Organic onion growth, yield and storage improved by foliar sprays of microalgae and fulvic acid as a natural biofertilizer

Authors

DOI:

https://doi.org/10.14393/BJ-v38n0a2022-58854

Keywords:

Allium cepa L., Humic substances, Scenedesmus subspicatus, Organic farming, Scenedesmus subspicatus.

Abstract

The use of microalgae as natural biofertilizer in horticulture has recently been reported, while the use of humic substances is widespread. However, the combined use of microalgae with humic substances applied to plant leaves is still unexploited. Thus, the objective of this work was to combine fulvic acid (FA) with the Scenedesmus subspicatus microalga biomass (SC) as a natural biofertilizer applied via leaf in two onion cultivars in organic system. Four experiments were conducted: i) bioassays to verify the bioactive effect of FA, SC and combinations using the Vigna radiata model plant; ii) greenhouse pot experiment with foliar applications of FA, SC and combination in two onion cultivars; iii) field experiment in organic system with foliar applications of FA, SC and combination in two onion cultivars; and iv) onion bulb storage experiment. The bioactive effect of SC, FA and their combinations was identified by promoting changes in root growth of V. radiata. In pots, treatments containing FA, SC and combination promoted increase in fresh and dry leaf mass. The foliar application of FA, SC and combination promoted an increase in field bulb productivity, reduced mass loss in stored bulbs and increased carbohydrate, amino acid and protein contents in onion bulbs.

Downloads

Download data is not yet available.

References

AL-FRAIHAT, A.H., et al. Response of onion (Allium cepa) crop to foliar application of humic acid under rain-fed conditions. International Journal of Agriculture & Biology. 2018, 20(5), 1235-1241.

BETTONI, M.M., et al. Nutritional quality and yield of onion as affected by different application methods and doses of humic substances. Journal of Food Composition and Analysis. 2016, 51, 37-44. https://doi.org/10.1016/j.jfca.2016.06.008

BARONE, V., et al. Root morphological and molecular responses induced by microalgae extracts in sugar beet (Beta vulgaris L.). Journal of applied phycology. 2018, 30(2), 1061-1071. https://doi.org/10.1007/s10811-017-1283-3

BRADFORD, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry. 1976, 72, 248-254.

CANELLAS, L.P. and OLIVARES, F.L. Physiological responses to humic substances as plant growth promoter. Chemical and Biological Technologies in Agriculture. 2014, 1(1), 3.

CANELLAS, L.P., et al. Humic and fulvic acids as biostimulants in horticulture. Scientia Horticulturae. 2015, 196, 15-27. https://doi.org/10.1016/j.scienta.2015.09.013

CONSELVAN, G.B., et al. Biostimulant activity of humic substances extracted from leonardites. Plant and soil. 2017, 420(1-2), 119-134. https://doi.org/10.1007/s11104-017-3373-z

DINESHKUMAR, R.., SUBRAMANIAN, J. and SAMPATHKUMAR, P. Prospective of Chlorella vulgaris to Augment Growth and Yield Parameters Along with Superior Seed Qualities in Black Gram, Vigna mungo (L.). Waste and Biomass Valorization. 2018a, 1, 9. https://doi.org/10.1007/s12649-018-0465-9

DINESHKUMAR, R., et al. Exploring the Microalgae Biofertilizer Effect on Onion Cultivation by Field Experiment. Waste and Biomass Valorization. 2018b, 11, 77–87. https://doi.org/10.1007/s12649-018-0466-8

DU JARDIN, P. Plant biostimulants: definition, concept, main categories and regulation. Scientia Horticulturae. 2015, 196, 3-14. https://doi.org/10.1016/j.scienta.2015.09.021

DU, C.X., et al. Applying spermidine for differential responses of antioxidant enzymes in cucumber subjected to short-term salinity. Journal of the American Society for Horticultural Science. 2010, 135(1), 18-24. https://doi.org/10.21273/JASHS.135.1.18

EL-SAYED, S.A.A. Effect of potassium fertilization levels and algae extract on growth, bulb yield and quality of onion (Allium cepa L.). Middle East Journal. 2018, 7(2), 625-638.

ERTANI, A., et al. Effect of commercial lignosulfonate-humate on Zea mays L. Metabolism Journal of agricultural and food chemistry. 2011, 59, 11940–11948. https://doi.org/10.1021/jf202473e

FAN, H.M., et al. Effects of humic acid derived from sediments on growth, photosynthesis and chloroplast ultrastructure in chrysanthemum. Scientia Horticulturae. 2014, 177, 118-123. https://doi.org/10.1016/j.scienta.2014.05.010

GARCIA-GONZALEZ, J. and SOMMERFELD, M. Biofertilizer and biostimulant properties of the microalga Acutodesmus dimorphus. Journal of applied phycology. 2016, 28, 1051-1061. https://doi.org/10.1007/s10811-015-0625-2

GEMIN, L.G., et al. Microalgae associated to humic acid as a novel biostimulant improving onion growth and yield. Scientia Horticulturae. 2019, 256, 108560. https://doi.org/10.1016/j.scienta.2019.108560

ISHAQ, A.G., MATIAS-PERALTA, H.M. and BASRI, H. Bioactive Compounds from Green Microalga-Scenedesmus and its Potential Applications: A Brief Review. Pertanika Journal Tropical Agriculture Science. 2016, 39(1), 16.

KAHSAY, Y., FETIEN, A. and DERBEW, B. Intra-row spacing effect on shelf life of onion varieties (Allium cepa L.) at Aksum, Northern Ethiopia. Journal plant breeding and crop Science. 2013, 5(6), 127-136.

MADAIL, J.C.M, BELARMINO, L.C., BINI, D.A. Evolução da produção e mercado de produtos orgânicos no Brasil e no Mundo. Revista Científica Ajes. 2011, 2, 1-9.

MAGNÉ, C. and LARHER, F. High sugar content interferes with colorimetric determination of amino acids and free proline. Analytical biochemistry. 1992, 200, 4. https://doi.org/10.1016/0003-2697(92)90285-F

MALDONADE, I.R., DE CARVALHO, P.G.B. and FERREIRA, N.A. Protocolo para determinação de açúcares totais em hortaliças pelo método de DNS. Embrapa Hortaliças-Comunicado Técnico (INFOTECA-E). 2013. Available from: https://www.infoteca.cnptia.embrapa.br/bitstream/doc/956032/1/cot85.pdf

MANSOUR, F.Y., RAMADAN, M.M.A. and EL-METWALY, H.M.B. Effect of nitrogen fertilization and some stimulants on dry weight, yield, bulb quality and storability of garlic. Plant Production Science. 2019, 621, 634.

MÓGOR, Á.F., et al. Biostimulant properties of cyanobacterial hydrolysate related to polyamines. Journal of applied phycology. 2018a, 30(1), 453-460. https://doi.org/10.1007/s10811-017-1242-z

MÓGOR, Á.F., et al. Bioactivity of Cyanobacterial Biomass Related to Amino Acids Induces Growth and Metabolic Changes on Seedlings and Yield Gains of Organic Red Beet. American Journal Plant Science. 2018b, 9, 966-978. https://doi.org/10.4236/ajps.2018.95074

MORA, V., et al. Action of humic acid on promotion of cucumber shoot growth involves nitrate-related changes associated with the root-to-shoot distribution of cytokinins, polyamines and mineral nutrients. Journal of Plant Physiology. 2010, 167(8), 633-642. https://doi.org/10.1016/j.jplph.2009.11.018

OKU, S., et al. Sugar accumulation and activities of enzymes involved in fructan dynamics from seedling to bulb formation in onion (Allium cepa L.). Scientia horticulturae. 2019, 247, 147-155. https://doi.org/10.1016/j.scienta.2018.12.013

PLAZA, B.M., et al. Effect of microalgae hydrolysate foliar application (Arthrospira platensis and Scenedesmus sp.) on Petunia x hybrida growth. Journal of applied phycology. 2018, 30(4), 2359-2365. https://doi.org/10.1007/s10811-018-1427-0

RONGA, D., et al. Microalgal Biostimulants and Biofertilisers in Crop Productions. Agronomy. 2019, 9(4), 192. https://doi.org/10.3390/agronomy9040192

SHEHATA, S.A., ABDELGAWAD, K.F. and EL-MOGY, M.M. Quality and shelf-life of onion bulbs influenced by biostimulants. International Journal of Vegetable Science. 2017, 23(4), 362-371. https://doi.org/10.1080/19315260.2017.1298170

STIRK, W.A., et al. Auxin and cytokinin relationships in 24 microalgal strains. Journal of phycology. 2013, 49, 459-467. https://doi.org/10.1111/jpy.12061

SUGANYA, T., et al. Macroalgae and microalgae as a potential source for commercial applications along with biofuels production: A biorefinery approach. Renewable and Sustainable Energy Reviews. 2016, 55, 909-941. https://doi.org/10.1016/j.rser.2015.11.026

TIBBETTS, S.M., MILLEY, J.E. and LALL, S.P. Chemical composition and nutritional properties of freshwater and marine microalgal biomass cultured in photobioreactors. Journal of Applied Phycology. 2015, 27(3), 1109-1119. https://doi.org/10.1007/s10811-014-0428-x

VIDIGAL, S.M., MOREIRA, M.A. and PEREIRA, P.R.G. Crescimento e absorção de nutrientes pela planta cebola cultivada no verão por semeadura direta e por transplantio de mudas. Bioscience Journal. 2010, 26(1), 59-70.

WINTERS, A.L., et al. Evaluation of a rapid method for estimating free amino acids in silages. Animal feed science and technology. 2002, 99, 177-187. https://doi.org/10.1016/S0377-8401(02)00112-8

ZHANG, C., et al. Transcriptome analysis of sucrose metabolism during bulb swelling and development in onion (Allium cepa L.). Frontiers in Plant Science. 2016, 7, 1425. https://doi.org/10.3389/fpls.2016.01425

Downloads

Published

2022-08-12

How to Cite

GEMIN, L.G., MÓGOR, Átila F., DE OLIVEIRA AMATUSSI , J., BOCHETTI DE LARA, G. and MÓGOR, G., 2022. Organic onion growth, yield and storage improved by foliar sprays of microalgae and fulvic acid as a natural biofertilizer. Bioscience Journal [online], vol. 38, pp. e38045. [Accessed19 July 2024]. DOI 10.14393/BJ-v38n0a2022-58854. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/58854.

Issue

Section

Agricultural Sciences