Effect of heavy metals on stem anatomical characteristics of Trapa natans L. from Skadar Lake (Montenegro)





Anatomy, Heavy metals, Stem, Trapa natans L.


This study aimed to analyze stem anatomical characteristics of Trapa natans from five Skadar Lake locations (L1 - Milovića bay, L2 – inflow of the Morača river, L3 - Kamenik, L4 - Grmožur, L5 - Lipovik) with different concentrations of Cu, Mn, Zn, Co and Pb, during the summer period of the year 2012. Cross sections of stem were made using cryotechnic procedure. For all analyzed quantitative anatomical parameters, the minimum values at the location L2 were recorded, with the presence of maximum content for all investigated metals in stem of Trapa natans. On the other hand, except for cobalt, at the location L4 the minimum concentration for all investigated metals were recorded. Plants collected from this location have the largest average values of the most measured anatomical parameters. The results of Discriminant Analysis showed that plants from different location could be clearly classified into three groups according to their stem quantitative anatomical characteristics which corresponds with heavy metals content. Our research also showed that there is no statistically significant correlation between the content of most investigated metals (Co, Cu, Pb and Zn) and the values of anatomical parameters. Statistically significant negative correlation was found between Mn content in stem and values of two anatomical characters (stem cross-sectional area and Mn content, r = - 0.88; p < 0.05; number of hypodermal cell layers and Mn content, r = - 0.90; p < 0.05).



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Al-SAADI, S.A.A.M., Al-ASAADI, W.M. and Al-WAHEEB, A.N.H. The effect of some heavy metals accumulation on physiological and anatomical characteristic of some Potamogeton L. plant. Journal of Ecology and Environmental Sciences. 2013, 4, 100-108.

BALDANTONI, D., et al. Assessment of macro and microelement accumulation capability of two aquatic plants. Environmental Pollution. 2004, 130, 149–156. https://doi.org/10.1016/j.envpol.2003.12.015

BALDISSEROTTO, C., et al. Responses of Trapa natans L. Floating laminae to high concentrations of manganese. Protoplasma. 2007, 231, 65 –82. https://doi.org/10.1007/s00709-007-0242-2

BOES, X., et al. Evaluation of conservative lithogenic elements (Ti, Zr. Al and Rb) to study anthropogenic element enrichments in lake sediments. Journal of Paleolimnology. 2011, 46, 75-87. https://doi.org/10.1007/s10933-011-9515-z

BOWLER, C., VAN CAMP, W., VAN MONTAGU, M. and INZE, D. Superoxide dismutase in plants. Critical Reviews in Plant Sciences. 1994, 13, 199–218. https://doi.org/10.1080/07352689409701914

BUCHEL, C., et al. Photo assembly of the manganese cluster and oxygen evolution frommonomeric and dimeric CP47-reaction center photosystem II complexes. Proceedings of the National Academy of Sciences USA 96. 1999, 96(25), 14288–14293. https://doi.org/10.1073/pnas.96.25.14288

BUTA, B., et al. The Influence of Heavy Metals on Growth and Development of Eichhornia crassipes Species, Cultivated in Contaminated Water. Notulae Botanicae Horti Agrobotanici. 2011, 39(2), 135-141. https://doi.org/10.15835/nbha3926095

CARDWELL, A.J., HAWKER, D.W. and GREENWAY, M. Heavy metal accumulation in aquatic macrophytes from southeast Queensland, Australia. Chemosphere. 2002, 48, 653–663.

CLEMENS, S., PALMGEREN, M.G. and KRAMER, U. A long way ahead: Understand and engineering plant metal accumulation. Trends in Plant Science. 2002, 7, 309-315.

ENT, A.V.D., et al. Hyper accumulators of metal and metalloid trace elements: facts and fiction. Plant and Soil. 2013, 362(1-2), 319-334. https://doi.org/10.1007/s11104-012-1287-3

FARZADFAR, S. and ZARINKAMAR, F. Morphological and anatomical responses of Matricaria chamomilla plants to cadmium and calcium. Advances in Environmental Biology. 2012, 6(5), 1603-1609.

GUILIZZONI, P. The role of heavy metals and toxic materials in the physiological ecology of submeresed macrophytes. Aquatic Botany. 1991, 41, 87-109. https://doi.org/10.1016/0304-3770(91)90040-C

GUPTA, S. and CHAKRABARTI, S.K. Effect of heavy metals on different anatomical structures of Bruguiera sexangula. Internacional Journal of Bio-resource and Stress Management. 2013, 4(4), 605-609.

KABATA-PENDIAS, A. Trace elements in soils and plants. 4th ed. Boca Raton, London, NewYork: CRC Press, Taylor and Francis Group, LLC, 2011.

KASIM, W.A. Changes induced by cooper and cadmium stress in the anatomy and grain yield of Sorghum bicolor (L.)Moench. International Journal of Agriculture and Biology. 2006, 8, 123-128.

KASTRATOVIĆ, V., KRIVOKAPIĆ, S., ĐUROVIĆ, D. and BLAGOJEVIĆ, N. Seasonal changes in metal accumulation and distribution in the organs of Phragmites australis (common reed) from Lake Skadar, Montenegro. Journal of the Serbian Chemical Society. 2013, 78, 1241–1258. https://doi.org/10.2298/JSC121026153K

KASTRATOVIĆ, V., et al. Bioaccumulation and translocation of heavy metals by Ceratophyllum demersum from the Skadar Lake, Montenegro. Journal of the Serbian Chemical Society. 2014, 79, 1445–1460. https://doi.org/10.2298/jsc140409074k

KASTRATOVIĆ, V., et al. Seasonal patterns of Cu in a system of sediment-water-macrophytes. Fressenius Environmental Bulletin. 2017, 26(2), 1247-1253.

KASTRATOVIĆ, V., et al. Levels and distribution of cobalt and nickel in the aquatic macrophytes found in Skadar Lake, Montenegro. Environmental Science and Pollution Research. 2018, 25(27), 26823-26830.

KUMAR, J.I.N., SONI, H. and KUMAR, R.N. Biomonitoring of selected freshwater macrophytes to assess lake trace element contamination: a case study of Nal Sarvar Bird Sanctuary, Gujarat, India. Journal of Limnology. 2006, 65, 9-16. https://doi.org/10.4081/jlimnol.2006.9

KUMAR, M., CHIKARA, S., CHAND, M.K. and BHATNAGAR, A.K. Accumulation of lead, cadmium, zinc and copper in the edible aquatic plants Roxb. and Nelumbo nucifera Gaertn. Bulletin of Environmental Contamination and Toxicology. 2002, 69, 649–654. https://doi.org/10.1007/s00128-002-0110-x

MARCHAND, L., MENCH M., JACOB, D.L. and OTTE, M.L. Metal and metalloid removal in constructed wetlands, with emphasis on the importance of plants and standardized measurements: A review. Environmental Pollution. 2010, 158, 3447–3461. http://dx.doi.org/10.1016/j.envpol.2010.08.018

PANDEY, N., PATKAK, G.C., PANDEY, D.K. and PANDEY, R. Heavy metals, Co, Ni, Cu, Zn and Cd, produce oxidative damage and evoke differential antioxidant responses in spinach. Brazilian Journal of Plant Physiology. 2009, 21(2), 103–111. https://doi.org/10.1590/S1677-04202009000200003

PETROVIĆ, D., et al. Aquatic plant Trapa natans L. as bioindicator of trace metal contamination in a freshwater lake (Skadar Lake, Montenegro). Acta Botanica Croatica. 2016, 75(2), 236-243. https://doi.org/10.1515/botcro-2016-0031

RAI, P.K. Heavy metal phytoremediation ftom aquatic ecosystem with special reference to macrophytes. Critical Review in Environmental Science and Technology. 2009, 39, 697-753. https://doi.org/10.1080/10643380801910058

RAI, U.N., SINHA, S. and CHANDRA, P. Metal biomonitoring in water resources of Eastern Ghats, Karaput (Orissa), India by aquatic plants. Environmental Monitoring and Assessment. 1996, 43, 125–137. https://doi.org/10.1007/BF00398603

RAI, U.N. and SINHA, S. Distribution of metals in aquatic edible plants Trapa natans (Roxb.) Makino and Ipomoea aquatica Forsk. Environmental Monitoring and Assessment. 2001, 70, 241–252. https://doi.org/10.1023/a:1010727325662

SANTANDREA, G., PANDOLFI, T. and BENNICI, A. A physiological characterization of Mn-tolerant tobacco plants selected by in vitro culture. Plant Science. 2000, 150, 163–170.

STEŠEVIĆ, D., et al. Application of a new sediment contact test with Myriophyllum aquaticum and of the aquatic Lemna test to assess the sediment quality of Lake Skadar. Journal of Soils and Sediments. 2007, 7, 342–349. http://doi.org/10.1065/jss2007.08.249

SWETA, BAUDDH, K., SINGH, R.P. and SINGH, R.P. The suitability of Trapa natans for phytoremediation of inorganic contaminants from the aquatic. Ecological Engineering. 2015, 9, 39–42. http://doi.org/10.1016%2Fj.ecoleng.2015.06.003

WANG, Q., CUI, Y. and DONG, Y. Phytoremediation of polluted waters Potentials and prospects of wetland plants. Acta Biotechnological. 2002, 22, 199–208. http://doi.org/10.1002/1521-3846(200205)22:1/2<199

WHITTON, B. and KELLY, M. Use of algae and other plants for monitoring rivers. Australian Journal of Ecology. 1995, 20, 45 –56. https://doi.org/10.1111/j.1442-9993.1995.tb00521.x

VEMIĆ, M., ROUSSEAU, D., DU LAING, G. and LENS, P. Distribution and fate of metals in the Montenegrin part of Lake Skadar. International Journal. 2016, 5(1), 39-48. https://doi.org/10.1016/j.fshw.2015.11.003




How to Cite

PETROVIĆ, D., KRIVOKAPIĆ , S.., ANAČKOV, G. and LUKOVIĆ, J., 2021. Effect of heavy metals on stem anatomical characteristics of Trapa natans L. from Skadar Lake (Montenegro). Bioscience Journal [online], vol. 37, pp. e37083. [Accessed14 August 2022]. DOI 10.14393/BJ-v37n0a2021-54073. Available from: https://seer.ufu.br/index.php/biosciencejournal/article/view/54073.



Biological Sciences