Effect of reciprocating instrumentation on chlorhexidine substantivity on human dentin:  chemical analysis followed by confocal laser microscopy

Matheus Albino Souza; Fernanda Duda Bonatto; Afonso Cristiano Fleck da Silva; Ezequiel Santin Gabrielli; Felipe Trentin Motter; Larissa  Piuco; Karen Barea de Paula Duarte; Charise Dallazem  Bertol; Luciana Grazziotin Rossato-Grando; Carlo Theodoro Raimundy  Lago; Doglas Cecchin; Juliane  Bervian; João Paulo De Carli; Francisco Montagner

doi:10.14393/BJ-v37n0a2021-54518

Authors

Matheus Albino Souza Universidade de Passo Fundo https://orcid.org/0000-0002-9412-3807
Fernanda Duda Bonatto Consultório particular https://orcid.org/0000-0002-4873-4287
Afonso Cristiano Fleck da Silva Consultório particular https://orcid.org/0000-0001-8933-9817
Ezequiel Santin Gabrielli Universidade de Passo Fundo https://orcid.org/0000-0002-9825-4222
Felipe Trentin Motter Consultório particular https://orcid.org/0000-0003-2285-8027
Larissa Piuco Consultório particular https://orcid.org/0000-0003-4680-9991
Karen Barea de Paula Duarte Universidade Federal do Rio Grande do Sul https://orcid.org/0000-0002-6779-2765
Charise Dallazem Bertol Universidade de Passo Fundo https://orcid.org/0000-0001-7596-315X
Luciana Grazziotin Rossato-Grando Universidade de Passo Fundo https://orcid.org/0000-0003-4574-855X
Carlo Theodoro Raimundy Lago Universidade de Passo Fundo https://orcid.org/0000-0002-9018-5373
Doglas Cecchin Universidade de Passo Fundo https://orcid.org/0000-0001-8851-5233
Juliane Bervian Universidade de Passo Fundo https://orcid.org/0000-0001-5718-3539
João Paulo De Carli Universidade de Passo Fundo https://orcid.org/0000-0002-4705-6226
Francisco Montagner Universidade Federal do Rio Grande do Sul https://orcid.org/0000-0002-7850-0107

DOI:

https://doi.org/10.14393/BJ-v37n0a2021-54518

Keywords:

Chromatography, Enterococcus faecalis, Ex vivo, Human Tooth, Root Canal Preparation.

Abstract

The present research analyzed the reciprocating instrumentation associated to chlorhexidine (CHX) substantivity as its correlation with E. faecalis viability in ex vivo root canals. Eighty extracted single-rooted human teeth were used, being 40 to high-performance liquid chromatography (HPLC) and 40 to confocal laser scanning microscopy (CLSM). In both, teeth were decoronated and the cervical third was prepared. In the CLSM analysis, the root canals were inoculated with E. faecalis for 14 days. Samples were divided into 4 groups (n=10) according to instrumentation technique: no instrumentation and irrigation with distilled water (control); manual instrumentation (K-File); rotary instrumentation (ProTaper Next); and reciprocating instrumentation (Reciproc R25). Two percent chlorhexidine was applied as irrigating substance in experimental groups. Longitudinal grooves resulted in 2 halves root and 20 proof bodies in each group. Samples were divided by chance in two groups (n=10) and the outcomes were evaluated after two days and one week. The retained chlorhexidine and live cells after instrumentation techniques in each evaluation time was measured by HPLC and CLSM, respectively. Specific analysis was applied for experimental tests (p≤0.05). Both rotary as well as reciprocating techniques significantly reduced the amount of chlorhexidine on dentin in all observation periods (p<0.05). After evaluation times, all experimental groups presented lower live cells compared to control, but without statistically difference. Intragroup comparisons in times of evaluation showed no differences in instrumentation techniques, in chlorhexidine retention and number of live cells (p>0.05). Reciprocating instrumentation does not interfere on chlorhexidine substantivity.

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