Toxicity of Bacillus sp. (Bacillales: Bacillaceae) on the fungus gnats, Bradysia aff. ocellaris larvae (Diptera: Sciaridae)
DOI:
https://doi.org/10.14393/BJ-v39n0a2023-59878Keywords:
Biological Control, Bti, Strawberry, Sustainable management.Abstract
Bacillus thuringiensis (Bt) Berliner has potential for use in insect management. Its use can be an alternative for the management of Bradysia aff. ocellaris (Comstock), considered one of the main strawberry pests in a soilless system. Therefore, the objective of this work was to evaluate the toxicity of different bacteria on B. aff. ocellaris in laboratory and greenhouse bioassays. The following isolates were used in the experiments: Bacillus circulans (Bc), B. thuringiensis var. oswaldo cruzi (Bto) or B. thuringiensis var. israelensis (Bti) and B. thuringiensis var. kurstaki (Btk) In the laboratory, B. aff. ocellaris larvae showed high susceptibility to Bti isolate (92 % mortality) 14 days after treatment exposure (DAET). In contrast, the isolates Bc, Bto, and Btk showed less than 32 % mortality, not differing from the control treatment (water – 22 % mortality). According to the concentration-response curves the values of lethal concentration LC50 and LC90 were 4 x 106 CFU.mL-1 and 4 x 1015 CFU.mL-1. By applying Bti (4 × 1012 CFU.mL-1) at the base of strawberry plants growing in plastic pots containing commercial plant substrate, a reduction of 26 % in the emergence of B. aff. ocellaris was observed. According to these results, the Bti isolate is considered promising for the formulation of bioinsecticides based on Bt for the management of B. aff. ocellaris in strawberry culture.
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References
ABOUSSAID, H., et al. Biological activity of Bacillus thuringiensis (Berliner) strains on larvae and adults of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). Journal Environmental Protection. 2010, 1, 337–345. https://doi.org/10.4236/jep.2010.14040
ALBEROLA, T.M., et al. Insecticidal activity of strains of Bacillus thuringiensis on larvae and adults of Bactrocera oleae Gmelin (Dipt. Tephritidae). Journal Invertebrate Pathology. 1999, 74, 127–136. https://doi.org/10.1006/jipa.1999.4871
ANDREADIS, S.S., et al. Efficacy of Beauveria bassiana formulations against the fungus gnat Lycoriella ingenua. Biology Control. 2016, 103, 165–171. https://doi.org/10.1016/j.biocontrol.2016.09.003
ARIMOTO, M., et al. Molecular marker to identify the fungus gnat, Bradysia sp. (Diptera: Sciaridae), a new pest of Welsh onion and carrot in Japan. Applied Entomology Zoology. 2018, 53, 419–424. https://doi.org/10.1007/s13355-018-0563-y
BEN-DOV, E. Bacillus thuringiensis subsp. israelensis and its dipteran-specific toxins. Toxins. 2014, 6, 1222–1243. https://doi.org/10.3390/toxins6041222
BRASIL . Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Agrofit—Sistema de agrotóxicos fitossanitários, 2020, Available from: http://agrofit.agricultura.gov.br/agrofit_cons/principal_agrofit_cons
BRAVO, A., GILL, S. S. and SOBERON, M. Mode of action of Bacillus thuringiensis Cry and Cyt toxins and their potential for insect control. Toxicon. 2007, 49, 423–435. http://doi.org./10.1016/j.toxicon.2006.11.022
BROADLEY, A., KAUSCHKE, E. and MOHRIG, W. Black fungus gnats (Diptera: Sciaridae) found in association with cultivated plants and mushrooms in Australia, with notes on cosmopolitan pest species and biosecurity interceptions. Zootaxa. 2018, 4415, 201–242. https://doi.org/10.11646/zootaxa.4415.2.1
BUENTELLO-WONG, S., et al. Characterization of cry proteins in native strains of Bacillus thuringiensis and activity against Anastrepha ludens. Southwest Entomology. 2015, 40, 15–24. https://doi.org/10.3958/059.040.0102
CARVALHO, J.R., et al. Análise de probit aplicada a bioensaios com insetos. Colatina, IFES, 2017.
CASTILHO, R.C., et al. The predatory mite Stratiolaelaps scimitus as a control agent of the fungus gnat Bradysia matogrossensis in commercial production of the mushroom Agaricus bisporus. Integrated Journal Pest Management. 2009, 55, 181–185. https://doi.org/10.1080/09670870902725783
CHEN, C., et al. Detection of insecticide resistance in Bradysia odoriphaga Yang et Zhang (Diptera: Sciaridae) in China. Ecotoxicology. 2017, 26, 868–875. https://doi.org/10.1007/s10646-017-1817-0
CLOYD, R.A. Ecology of fungus gnats (Bradysia spp.) in greenhouse production systems associated with disease-interactions and alternative management strategies. Insects. 2015, 6, 325–332. https://doi.org/10.3390/insects6020325
CLOYD, R.A. Management of fungus gnats (Bradysia spp.) in greenhouses and nurseries. Floricultural Ornamental Biotechnology. 2008, 2, 84–89.
CLOYD, R.A. and DICKINSON, A. Effect of Bacillus thuringiensis subsp. israelensis and neonicotinoid insecticides on the fungus gnat Bradysia sp nr. coprophila (Lintner) (Diptera: Sciaridae). Pest Management Science. 2006, 62, 171–177. https://doi.org/10.1002/ps.1143
ClOYD, R. A. and ZABORSKI, E. R. Fungus gnats, Bradysia spp. (Diptera: Sciaridae), and other arthropods in commercial bagged soilless growing media and rooted plant plugs. Horticultural Entomology. 2004, 97, 503-510. http://doi.org/10.1603/0022-0493-97.2.503
COSSENTINE, J., ROBERTSON, M. and XU, D. Biological Activity of Bacillus thuringiensis in Drosophila suzukii (Diptera: Drosophilidae). Journal of Economic Entomology. 2016, 109, 1071-1078. http://doi.org/10.1093/jee/tow062
CRICKMORE, N., et al. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiology and Molecular Biology Review. 1998, 1998, 807–813. http://doi.org/10.1128/mmbr.62.3.807-813.1998
DUARTE, A. da F., et al. Evaluation of Cosmolaelaps brevistilis and Stratiolaelaps scimitus (Mesostigmata: Laelapidae) as natural enemy of Bradysia aff. ocellaris (Diptera: Sciaridae). Systematic and Applied Acarology, 2021.
DUARTE, A. da F., et al. Compatibility of pesticides used in strawberry crops with predatory mites Stratiolaelaps scimitus (Womersley) and Cosmolaelaps brevistilis (Karg). Ecotoxicology. 2020, 29, 148–155. https://doi.org/10.1007/s10646-020-02164-w
ERLER, S., et al. Diversity of honey stores and their impact on pathogenic bacteria of the honeybee, Apis mellifera. Ecology and Evolution. 2009, 4, 3960–3967. http://doi.org/10.1002/ece3.1252
ESTRUCH, J. J., et al. Vip3A, a novel Bacillus thuringiensis vegetative insecticidal protein with a wide spectrum of activities against lepidopteran insects. Proceedings of the National Academy of Science of the United States of America. 1996, 93, 5389-5394. http://doi.org/10.1073/pnas.93.11.5389
FEDERICI, B. A., PARK, H. W., and SAKAN, Y. Insecticidal Protein Crystals of Bacillus thuringiensis. In: J. M. SHIVELY (ed.) Inclusions in Prokariotes, 2006, p. 195–293. Springer-Verlag, Berlin Heidelberg.
FRANKENHUYZEN, K. V. Insecticidal activity of Bacillus thuringiensis crystal proteins. Journal Invertebrate Pathology. 2009, 101, 1–16. https://doi.org/10.1016/j.jip.2009.02.009
FRANKENHUYZEN, K. V. Cross-order and cross-phylum activity of Bacillus thuringiensis pesticidal proteins. Journal Invertebrate Pathology. 2013, 114, 76–85. https://doi.org/10.1016/j.jip.2013.05.010
FREIRE, R.A.P., et al. Biological control of Bradysia matogrossensis (Diptera: Sciaridae) in mushroom cultivation with predatory mites. Experimental and Applied Acarology. 2007, 42, 87–93. https://doi.org/10.1007/s10493-007-9075-0
GEISLER, F.C.S., et al. Toxicity of bacterial isolates on adults of Zaprionus indianus (Diptera: Drosophilidae) and parasitoids Trichopria anastrephae (Hymenoptera: Diapriidae) and Pachycrepoideus vindemmiae (Hymenoptera: Pteromalidae). Journal of Economic Entomology. 2019, 112, 2817–2823. https://doi.org/10.1093/jee/toz218
GOUFFON, C., et al. Binding sites for Bacillus thuringiensis Cry2Ae toxin on Heliothine brush border membrane vesicles are not shared with Cry1A, Cry1F, or Vip3A toxin. Applied Environmental Microbiology. 2011, 77, 3182-3188. http://doi.org/10.1128/AEM.02791-10
ILIAS, F., et al. Insecticidal activity of Bacillus thuringiensis on larvae and adults of Bactrocera oleae Gmelin (Diptera:Tephritidae). Journal Environmental Protection. 2013, 4, 480–485. https://doi.org/10.4236/jep.2013.45056
MARTINS, L.N., et al. Biological Activity of Bacillus thuringiensis (Bacillales: Bacillaceae) in Anastrepha fraterculus (Diptera: Tephritidae). Journal Economic Entomology. 2018, 111, 1486–1489. https://doi.org/10.1093/jee/tox364
MOHRIG, W., et al. Revision of the black fungus gnats (Diptera: Sciaridae) of North America. Studia Dipterologica. 2012, 19, 141–286.
MOLINA, C.A., et al. Selection of a Bacillus pumilus strain highly active against Ceratitis capitata (Wiedemann) larvae. Applied Environmental Microbiology. 2010, 76, 1320–1327. https://doi.org/10.1128/AEM.01624-09
MOREIRA, G.F. and MORAES, G.J. de. The Potential of free-living Laelapid mites (Mesostigmata: Laelapidae) as biological control agents, In: CARRILLO et al. Prospects for biological control of plant feeding mites and other harmful organisms, 2015, pp. 77–102.
NAVROZIDIS, E.I., et al. Biological control of Bactocera oleae (Diptera: Tephritidae) using a Greek Bacillus thuringiensis isolate. Journal Economic Entomology. 2000, 93, 1657–1661. https://doi.org/10.1603/0022-0493-93.6.1657
OSBORNE, L.S., BOUCIAS, D.G. and LINDQUIST, R.K. Activity of Bacillus thuringiensis var. israelensis on Bradysia coprophila (Diptera: Sciaridae). Journal Economic Entomology. 1985, 78, 922–925. https://doi.org/10.1093/jee/78.4.922
R DEVELOPMENT CORE TEAM. R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing, 2018.
SAN-BLAS, E., et al. Biological control of the fungus gnats Bradysia difformis (Diptera, Mycetophilidae) in mushrooms with Heterorhabditis amazonensis in tropical conditions. Science Horticultural. 2017, 216, 120–125. https://doi.org/10.1016/j.scienta.2017.01.003
SAUKA, D. H., et al. PCR-based prediction of type I b-exotoxin production in Bacillus thuringiensis strains. Journal of Invertebrate Pathology. 2014, 122, 28–31. http://doi.org/10.1016/j.jip.2014.08.001
SCHNEPF, E., et al. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology Molecular Biology Reviews. 1998, 62, 775–806. https://doi.org/10.1128/mmbr.62.3.775-806.1998
SENA, J. A. D., RODRÍGUEZ, H. C. S. and FERRÉ, J., 2009. Interaction of Bacillus thuringiensis Cry1 and Vip3A proteins with Spodoptera frugiperda midgut binding sites. Applied Environmental Microbiology. 2009, 75, 2236-2237. http://doi.org/10.1128/AEM.02342-08
SHAMSHAD, A. The development of integrated pest management for the control of mushroom sciarid flies, Lycoriella ingenua (Dufour) and Bradysia ocellaris (Comstock), in cultivated mushrooms. Pest Management Science. 2010, 66, 1063–1074. https://doi.org/10.1002/ps.1987
SHAMSHAD, A., CLIFT, A.D. and MANSFIELD, S. Effect of compost and casing treatments of insecticides against the sciarid Bradysia ocellaris (Diptera: Sciaridae) and on the total yield of cultivated mushrooms, Agaricus bisporus. Pest Management Science. 2009, 65, 375–380. https://doi.org/10.1002/ps.1700
SHISHIR, M.A., et al. Novel toxicity of Bacillus thuringiensis strains against the melon fruit fly, Bactrocera cucurbitae (Diptera: Tephritidae). Biocontrol Science. 2015, 20, 115–123. https://doi.org/10.4265/bio.20.115
SOBERÓN, M., MONNERAT, R. and BRAVO, A. Mode of action of cry toxins from Bacillus thuringiensis and resistance mechanisms. Microbiology and Toxicology. 2013, 2013, 15-27
TAYLOR, M.D., WILLEY, R.D. and NOBLET, R. A 24-h potato-based toxicity test for evaluating Bacillus thuringiensis var. israelensis (H-14) against darkwinged fungus gnat Bradysia impatiens Johannsen (Diptera: Sciaridae) larvae. International Journal Pest Management. 2007, 53, 77–81. https://doi.org/10.1080/09670870601090000
TOLEDO, J., et al. Toxicity of Bacillus thuringiensis β-exotoxin to three species of fruit flies (Diptera: Tephritidae). Journal of Economic Entomology. 1999, 92, 1052–1056. https://doi.org/10.1093/jee/92.5.1052
VIDAL-QUIST, J.C., CASTAÑERA, P. and GONZÁLEZ-CABRERA, J. Diversity of Bacillus thuringiensis strains isolated from citrus orchards in Spain and evaluation of their insecticidal activity against Ceratitis capitata. Journal of Microbiology and Biotechnology. 2010, 19, 749-759. http://doi.org/10.4014/jmb.0810.595
YE, L., et al. Review of three black fungus gnat species (Diptera: Sciaridae) from greenhouses in China: Three greenhouse sciarids from China. Journal of Asia-Pacific Entomology. 2017, 20, 179–184. https://doi.org/10.1016/j.aspen.2016.12.012
WANG, F., et al. Identifcation of Cyt2Ba from a new strain of Bacillus thuringiensis and its toxicity in Bradysia diformis. Current Microbiology. 2020, 77, 2859-2866. https://doi.org/10.1007/s00284-020-02018-y
WANG, Z., et al. Comammox Nitrospira clade B contributes to nitrification in soil. Soil Biology and Biochemistry. 2019, 135, 392-395. http://doi.org/10.1016/j.soilbio.2019.06.004
ZAHIRI, N. S., TIANYUN, S. U. and MULLA, M. S. Strategies for the management of resistance in mosquitoes to the microbial control agent Bacillus sphaericus. Journal of Medical and Entomology. 2002, 39, 513-520. http://doi.org/10.1603/0022-2585-39.3.513
ZHANG, P., et al. Life table study of the effects of sublethal concentrations of thiamethoxam on Bradysia odoriphaga Yang and Zhang. Pesticide Biochemistry and Physiology. 2014, 111, 31–37. https://doi.org/10.1016/j.pestbp.2014.04.003
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Copyright (c) 2023 Adriane da Fonseca Duarte, Juliano Lessa Pinto Duarte, Liliane Nachtigall Martins, Lucas Raphael da Silva, Nycole de Souza Cunha, Fábio Pereira Leivas Leite, Uemerson Silva da Cunha, Daniel Bernardi
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