGE, J.A., et al. Comparative study on technological properties of latex and natural rubber from Harconia speciosa gomes and Hevea brasiliensis. Journal of Applied Polymer Science. 2009, 111(6), 2986–2991. .http://dx.doi.org/10.1002/app.29316
MARINHO, D.G., et al. The latex obtained from Hancornia speciosa Gomes possesses anti-inflammatory activity. Journal of Ethnopharmacology. 2011, 35(2), 530-537. https://doi.org/10.1016/j.jep.2011.03.059
MAURER, H. Bromelain: biochemistry, pharmacology and medical use. Cellular and Molecular Life Sciences CMLS. 2001, 58(9), 1234–1245. https://doi.org/10.1007/pl00000936
MENDONÇA, R.J. and COUTINHO-NETTO, J. Aspectos celulares da cicatrização. Anais Brasileiros de Dermatologia 2009, 84(3), 257-262. http://dx.doi.org/10.1590/S0365-05962009000300007
MONTEIRO, V.N., et al. Trichoderma reesei - Mycoparasitism against Pythium ultimum is coordinated by G-alpha Protein GNA1 Signaling. Journal Microbial Biochemical Technology. 2015, 7(1), 001-007. http://dx.doi.org/10.4172/1948-5948.1000173
NATH, L.K. and DUTTA, S.K. Extraction and purification of curcain, a protease from the latex of Jatropha Curcas Linn. Journal of Pharmacy and Pharmacology. 1991, 43(2), 111–114. https://doi.org/10.1111/j.2042-7158.1991.tb06642.x
NETO, G. and GUARIM-MORAIS, R.G. Medicinal plants resources in the Cerrado of Mato Grosso State, Brazil: a review. Acta Botanica Brasileira. 2003, 17, 561-584. https://doi.org/10.1590/S0102-33062003000400009
NORONHA, E.F. and ULHOA, C.J. Characterization of a 29-KDa ẞ-1,3-glucanase from Trichoderma harzianum. FEMS Lett. 2000, 183(1), 119-123. https://doi.org/10.1111/j.1574-6968.2000.tb08944.x
NOWAK-SLIWINSKA, P., SEGURA, T. and IRUELA-ARISPE, M. The chicken chorioallantoic membrane model in biology, medicine, and bioengineering. Angiogenesis. 2014, 17(4), 779-804. https://doi.org/10.1007/s10456-014-9440-7
PENHAVEL, M.V.C., et al. Efeito do gel da seiva do látex da Hevea brasiliensis na cicatrização de lesões cutâneas agudas induzidas no dorso de ratos. Revista do Colégio Brasileiro de Cirurgia. 2016, 43(1), 48-53. https://doi.org/10.1590/0100-69912016001010
POTT, A. and POTT, V.J. 1994. Plantas do pantanal. EMBRAPA, Planaltina, 320 p.
QUALHATO, T.F., et al. Mycoparasitism studies of Trichoderma species against three phytopathogenic fungi: valuation of antagonism and hydrolytic enzyme production. Biotechnology Letters. 2013, 35, 1461–1468. https://doi.org/10.1007/s10529-013-1225-3
RIBEIRO, T.P., et al. Evaluation of cytotoxicity and genotoxicity of Hancornia speciosa latex in Allium cepa root model. Brazilian Journal of Biology. 2016, 76(1), 245-249. https://doi.org/10.1590/1519-6984.20114
SAMPAIO, T.S. and NOGUEIRA, P.C.L. Volatile componentes of mangaba fruit (Hancornia speciosa Gomes) at three stages of maturity. Food Chemistry. 2006, 95(4), 606-610. http://dx.doi.org/10.1016/j.foodchem.2005.01.038
SARATH, G.; ZEECE, M.G. and PENHEITER, A.R. In Protease assay methods. Proteolytic enzymes: a practical approach, (Beynon, R. and Bond J.S. ed.), Oxford University Press, New York, NY pp. 45-76, 2001.
SHIKSHARTHI, A.R. and MITTAL, S. Ficus racemosa: Phytochemistry, traditional uses and pharmacological properties: a review. International Journal of Recent Advances in Pharmaceutical Research. 2001, 4, 6-15.
SINGER, A.J. and CLARK, R.A. Cutaneous wound healing. The New England Journal of Medicine. 1999, 2(341), 738-746. https://doi.org/10.1056/nejm199909023411006
UDOD, V. and STOROZHUK, V. Treatment of suppurative diseases of soft tissues with proteolytic enzyme, papain. Klinichna khirurhiia. 1979, 1, 37–38.
ULHOA, C.J. and PEBERDY, J.F. Purification and some properties of the extracellular chitinase produced by Trichoderma harzianum. Enzyme and Microbial Technology. 1992, 14(3), 236-240. https://doi.org/10.1016/0141-0229(92)90072-V
URS, A. P., et al. Plant Latex Proteases: Natural Wound Healers. In Proteases in Physiology and Pathology. 2017, 297-323. http://dx.doi.org/10.1007/978-981-10-2513-6_14
USOLTSEVA, R.V., et al. Laminarans and 1, 3-β-D-glucanases. International Journal of Biological Macromolecules. 2020, 15(163), 1010-1025. https://doi.org/10.1016/j.ijbiomac.2020.07.034
WITTENAUER, J., et al. Inhibitory effects of polyphenols from grape pomace extract on collagenase and elastase activity. Fitoterapia. 2015, 101, 179-187. https://doi.org/10.1016/j.fitote.2015.01.005
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Patrícia Lima D'Abadia, Amanda Fernandes Costa, Myllena Tolentino Firmino, Samantha Salomão Caramori, Ruy de Souza Lino-Junior, Fabiana Fonseca Zanoelo, Pablo José Gonçalves, Luciane Almeida
This work is licensed under a Creative Commons Attribution 4.0 International License.