Optimization of soybean yield in ultisols through adaptive varieties screening and plant growth promoting Rhizobacter


  • Elli Afrida Universitas Alwashliyah
  • Muhammad Syahril Universitas Samudra
  • Koko Tampubolon Universitas Tjut Nyak Dhien




Acid Soils, Nitrogen-Fixing Bacteria, Phosphate Solubilizing Bacteria, Screening, Soybean Varieties.


This research was aimed at obtaining varieties of soybean adaptive to acid soils and to obtain Plant Growth-Promoting Rhizobacteria (PGPR) isolates that can improve the agronomic characteristics of soybean and increase the ultisols fertility. This research was conducted in two-stages research on Sampali Village, Percut Sei Tuan sub-District, Deli Serdang District, North Sumatra Province, Indonesia from August 2019 until March 2020. The first stage (adaptive varieties screening) using the non-factorial Randomized Block Design (RBD) with the varieties of Argomulyo, Wilis, Kaba, Dena-1, Devon-1, Dega-1, Demas-1, Burangrang, Detam-1, and Kipas Merah. The second stage (application of PGPR isolates singly and in combinations) using the factorial RBD, the first factor of applicative single and the combination of PGPR isolates, the second factor of adaptive varieties including Detam-1 and Wilis. Data were analyzed with ANOVA and followed by DMRT at P<0.05. The results showed that the Detam-1 and Wilis varieties had significantly higher yield per plant of 14.73 g and 14.54 g, respectively, than other varieties. The applications of a single and combination of PGPR isolates significantly increased the number of branches, stem diameter, plant height, yield per plant, soil pH, organic-C, available-P, and total-N and decreased the soil C/N. The Detam-1 variety had the higher in yield per plant compared to Wilis variety. The isolates combination of Rhizobium leguminosarum+Rhizobium sp2+Bacillus sp+Burkholderia sp for Detam-1 and Wilis varieties can be recommended to support the growth and yield of soybean on ultisols.


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ADIE, M.M. and KRISNAWATI, A. Identification of soybean genotypes adaptive and productive to acid soil agro-ecosystem. Biodiversitas Journal of Biological Diversity. 2016, 17(2), 565-570. https://doi.org/10.13057/biodiv/d170217

ADIE, M.M. SUHARSONO and SUDARYONO. Prospects of black soybean varieties Detam-1 and Detam-2. Buletin Palawija. 2009, 18, 66-72.

AFZAL, A. and BANO, A. Rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat (Triticum aestivum). International Journal of Agriculture and Biology. 2008, 10(1), 85-88.

AHEMAD, M. and KIBRET, M. Mechanisms and applications of plant growth promoting rhizobacteria: current perspective. Journal of King Saud University-Science. 2014, 26(1), 1-20. https://doi.org/10.1016/j.jksus.2013.05.001

AHMED, N. and SHAHAB, S. Phosphate solubilization: their mechanism genetics and application. The Internet Journal of Microbiology. 2011, 9(1), 1-19. https://ispub.com/IJMB/9/1/12025

BACHTIAR, T., ANAS, I., SUTANDI, A. and ISHAK. Nitrogen uptake by mutiara-3 soybean variety as a result of application of rhizobium and phosphate solubilizer microbes. Jurnal Ilmiah Aplikasi Isotop dan Radiasi. 2019, 15(1), 7-16.

BHATTACHARYYA, P.N. and JHA, D.K. Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology. 2012, 28(4), 1327-1350. https://doi.org/10.1007/s11274-011-0979-9

CENTER FOR LAND AND AGRO-CLIMATE RESEARCH. Atlas of land resources of Indonesia at exploratory scale of 1:1,000,000. Jakarta: Soil Research Center in Ministry of Agriculture, 2000. Available from: http://en.litbang.pertanian.go.id/news/one/48/

DURESSA, D., SOLIMAN, K., TAYLOR, R. and SENWO, Z. Proteomic analysis of soybean roots under aluminum stress. International Journal of Plant Genomics. 2011, 2011, 1-12. https://doi.org/10.1155/2011/282531

ERMOLAYEV, V. Isolation of genes involved in soybean response to Al toxicity under low pH condition. In: Proceeding of workshop on soybean biotechnology for aluminum tolerance on acid soils and disease resistance. Germany: Federal Ministry for Education and Research, Central Research Institute for Food Crops, 2001, pp. 15-24.

EVANS, J.D. Straightforward statistics for the behavioral sciences. California: Thomson Brooks/Cole Publishing Company, 1996.

EZEH, K.N., OMOGOYE, A.M. and AKINRINDE, E.A. Aluminum influence on performance of some cowpea (Vigna unguiculata) varieties on a Nigerian Alfisol. World Journal of Agricultural Sciences. 2007, 3(4), 517-522.

GANGASURESH, P., et al. Synergistic efficiency of phosphate solubilizer associated with nitrogen fixer on the growth of soybean (Glycine max). International Journal of Biological Technology. 2010, 1(2), 124-130.

GIORDANO, W. and HIRSCH, A.M. The expression of MaEXP1, a Melilotus alba expansin gene, is upregulated during the sweetclover-Sinorhizobium meliloti interaction. Molecular Plant-Microbe Interactions. 2004, 17(6), 613-622. https://doi.org/10.1094/MPMI.2004.17.6.613

HOLT, J.G., KRIEG, N.R., SNEATH, P.H.A. and STALEY, J.T. Bergey’s manual of determinative bacteriology. 9th Edition. USA: Williams & Wilkins, 1994.

HORNER, E.R. The effect of nitrogen application timing on plant available phosphorus. Columbus: The Ohio State University, 2008. Thesis.

INDONESIA SOIL RESEARCH INSTITUTE. Technical guide 2: chemical analysis of soil, plants, water and fertilizer. Bogor, Indonesia: Soil Research Institute, Ministry of Agriculture, 2009, p. 246.

JIANG, C.Y., SHENG, X.F., QIAN, M. and WANG, Q.Y. Isolation and characterization of a heavy metal-resistant Burkholderia sp. from heavy metal-contaminated paddy field soil and its potential in promoting plant growth and heavy metal accumulation in metal-polluted soil. Chemosphere. 2008, 72(2), 157-164. https://doi.org/10.1016/j.chemosphere.2008.02.006

KAFRAWI. Screening of Plant Growth Promoting Rhizobacteri (PGPR) isolates from shallots (Allium ascalonicum) in Gorontalo. Prosiding Seminar Nasional Biologi. 2015, 1(1), 132-139.

KRISNAWATI, A. and ADIE, M.M. Relationship between morphological components with seed yield characters of soybean. Buletin Palawija. 2016, 14(2), 49-54. http://dx.doi.org/10.21082/bulpa.v14n2.2016.p49-54

MINISTRY OF AGRICULTURE. Description of superior soybean varieties 1918-2016. Jakarta: Crop Research Institute of Beans and Tubers, 2016. Available from: http://balitkabi.litbang.pertanian.go.id/publikasi/deskripsi-varietas/#

MINISTRY OF AGRICULTURE. Statistics of agricultural land 2014-2018. Jakarta: Center for Agriculture Data and Information System, 2019. Available from: http://epublikasi.setjen.pertanian.go.id/arsip-perstatistikan/167-statistik/statistik-lahan/652-statistik-data-lahan-pertanian-tahun-2014-2018

MISHRA, P.K., et al. Enhanced soybean (Glycine max L.) plant growth and nodulation by Bradyrhizobium japonicum-SB1 in presence of Bacillus thuringiensis-KR1. Acta Agriculturae Scandinavica Section B–Soil and Plant Science. 2009, 59(2), 189-196. https://doi.org/10.1080/09064710802040558

MITTAL, V., et al. Stimulatory effect of phosphate-solubilizing fungal strains (Aspergillus awamori and Penicillium citrinum) on the yield of chickpea (Cicer arietinum L. cv. GPF2). Soil Biology and Biochemistry. 2008, 40(3), 718-727. https://doi.org/10.1016/j.soilbio.2007.10.008

MOSSOR-PIETRASZEWSKA, T. Effect of aluminium on plant growth and metabolism. Acta Biochimica Polonica. 2001, 48(3), 673-686. https://doi.org/10.18388/abp.2001_3902

MULYANI, A., HIKMATULLAH and SUBAGYO, H. Characteristics and potential of acid dry land in Indonesia. In: Prosiding simposium nasional pendayagunaan tanah masam, Bandar Lampung, Indonesia, 2003.

NAIBAHO, D., HANAFIAH, D.S. and TAMPUBOLON, K. Stress susceptibility index and heritability of tomato varieties to aluminum-treatment with nutrient culture media. International Journal of Scientific and Technology Research. 2019, 8(9), 17-23.

PIKOVSKAYA, R.I. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya. 1948, 17, 362-370.

PRASETYO, B.H. and SURIADIKARTA, D.A. Characteristics, potential, and management of ultisols for agrilcultural upland development in Indonesia. Jurnal Litbang Pertanian. 2006, 25(2), 39-46.

RAO, N.S. Soil microorganisms and plant growth. Jakarta: Universitas Indonesia Press, 1994.

RODRÍGUEZ, H. and FRAGA, R. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances. 1999, 17(4-5), 319-339. https://doi.org/10.1016/S0734-9750(99)00014-2

SANTANA, E.B., MARQUES, E.L.S. and DIAS, J.C.T. Effects of phosphate-solubilizing bacteria, native microorganisms, and rock dust on Jatropha curcas L. growth. Genetics and Molecular Research. 2016, 15(4), 1-18. https://doi.org/10.4238/gmr.15048729

SATYAPRAKASH, M., et al. Phosphorous and phosphate solubilising bacteria and their role in plant nutrition. International Journal of Current Microbiology and Applied Sciences. 2017, 6(4), 2133-2144. https://doi.org/10.20546/ijcmas.2017.604.251

SCHMIDT, J., MESSMER, M. and WILBOIS, K.P. Beneficial microorganisms for soybean (Glycine max (L.) Merr), with a focus on low root-zone temperatures. Plant and Soil. 2015, 397(1-2), 411-445. https://doi.org/10.1007/s11104-015-2546-x

SHAHAB, S., AHMED, N. and KHAN, N.S. Indole acetic acid production and enhanced plant growth promotion by indigenous PSBs. African Journal of Agricultural Research. 2009, 4(11), 1312-1316.

STATISTICS OF SUMATERA UTARA. North Sumatra Province in figure 2019. Medan, Indonesia: Badan Pusat Statistik Provinsi Sumatera Utara, 2019. Available from: https://sumut.bps.go.id/statictable/2020/06/10/1969/luas-panen-rata-rata-produksi-dan-produksi-kacang-kedelai-2008-2019.html

SUBAGYO, H., SUHARTA, N. and SISWANTO, A.B. Agricultural lands in Indonesia. Jakarta, Indonesia: Center for Land and Agro-climate Research, Indonesian Agency for Agricultural Research and Development, Ministry of Agriculture, 2004, p. 21-65.

TAUFIQ, A., KUNTYASTUTI, H., PRAHORO, C. and WARDANI, T. Lime and manure application on soybean on acid dry land. Jurnal Penelitian Pertanian Tanaman Pangan. 2007, 26(1), 78-85.

THAKURIA, D., et al. Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of Assam. Current Science. 2004, 86(07), 978-985.

TONELLI, M.L., MAGALLANES-NOGUERA, C. and FABRA, A. Symbiotic performance and induction of systemic resistance against Cercospora sojina in soybean plants co-inoculated with Bacillus sp. CHEP5 and Bradyrhizobium japonicum E109. Archives of Microbiology. 2017, 199(9), 1283-1291. https://doi.org/10.1007/s00203-017-1401-2

UGURU, M.I., OYIGA, B.C. and JANDONG, E.A. Responses of some soybean genotypes to different soil pH regimes in two planting seasons. The African Journal of Plant Science and Biotechnology. 2012, 6(1), 26-37.

VACHERON, J., et al. Plant growth-promoting rhizobacteria and root system functioning. Frontiers in Plant Science. 2013, 4, 356. http://dx.doi.org/10.3389/fpls.2013.00356

VIKRAM, A. and HAMZEHZARGHANI, H. Effect of phosphate solubilizing bacteria on nodulation and growth parameters of greengram (Vigna radiata L. Wilczek). Research Journal of Microbiology. 2008, 3(2), 62-72. http://dx.doi.org/10.3923/jm.2008.62.72

WALPOLA, B.C. and YOON, M.H. Prospectus of phosphate solubilizing microorganisms and phosphorus availability in agricultural soils: A review. African Journal of Microbiology Research. 2012, 6(37), 6600-6605. https://doi.org/10.5897/AJMR12.889

WANG, X., et al. Phosphorus acquisition characteristics of cotton (Gossypium hirsutum L.), wheat (Triticum aestivum L.) and white lupin (Lupinus albus L.) under P deficient conditions. Plant and Soil. 2008, 312(1-2), 117-128. https://doi.org/10.1007/s11104-008-9589-1

WANI, P.A. and KHAN, M.S. Bacillus species enhance growth parameters of chickpea (Cicer arietinum L.) in chromium stressed soils. Food and Chemical Toxicology. 2010, 48(11), 3262-3267. https://doi.org/10.1016/j.fct.2010.08.035

WANI, P., KHAN, M. and ZAIDI, A. Co-inoculation of nitrogen-fixing and phosphate-solubilizing bacteria to promote growth, yield and nutrient uptake in chickpea. Acta Agronomica Hungarica. 2007, 55(3), 315-323. https://doi.org/10.1556/AAgr.55.2007.3.7

WEISANY, W., RAEI, Y. and ALLAHVERDIPOOR, K.H. Role of some of mineral nutrients in biological nitrogen fixation. Bulletin of Environment, Pharmacology and Life Sciences. 2013, 2(4), 77-84.

WOLFF, A.B., SINGLETON, P.W., SIDIRELLI, M. and BOHLOOL, B.B. Influence of acid soil on nodulation and interstrain competitiveness in relation to tannin concentrations in seeds and roots of Phaseolus vulgaris. Soil Biology and Biochemistry. 1993, 25(6), 715-721. https://doi.org/10.1016/0038-0717(93)90112-O

YADAV, M.R., et al. Strategies for improving nitrogen use efficiency: A review. Agricultural Reviews. 2017, 38(1), 29-40. http://dx.doi.org/10.18805/ag.v0iOF.7306

YOUSEFI, A.A., et al. Phosphate solubilizing bacteria and arbuscular mycorrhizal fungi impacts on inorganic phosphorus fractions and wheat growth. World Applied Sciences Journal. 2011, 15(9), 1310-1318.

ZAHIR, Z.A., SHAH, M.K., NAVEED, M. and AKHTER, M.J. Substrate-dependent auxin production by Rhizobium phaseoli improves the growth and yield of Vigna radiata L. under salt stress conditions. Journal of Microbiology and Biotechnology. 2010, 20(9), 1288-1294. https://doi.org/10.4014/jmb.1002.02010




How to Cite

AFRIDA, E., SYAHRIL, M.. and KOKO TAMPUBOLON, 2022. Optimization of soybean yield in ultisols through adaptive varieties screening and plant growth promoting Rhizobacter. Bioscience Journal [online], vol. 38, pp. e38013. [Accessed10 August 2022]. DOI 10.14393/BJ-v38n0a2022-57214. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/57214.



Agricultural Sciences